剂量-效应图法研究二元含汞重金属混合物对费氏弧菌的联合毒性
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摘要
以费氏弧菌(Vibrio fischeri)作为毒性测试物种,测定了Hg~(2+)、Pb~(2+)、Cu~(2+)、Cd~(2+)、Zn~(2+)、Cr~(6+)对费氏弧菌的单一毒性;采用剂量-效应图法研究了Hg~(2+)/Pb~(2+)、Hg~(2+)/Cr~(6+)、Hg~(2+)/Cu~(2+)、Hg~(2+)/Zn~(2+)、Hg~(2+)/Cd~(2+)五种二元混合体系的联合毒性作用。结果表明,Hg~(2+)、Pb~(2+)、Cu~(2+)、Cd~(2+)、Zn~(2+)、Cr~(6+)单独存在时对费氏弧菌的EC_(50)分别为0.045、0.181、0.300、0.117、0.614、23.0 mg/L,毒性大小依次为Hg~(2+)>Cd~(2+)>Pb~(2+)>Cu~(2+)>Zn~(2+)>Cr~(6+)。而与单一重金属相比,它们的二元混合物对费氏弧菌的生物毒性效应和强度完全不同。Hg~(2+)与Pb~(2+)、Hg~(2+)与Cr~(6+)二元混合体系表现为拮抗作用,Hg~(2+)与Cu~(2+)、Hg~(2+)与Zn~(2+)均表现为协同作用,Hg~(2+)与Cd~(2+)表现为加和作用。
引文
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[16]OTITOLOJU A A.Evaluation of the joint-action toxicity of binary mixtures of heavy metals against the mangrove periwinkle Tympanotomy fuscatus var radula(L)[J]?Ecotoxicology and Environmental Safety,2002,53(3).404-415.
[2]THOMULK K W,MCGEE D J,LANGE J H.Use of the bioluminescent bacterium Photobacterium phosphoreum to detect potentially biohazardons materials in water[J].Bulletin of Environmental Contaminant Toxicology,1993,51(4):538—544.
[3]江敏,顾国维,李泳梅.6种含氮杂环化合物对发光细菌的毒性研究[J].上海环境科学,2003,22(12):931-935.
[4]于景丽,刘芳,张国辉,等.发光菌法研究镉离子、草甘膦和啶虫脒的联合毒性[J].安全与环境学报,2010,10(2):1—4.
[5]高继军,张力平,马梅.应用淡水发光细菌研究二元重金属混合物的联合毒性[J].上海环境科学,2003,22(11):772-775.
[6]吴淑杭,周德平,徐亚同,等.重金属汞、镉和铬对明亮发光杆菌的生物毒性[J].农业环境科学学报,2011,30(12):2446—2450.
[7]刘清,马梅,童中华,等.Cu,Zn,Cd,Hg对青海弧菌(Q67菌株)联合毒性作用的研究[J].中国环境科学,1997,17(4):301—303.
[8]于晓丽,李秀珍等.用发光菌评价油田采油污水综合毒性[J],石油与天然气化工,2002,31(2):101—104
[9]孟庆俊,肖昕.不同方法对联合毒性作用的评价[J].污染防治技术,2004,17(1):33—35.
[10]何勇田,熊先哲.复合污染研究进展[J].环境科学,1994,15(6):79—83.
[11]Marking L L,Dawson V K.Method of assessment of toxicity or efficacy of mixtures chemicals[J].Investigation in Fish Control,1975,67(1):1-8.
[12]郑振华,周培疆,吴振斌.复合污染研究的新进展[J].应用生态学报,2001,12(3):469—473.
[13]WARNE M S J,HAWKER D W.The number of components in a mixture determines whether synergistic and antagonistic or additive toxicity predominate:The funnel hypothesis[J].Ecotoxicology and Environmental Safety,1995,31(1):23-28.
[14]PAPE-LYNDSTROM P A,LYDY M J.Synergistic toxicity of atrazine and organophosphate insecticides contravenes the response addition mixture mode[J].Environmental Toxicology and Chemistry,1997,16(11):2415-2420.
[15]FULLADOSA E.Study on the toxicity of binary equitoxic mixtures of metals using the luminescent bacteria Vibrio fischeri as a biological target[J].Chemosphere,2005,58(4):551-557.
[16]OTITOLOJU A A.Evaluation of the joint-action toxicity of binary mixtures of heavy metals against the mangrove periwinkle Tympanotomy fuscatus var radula(L)[J]?Ecotoxicology and Environmental Safety,2002,53(3).404-415.