硝酸镧对长柄扁桃试管苗生根的影响
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- 英文论文题名：Effect of La(No_3)_3 on Root Growth of Amygdalus pedunculata Pall.
- 论文作者：郭丽珠 ; 陈邦 ; 周攀 ; 何玮 ; 樊鹏辉 ; 郭斌 ; 尉亚辉 ; 申烨华 ; 李聪
- 英文论文作者：Guo Lizhu ; Chen Bang ; Zhou Pan ; He Wei ; Fan Penghui ; Guo Bin ; Wei Yahui ; Shen Yehua ; Li Cong ; Key Laboratory of Resource Biology and Biotechnology in Western China ; Ministry of Education ; Shanxi Provincial Key Laboratory of Biotechnology ; College of Life Science ; Northwest University
- 年：2014
- 作者机构：西部资源生物与现代生物技术省部共建教育部重点实验室陕西省生物技术重点实验室西北大学生命科学学院;
- 论文关键词：长柄扁桃 ; 硝酸镧 ; 试管苗 ; 生根
- 英文论文关键词：Amygdalus pedunculata Pall. ; La(NO_3)_3 ; tube plantlet ; rooting
- 会议召开时间：2014-11-29
- 会议录名称：陕西省毒理学会2014年学术会议论文集
- 语种：中文
- 分类号：S662.9
- 学会代码：XBST
- 会议名称：陕西省毒理学会2014年学术会议
- 会议地点：中国陕西西安
- 主办单位：陕西省毒理学会
- 学会名称：西北大学生态毒理研究所
- 页数：10
- 文件大小：1337k
- 原文格式：O
- 会议级别：地方
摘要
通过极差分析研究不同浓度的硝酸镧和生长素组合对长柄扁桃试管苗生根的影响。结果表明:对长柄扁桃生根影响的主次因素为硝酸镧(La(NO_3)_3)>吲哚丁酸(IBA)>吲哚乙酸(IAA),最优组合为La(NO_3)_3 20 mg·L~(-1)+IBA 0.2 mg·L~(-1)+IAA 0.2 mg·L~(-1)。在此条件下培养42 d后,长柄扁桃试管苗根的诱导频率达到89%、平均根长达到9.2 cm、每株根的平均个数为9.7个。对长柄扁桃的根组织活力进行测定,结果表明添加镧的处理组根系还原力是对照(不加镧)组的1.57倍。经过移栽驯化2个月后,长柄扁桃组培苗的移栽成活率达到94%,株高是对照的1.53倍。
Using Amygdalus pedunculata Pall,as explants,the effects of La(NO_3)_3 and auxin of different concentrations on the multiplication of A.pedunculata Pall,were studied.The results by range analysis indicated that the effective order of three factors was La(NO_3)_3 > IBA > IAA.The optimal combination was La(NO_3)_3 20 mg·L~(-1) + IBA 0.2 mg·L~(-1) + IAA 0.2 mg·L~(-1).42 days under the optimal growth condition,the induction rate reached 89%,the average root length was 9.2 cm,and the number of roots per plant reached 9.7.The highest survival rate of the domesticated transplant seedlings could reach 94%,and plant height is 1.53 times of the control.
Using Amygdalus pedunculata Pall,as explants,the effects of La(NO_3)_3 and auxin of different concentrations on the multiplication of A.pedunculata Pall,were studied.The results by range analysis indicated that the effective order of three factors was La(NO_3)_3 > IBA > IAA.The optimal combination was La(NO_3)_3 20 mg·L~(-1) + IBA 0.2 mg·L~(-1) + IAA 0.2 mg·L~(-1).42 days under the optimal growth condition,the induction rate reached 89%,the average root length was 9.2 cm,and the number of roots per plant reached 9.7.The highest survival rate of the domesticated transplant seedlings could reach 94%,and plant height is 1.53 times of the control.
