LPS对奶牛子宫内膜细胞免疫相关基因表达影响的研究
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- 英文论文题名：Effects of LPS on the expression of immune related genes in bovine endometrial cells
- 论文作者：燕晓晓 ; 刘红羽 ; 安冬 ; 董杨云逸 ; 王军 ; 吕文发
- 英文论文作者：YAN Xiao-xiao ; LIU Hong-yu ; AN Dong ; DONG Yang-yun-yi ; WANG Jun ; LYU Wen-fa ; College of Animal Science and Technology ; Jilin Agricultural University
- 年：2016
- 作者机构：吉林农业大学动物科技学院;
- 论文关键词：奶牛子宫内膜炎 ; 免疫相关基因 ; LPS ; ELISA ; 荧光定量PCR
- 英文论文关键词：bovine endometritis ; immune related genes ; LPS ; ELISA ; RT-PCR
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：S858.23
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：76k
- 原文格式：O
- 会议级别：全国
摘要
明确LPS对奶牛子宫内膜细胞免疫相关基因表达的影响。建立基于组织块培养法的奶牛子宫内膜细胞培养体系,利用免疫荧光法分别检测角蛋白和波形蛋白表达鉴定上皮细胞和基质细胞。LPS处理细胞24h后,ELISA法检测细胞培养液上清中IL-6和IL-8的分泌变化,荧光定量PCR法检测IL-1队PDE4B、Nur77、P38α、COX2和FLT1基因表达变化。与对照组相比,LPS处理24h可显著提高细胞几-6和IL-8的分泌量及IL-1α、PDE4B、Nur77、P38α、COX2和FLT1的mRNA表达量。IL-6、IL-8、IL-1α、PDE4B、Nur77、P38α、COX2和FLT1基因可能在LPS诱导的奶牛子宫内膜细胞炎症反应中起重要的作用。
The objective of this study was to evaluate the differential expression of immune related genes in bovine endometrial cells.Establishment of the bovine endometrial cells culture system based on tissue explants culture method,the cytokeratin and vimentin were detected by immunofluorescence to identify epithelial and stromal.The cells were stimulated by LPS for 24 h followed by the detection of IL-6 and IL-8 by ELISA in the cell supernatant and the differential expression of IL-1α,PDE4 B,Nur77,p38α,COX2 and Flt1 by RT-PCR.The treatment of LPS for 24 h can significantly up-regulated the IL-6 and IL-8 secretion and PDE4 B,Nur77,P38,COX2,IL-1α and FLT1 mRNA epression compared with the control group.The IL-6,IL-8,IL-1,PDE4 B,Nur77,p38 a,COX2 and FLT1 may play an important role in the inflammatory response of bovine endometrial cells induced by LPS.
The objective of this study was to evaluate the differential expression of immune related genes in bovine endometrial cells.Establishment of the bovine endometrial cells culture system based on tissue explants culture method,the cytokeratin and vimentin were detected by immunofluorescence to identify epithelial and stromal.The cells were stimulated by LPS for 24 h followed by the detection of IL-6 and IL-8 by ELISA in the cell supernatant and the differential expression of IL-1α,PDE4 B,Nur77,p38α,COX2 and Flt1 by RT-PCR.The treatment of LPS for 24 h can significantly up-regulated the IL-6 and IL-8 secretion and PDE4 B,Nur77,P38,COX2,IL-1α and FLT1 mRNA epression compared with the control group.The IL-6,IL-8,IL-1,PDE4 B,Nur77,p38 a,COX2 and FLT1 may play an important role in the inflammatory response of bovine endometrial cells induced by LPS.
引文
[1].Galvao KN,Santos NR,Galvao JS,Gilbert RO.Association between endometritis and endometrial cytokine expression in postpartum Holstein cows[J].Theriogenology.2011;76(2):290-9.
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[3].Cronin JG,Turner ML,Goetze L,Bryant CE,Sheldon IM.Toll-like receptor 4 and MYD88-dependent signaling mechanisms of the innate immune system are essential for the response to lipopolysaccharide by epithelial and stromal cells of the bovine endometrium[J].Biology of reproduction.2012;86(2):51.
[4].Florio P,Rossi M,Vigano P,Luisi S,Torricelli M,Torres PB,et al.Interleukin 1beta and progesterone stimulate activin a expression and secretion from cultured human endometrial stromal cells[J].Reproductive sciences(Thousand Oaks,Calif).2007;14(1):29-36.
[5].Gabler C,Fischer C,Drillich M,Einspanier R,Heuwieser W.Time-dependent mRNA expression of selected pro-inflammatory factors in the endometrium of primiparous cows postpartum[J].Reproductive biology and endocrinology:RB&E.2010;8:152.
[2].杨一新,李桂源.LPS所介导的信号转导通路研究进展[J].中南大学学报(医学版).2006(01):141-5
[3].Cronin JG,Turner ML,Goetze L,Bryant CE,Sheldon IM.Toll-like receptor 4 and MYD88-dependent signaling mechanisms of the innate immune system are essential for the response to lipopolysaccharide by epithelial and stromal cells of the bovine endometrium[J].Biology of reproduction.2012;86(2):51.
[4].Florio P,Rossi M,Vigano P,Luisi S,Torricelli M,Torres PB,et al.Interleukin 1beta and progesterone stimulate activin a expression and secretion from cultured human endometrial stromal cells[J].Reproductive sciences(Thousand Oaks,Calif).2007;14(1):29-36.
[5].Gabler C,Fischer C,Drillich M,Einspanier R,Heuwieser W.Time-dependent mRNA expression of selected pro-inflammatory factors in the endometrium of primiparous cows postpartum[J].Reproductive biology and endocrinology:RB&E.2010;8:152.