应用小分子氯喹提升黄颡鱼精子质量的研究
详细信息 查看官网全文
- 英文论文题名：The effect and molecular mechanism of chloroquine on sperm motility and fertilizing ability in yellow catfish
- 论文作者：张晋 ; 谢彬月 ; 刘英杰 ; 梅洁
- 英文论文作者：ZHANG Jin ; XIE Binyue ; LIU Yingjie ; MEI Jie ; Key Laboratory of Freshwater Animal Breeding ; Huazhong Agricultural University
- 年：2016
- 作者机构：农业部淡水生物繁育重点实验室水产学院华中农业大学;
- 论文关键词：氯喹 ; 精子活力 ; 受精率 ; 细胞自噬 ; toll-like受体
- 英文论文关键词：Chloroquine ; sperm motility ; fertilizing ability ; autophagy ; toll-like receptor
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：537k
- 原文格式：D
- 会议级别：全国
摘要
在鱼类中,精子活力是影响雄性生殖非常关键的一种因子。氯喹,是一种细胞自噬抑制剂,曾被报道过对哺乳动物的精子质量有影响。本文主要研究了氯喹对黄颡鱼精子活力和受精率的影响。我们对黄颡鱼进行了不同浓度的氯喹胸腔注射,结果显示氯喹在抑制细胞自噬的同时,显著促进了精子活力。随后,我们对其各种精子活力参数进行了统计分析,相比对照组,氯喹处理组显著促进了精子的直线速度(VSL),曲线速度(VCL),平均路径速度(VAP)。同时根据结果显示,注射1μM/g体重的氯喹是促进黄颡鱼受精率的最佳浓度。通过对氯喹处理组和对照组精巢进行转录组分析来揭示氯喹对精子活力影响的调控机制。GO分析指出氯喹处理不仅抑制了细胞自噬通路,同时还显著降低了toll-like受体信号通路,说明了在生殖和免疫之间可能存在一种平衡。KEGG分析指出许多信号通路存在上调现象,包括与精子生成和成熟相关的PI3K-AKT信号通路。最后,我们的结果说明了氯喹可以作为一种促进黄颡鱼的精子活力的药物,可以应用到鱼类生产中。
Sperm motility is a critical determinant of male fertility in vertebrates including fish species. Chloroquine, an autophagy inhibitor, has been reported to have influence on sperm quality of mammalians. The objectives of this study were to determine the effect of chloroquine on sperm motility and fertilizing efficiency in yellow catfish. Gradient doses of chloroquine were intraperitoneally injected into yellow catfish that resulted in an increasing expression level of LC3-II and inhibition of autophagy. Subsequently, various sperm parameters were assessed with the computer-assisted sperm analysis system. Chloroquine injection significantly increased straight line velocity, curvilinear velocity and average path velocity of sperm compared with the control. Consequently, 1μM chloroquine/g body weight was revealed to be an optimal dose for in vivo treatment, which led to elevation of fertility rate. Transcriptome analysis were conducted to figure out the possible molecular mechanism of chloroquine to regulate sperm motility.Interestingly, GO analysis indicated that chloroquine treatment significantly reduced toll-like receptor signalling pathway, suggesting a possible trade-off between male reproduction and immunity.
Sperm motility is a critical determinant of male fertility in vertebrates including fish species. Chloroquine, an autophagy inhibitor, has been reported to have influence on sperm quality of mammalians. The objectives of this study were to determine the effect of chloroquine on sperm motility and fertilizing efficiency in yellow catfish. Gradient doses of chloroquine were intraperitoneally injected into yellow catfish that resulted in an increasing expression level of LC3-II and inhibition of autophagy. Subsequently, various sperm parameters were assessed with the computer-assisted sperm analysis system. Chloroquine injection significantly increased straight line velocity, curvilinear velocity and average path velocity of sperm compared with the control. Consequently, 1μM chloroquine/g body weight was revealed to be an optimal dose for in vivo treatment, which led to elevation of fertility rate. Transcriptome analysis were conducted to figure out the possible molecular mechanism of chloroquine to regulate sperm motility.Interestingly, GO analysis indicated that chloroquine treatment significantly reduced toll-like receptor signalling pathway, suggesting a possible trade-off between male reproduction and immunity.
引文