引文
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[3]Chu J,Xu X,Zhang Y.Production and properties of biodiesel produced from Amygdalus pedunculata Pall.[J].Bioresource technol.,2013,4(134):374.
[4]孙占育.长柄扁桃茎尖离体培养研究[J].安徽农业科学,2010,38(19):9988.Sun Z Y.Study on micropropagation of Amygdalus pedunculata Pall.Shoot-tips in vitro[J].Journal of Anhui Agricultural Sciences,2010,38(19):9988.
[5]巩东辉,李国龙,张少英,陈涛.外源稀土La~(3+)对鄂尔多斯高原碱湖钝顶节旋藻生长及生理特性的影响[J].中国稀土学报,2011,29(4):504.Gong D H,Li G L,Zhang S Y,Chen T.Effects of external rare earth La~(3+)on growth and physiological property of Arthrospira in alkaline lake of Erdos plateau[J].Journal of the Chinese Society of Rare Earths,2011,29(4):504.
[6]张子学,胡红德,胡能兵,张从宇,崔广荣.稀土对NaCl胁迫下矮牵牛幼苗生长抑制的缓解效应[J].中国稀土学报,2010,28(6):760.Zhang Z X,Hu H D,Hu N B,Zhang C Y,Cui G R.Mitigation effect of rare-earth on seeding growth and rooting of Petunia under NaCl Stress[J].Journal of the Chinese Society of Rare Earths,2010,28(6):760.
[7]李树蕾,陈曦,聂毓秀,黄可欣.长期口服硝酸稀土对大鼠腺垂体生长激素细胞的影响[J].中国稀土学报,2003,21(6):691.Li S L,Chen X,Nie Y X,Huang K X.Effect of rare earth nitrate administrated long-term orally on rat somatotroph in adenohypophysis[J].Journal of the Chinese Society of Rare Earths,2003,21(6):691.
[8]陈远孟,白厚义,李杨瑞.镧在主要农作物上的应用及其生理生化作用[J].广西农业科学,2003,(6):15.Chen Y M,Bai H Y,Li Y R.Application and physiological and biochemical effects of lanthanum on major crops[J].Guangxi Agricultural Sciences,2003,(6):15.
[9]Hu Z,Richter H,Sparovek G,Schnug E.Physiological and biochemical effects of rare earth elements on plants and their agricultural significance:a review[J].J.Plant Nutr.,2004,(27):183.
[10]谢祖彬,朱建国,褚海燕,张雅丽,高人,曾青,曹志洪.分根法研究镧对水稻生长及其生理参数的影响[J].中国稀土学报,2003,21(1):71.Xie Z B,Zhu J G,Zhu H Y,Zhang Y L,Gao R,Zeng Q,Cao Z H.Effect of lanthanum on rice growth and physiological parameters with split-root nutrient solution method[J].Journal of the Chinese Society of Rare Earths,2003,21(1):71.
[11]吴红英,韦大器,陈丽文,蔡林,陈乃明.La(NO_3)_3和LaCl_3对桉树组培苗及扦插苗生根的影响研究[J].林业实用技术,2009,2:10.Wu H Y,Wei D Q,Chen L W,Cai L,Chen N M.Effect of La(NO_3)_3 and LaCl_3 on rooting of eucalyptus seedlings and cuttings[J].Practical Forestry Technology,2009,2:10.
[12]李际红,孟凡志,刘海燕,张义群.稀土及多胺对欧石楠瓶苗生根的效应[J].山东农业大学学报(自然科学版),2012,43(2):169.Li J H,Meng F Z,Liu H Y,Zhang Y Q.The effect of rare-earth and polyamines on rooting of the of Calluna Vulgaris[J].Journal of Shandong Agricultural University(Natural Science Edition),2012,43(2):169.
[13]Xu J,Wang Y Z,Luo J L.Stimulation of shoot proliferation by lanthanum nitrate and plant regeneration of Jojoba plantlet in vitro[J].Nat.Prod.Res.Dev.,2006,16(6):564.
