基于体外模型对硫酸多糖体内活性机制的研究
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- 英文论文题名：The mechanism of action of sulfated polysaccharide in vivo based on in vitro models
- 论文作者：马娜 ; 宋爽 ; 徐鑫 ; 张豹 ; 刘斌 ; 艾春青
- 英文论文作者：Ma Na ; Song Shuang ; Xu Xin ; Zhang Bao ; Liu Bing ; Ai Chunqing ; School of food science and technology ; Dalian Polytechnic University National Engineering Research Center of Seafood
- 年：2016
- 作者机构：大连工业大学食品学院国家海洋食品工程技术研究中心;
- 论文关键词：硫酸多糖 ; 动物实验 ; 体外模拟消化 ; 肠道菌群
- 英文论文关键词：polysaccharide sulfate ; animal experiment ; in vitro digestion ; intestinal flora
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.3
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：117k
- 原文格式：O
- 会议级别：全国
摘要
硫酸多糖AGSP是自鲍鱼内脏中分离的一类酸性杂多糖,已被证实在免疫调节、抗氧化、抗凝血等方面都具有较好的生物活性,但目前对其体内的作用机制尚不清晰。本研究拟通过对动物实验、体外模拟消化、肠道菌群发酵等方面的研究,初步阐释AGSP的体内活性机制。基于高效凝胶色谱、DNS比色法和PMP衍生化法对消化液(唾液、胃液、胃肠液)处理后AGSP的分析,结果表明机体消化系统对AGSP的分子结构未有明显影响。为进一步探究AGSP的作用机制,本研究对肠道微生物与AGSP的相互作用进行了分析。体外发酵实验表明AGSP可以通过促进双歧杆菌、乳酸杆菌、拟杆菌等菌群的增殖,调节短链脂肪酸的表达,影响肠道菌群的微生态结构。对发酵上清中多糖组分的分析表明肠道菌群可以利用AGSP,并将其降解成小分子量的寡糖片段,进而促进其被机体吸收。结论:本研究通过模拟AGSP在体内的消化过程,明确肠道菌群与AGSP的相互作用是AGSP体内发挥其活性作用的重要环节,为深入探究活性多糖的分子作用机制奠定了理论基础。
Sulfated polysaccharide AGSP,extracted from abalonesviscera,is a kind of acidic hetero-polysaccharide,and considered to play important roles in immune regulation,antioxidant,and antithrombotic,butitsexact mechanisms involved are still not unclear or controversial.In this study,the action mechanisms of AGSP in vivowere studied via in vitro digestion models involving the mouth,gastric,small and large intestine conditions.The results of HPGPC,DNS colorimetric method and HPLC-MS/MS showed thatthe molecular structure of AGSP was not significantly affected by the body's digestion systems.To further explore the in vivo mechanisms of AGSP,the interaction between intestinal microorganism and AGSP was evaluated by analyzing intestinal microbiota and the utilization of polysaccharide.Itindicated that AGSP couldsignificantly affectintestinal microbiota composition by stimulating the proliferation of bifidobacterium,lactobacillus and bacteroides,and the expression of short chain fatty acid.Moreover,the composition analysis of polysaccharide in the fermentation fluid indicated that AGSP could be degraded intothe oligosaccharide fragment,and thus has a chance to be absorbed by the body.Conclusion:Based on the study of in vitro digestion,this study demonstrated that the interaction between intestinal microbiota and AGSP played a critical role in its biological active effect,which would provide an important theoretical foundation for the study of action mechanisms of polysaccharide in vivo.
Sulfated polysaccharide AGSP,extracted from abalonesviscera,is a kind of acidic hetero-polysaccharide,and considered to play important roles in immune regulation,antioxidant,and antithrombotic,butitsexact mechanisms involved are still not unclear or controversial.In this study,the action mechanisms of AGSP in vivowere studied via in vitro digestion models involving the mouth,gastric,small and large intestine conditions.The results of HPGPC,DNS colorimetric method and HPLC-MS/MS showed thatthe molecular structure of AGSP was not significantly affected by the body's digestion systems.To further explore the in vivo mechanisms of AGSP,the interaction between intestinal microorganism and AGSP was evaluated by analyzing intestinal microbiota and the utilization of polysaccharide.Itindicated that AGSP couldsignificantly affectintestinal microbiota composition by stimulating the proliferation of bifidobacterium,lactobacillus and bacteroides,and the expression of short chain fatty acid.Moreover,the composition analysis of polysaccharide in the fermentation fluid indicated that AGSP could be degraded intothe oligosaccharide fragment,and thus has a chance to be absorbed by the body.Conclusion:Based on the study of in vitro digestion,this study demonstrated that the interaction between intestinal microbiota and AGSP played a critical role in its biological active effect,which would provide an important theoretical foundation for the study of action mechanisms of polysaccharide in vivo.
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