[14]莫昭展,曹福亮,欧祖兰.硝酸镧对流苏石斛试管苗生长的影响[J].南京林业大学学报(自然科学版),2009,33(4):158.Mo Z Z,Cao F L,Ou Z L.Effects of lanthanum nitrate on growth of Dendrobium fimbriatum tube seedling[J].Journal of Nanjing Forestry University(Natural Science.Edition),2009,33(4):158.
[15]Guo B,Xu L L,Guan Z J,Wei Y H.Effect of lanthanum on rooting of in vitro regenerated shoots of Saussurea involucrata Kar.et Kir[J].Biol.Trace Elem.Res.,2012,147(1-3):334.
[16]江华波,王盛锋,高丽丽,陈磊,黄金生,乙引,汪洪.LaCl_3对硝态氮供应下小麦根系生长的影响[J].中国稀土学报,2013,31(4):495.Jiang H B,Wang S F,Gao L L,Chen L,Huang J S,Yi Y,Wang H.Effects of lanthanum chloride addition on root growth of wheat plants grown under different concentrations of nitrate supply[J].Journal of the Chinese Society of Rare Earths,2013,31(4):495.
[17]Liu D,Wang X,Zhang X,Gao Z.Effects of lanthanum on growth and accumulation in roots of rice seedlings[J].Plant Soil Environ.,2013,59(5):196.
[18]张海霞,陈明辉,张然,朱卫华.浅谈稀土在农业生产中的应用[J].农业装备技术,2007,33(4):17.Zhang H X,Cheng M H,Zhang R,Zhu W H.Simple talking about application of rare-earth in agricultural aspects[J].Agricultural Equipment and Technology,2007,33(4):17.
[19]王静,安忠民,冯学赞.硝酸斓对马铃薯试管苗生长的影响[J].植物生理学通讯,2003,39(1):25.Wang J,An Z M,Feng X Z.The effects of lanthanum nitrate on potato plantlets in vitro[J].Plant Physiology Journal,2003,39(1):25.
[20]江铃,周燮,王章荣.硝酸镧对马尾松苗的生长及内源激素含量的影响[J].南京农业大学学报,1998,21(2):1.Jiang L,Zhou X,Wang Z R.Effect of La(NO_3)_3 on growth and levels of endogenous hormones in masson pine(Pinus massoniana Lamb.)seedlings[J].Journal of Nanjing Agricultural University,1998,21(2):1.
[21]宋卫平,顾福根,王纪军,刘佳佳,溪群.LaCl_3对欧洲李(Prunus domestica)GF_(43)试管苗根系生长及衰老作用的影响[J].中国稀土学报,2004,22(5):687.Song W P,Gu F G,Wang J J,Liu J J,Xi Q.Effects of lanthanum on root growth and senescence of GF_(43)(Prunus domestica)plantlet in vitro[J].Journal of the Chinese Society of Rare Earths,2004,22(5):687.
[22]陈元林,陈振德.稀土对植物体抗逆性影响的研究进展[C].山东省植物生物学研究进展学术年会论文集,山东威海,2006,87.Chen Y L,Chen Z D.Research progress of effect of rare earth on plant resistance[J].Research on Shandong Province Plant Biology Symposium.Weihai,Shandong,2006,87.
[23]孙占育,郭春会,孙志强,曹斌.长柄扁桃试管苗诱导生根的初步研究[J].西北林学院学报,2010,25(3):87.Sun Z Y,Guo C H,Sun Z Q,Cao B.A preliminary study on in vitro rooting of Amygdalus Pedunculata[J].Journal of Northwest Forestry University,2010,25(3):87.
[2]许新桥,褚建民.长柄扁桃产业发展潜势分析及问题对策研究[J].林业资源管理,2013,2(1):22.Xu X Q,Chu J M.Analysis on development potential and countermeasures of Amygdalus pedunculata industry[J].Forest Resources Management,2013,2(1):22.
[3]Chu J,Xu X,Zhang Y.Production and properties of biodiesel produced from Amygdalus pedunculata Pall.[J].Bioresource technol.,2013,4(134):374.
[4]孙占育.长柄扁桃茎尖离体培养研究[J].安徽农业科学,2010,38(19):9988.Sun Z Y.Study on micropropagation of Amygdalus pedunculata Pall.Shoot-tips in vitro[J].Journal of Anhui Agricultural Sciences,2010,38(19):9988.
[5]巩东辉,李国龙,张少英,陈涛.外源稀土La~(3+)对鄂尔多斯高原碱湖钝顶节旋藻生长及生理特性的影响[J].中国稀土学报,2011,29(4):504.Gong D H,Li G L,Zhang S Y,Chen T.Effects of external rare earth La~(3+)on growth and physiological property of Arthrospira in alkaline lake of Erdos plateau[J].Journal of the Chinese Society of Rare Earths,2011,29(4):504.
[6]张子学,胡红德,胡能兵,张从宇,崔广荣.稀土对NaCl胁迫下矮牵牛幼苗生长抑制的缓解效应[J].中国稀土学报,2010,28(6):760.Zhang Z X,Hu H D,Hu N B,Zhang C Y,Cui G R.Mitigation effect of rare-earth on seeding growth and rooting of Petunia under NaCl Stress[J].Journal of the Chinese Society of Rare Earths,2010,28(6):760.
[7]李树蕾,陈曦,聂毓秀,黄可欣.长期口服硝酸稀土对大鼠腺垂体生长激素细胞的影响[J].中国稀土学报,2003,21(6):691.Li S L,Chen X,Nie Y X,Huang K X.Effect of rare earth nitrate administrated long-term orally on rat somatotroph in adenohypophysis[J].Journal of the Chinese Society of Rare Earths,2003,21(6):691.
[8]陈远孟,白厚义,李杨瑞.镧在主要农作物上的应用及其生理生化作用[J].广西农业科学,2003,(6):15.Chen Y M,Bai H Y,Li Y R.Application and physiological and biochemical effects of lanthanum on major crops[J].Guangxi Agricultural Sciences,2003,(6):15.
[9]Hu Z,Richter H,Sparovek G,Schnug E.Physiological and biochemical effects of rare earth elements on plants and their agricultural significance:a review[J].J.Plant Nutr.,2004,(27):183.
[10]谢祖彬,朱建国,褚海燕,张雅丽,高人,曾青,曹志洪.分根法研究镧对水稻生长及其生理参数的影响[J].中国稀土学报,2003,21(1):71.Xie Z B,Zhu J G,Zhu H Y,Zhang Y L,Gao R,Zeng Q,Cao Z H.Effect of lanthanum on rice growth and physiological parameters with split-root nutrient solution method[J].Journal of the Chinese Society of Rare Earths,2003,21(1):71.
[11]吴红英,韦大器,陈丽文,蔡林,陈乃明.La(NO_3)_3和LaCl_3对桉树组培苗及扦插苗生根的影响研究[J].林业实用技术,2009,2:10.Wu H Y,Wei D Q,Chen L W,Cai L,Chen N M.Effect of La(NO_3)_3 and LaCl_3 on rooting of eucalyptus seedlings and cuttings[J].Practical Forestry Technology,2009,2:10.
[12]李际红,孟凡志,刘海燕,张义群.稀土及多胺对欧石楠瓶苗生根的效应[J].山东农业大学学报(自然科学版),2012,43(2):169.Li J H,Meng F Z,Liu H Y,Zhang Y Q.The effect of rare-earth and polyamines on rooting of the of Calluna Vulgaris[J].Journal of Shandong Agricultural University(Natural Science Edition),2012,43(2):169.
[13]Xu J,Wang Y Z,Luo J L.Stimulation of shoot proliferation by lanthanum nitrate and plant regeneration of Jojoba plantlet in vitro[J].Nat.Prod.Res.Dev.,2006,16(6):564.
[14]莫昭展,曹福亮,欧祖兰.硝酸镧对流苏石斛试管苗生长的影响[J].南京林业大学学报(自然科学版),2009,33(4):158.Mo Z Z,Cao F L,Ou Z L.Effects of lanthanum nitrate on growth of Dendrobium fimbriatum tube seedling[J].Journal of Nanjing Forestry University(Natural Science.Edition),2009,33(4):158.
[15]Guo B,Xu L L,Guan Z J,Wei Y H.Effect of lanthanum on rooting of in vitro regenerated shoots of Saussurea involucrata Kar.et Kir[J].Biol.Trace Elem.Res.,2012,147(1-3):334.
[16]江华波,王盛锋,高丽丽,陈磊,黄金生,乙引,汪洪.LaCl_3对硝态氮供应下小麦根系生长的影响[J].中国稀土学报,2013,31(4):495.Jiang H B,Wang S F,Gao L L,Chen L,Huang J S,Yi Y,Wang H.Effects of lanthanum chloride addition on root growth of wheat plants grown under different concentrations of nitrate supply[J].Journal of the Chinese Society of Rare Earths,2013,31(4):495.
[17]Liu D,Wang X,Zhang X,Gao Z.Effects of lanthanum on growth and accumulation in roots of rice seedlings[J].Plant Soil Environ.,2013,59(5):196.
[18]张海霞,陈明辉,张然,朱卫华.浅谈稀土在农业生产中的应用[J].农业装备技术,2007,33(4):17.Zhang H X,Cheng M H,Zhang R,Zhu W H.Simple talking about application of rare-earth in agricultural aspects[J].Agricultural Equipment and Technology,2007,33(4):17.
[19]王静,安忠民,冯学赞.硝酸斓对马铃薯试管苗生长的影响[J].植物生理学通讯,2003,39(1):25.Wang J,An Z M,Feng X Z.The effects of lanthanum nitrate on potato plantlets in vitro[J].Plant Physiology Journal,2003,39(1):25.
[20]江铃,周燮,王章荣.硝酸镧对马尾松苗的生长及内源激素含量的影响[J].南京农业大学学报,1998,21(2):1.Jiang L,Zhou X,Wang Z R.Effect of La(NO_3)_3 on growth and levels of endogenous hormones in masson pine(Pinus massoniana Lamb.)seedlings[J].Journal of Nanjing Agricultural University,1998,21(2):1.
[21]宋卫平,顾福根,王纪军,刘佳佳,溪群.LaCl_3对欧洲李(Prunus domestica)GF_(43)试管苗根系生长及衰老作用的影响[J].中国稀土学报,2004,22(5):687.Song W P,Gu F G,Wang J J,Liu J J,Xi Q.Effects of lanthanum on root growth and senescence of GF_(43)(Prunus domestica)plantlet in vitro[J].Journal of the Chinese Society of Rare Earths,2004,22(5):687.
[22]陈元林,陈振德.稀土对植物体抗逆性影响的研究进展[C].山东省植物生物学研究进展学术年会论文集,山东威海,2006,87.Chen Y L,Chen Z D.Research progress of effect of rare earth on plant resistance[J].Research on Shandong Province Plant Biology Symposium.Weihai,Shandong,2006,87.
[23]孙占育,郭春会,孙志强,曹斌.长柄扁桃试管苗诱导生根的初步研究[J].西北林学院学报,2010,25(3):87.Sun Z Y,Guo C H,Sun Z Q,Cao B.A preliminary study on in vitro rooting of Amygdalus Pedunculata[J].Journal of Northwest Forestry University,2010,25(3):87.