HPLC法测定麻黄鱼腥草散中黄芩苷、穿心莲内酯、脱水穿心莲内酯的含量
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- 英文论文题名：HPLC determination of baicalin in Ma Huang Yu Xing Cao powder
- 论文作者：邱天宝 ; 吴志明 ; 周红霞 ; 吴宁鹏 ; 韩立 ; 董晓盈
- 英文论文作者：QIU Tian-bao ; Wu Zhiming ; Zhou Hongxia ; Wu Ningpeng ; Han Li ; Dong Xiaoying ; Henan Institute of Veterinary Drug and feed Control ; Henan Houyi Industrial group Co ; Ltd
- 年：2016
- 作者机构：河南省兽药饲料监察所;河南后羿实业集团有限公司;
- 论文关键词：麻黄鱼腥草散 ; 黄芩苷 ; 穿心莲内酯 ; 脱水穿心莲内酯
- 英文论文关键词：Ma Huang Yu Xing Cao powder ; baicalin ; andrographolide ; dehyandrographolide
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：S853.7;O657.72
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：7
- 文件大小：861k
- 原文格式：O
- 会议级别：全国
摘要
建立HPLC法测定麻黄鱼腥草散中黄芩苷、穿心莲内酯、脱水穿心莲内酯含量的方法。色谱柱为Waters XBrige~(TM)C_(18),4.6×150mm,5μm;黄芩苷以甲醇-0.2%磷酸溶液为流动相梯度洗脱;流速:1.0mL/min;柱温:30℃:穿心莲内酯、脱水穿心莲以甲醇-水(50:50)为流动相;流速:1.0mL/min柱温:30℃;检测波长:黄芩苷检测波长276nm,穿心莲内酯检测波长225 nm,脱水穿心莲内酯检测波长为254nm。黄芩苷的线性范围为0.0798~0.7975μg,R=1.0000,平均回收率为94.3%(RSD=0.8%)。穿心莲内酯的线性范围为0.0109~0.2175μg,R=1.0000,平均回收率为92.9%(RSD=0.7%);脱水穿心莲内酯的线性范围为0.0105~0.2105μg,R=0.9999,平均回收率为92.4%(RSD=0.3%)。本方法快速、简便、准确,能有效测定麻黄鱼腥草散中黄芩苷、穿心莲内酯、脱水穿心莲的含量。
To establish an HPLC method for determination of baicalin,andrographolide and dehyandrographolideinMa Huang Yu Xing Cao powder.The baicalin was analyzed on an XBrige~(TM) C_(18) column(4.6 mm×250 mm,5 μm) using ultrapure methanol-0.2%phosphoric acid solution as the mobile phase.The flow rate was 1.0 mL/min.The column temperature was 30 ℃.The andrographolide and dehyandrographolide were analyzed on an XBrige~(TM) C_(18) column(4.6 mm×250mm,5 μm) using methanol-ultrapure water(50:50) as the mobile phase.The flow rate was 1.0 mL/min.The column temperature was 30℃.The detection wavelengthsofBaicalinwas set at 276 nm.The calibration curve was linear in the ranges of 0.0798~0.7975 μg(R = 1.0000) for baicalin.The recovery of baicalin was94.3%with RSD of 0.8%.Andrographolide and dehyandrographolide were detected at 225 nm and 254 nm.The calibration curves were linear in the ranges of 0.0109~0.2175 μg(R = 1.0000) and0.0105~0.2105μg(0.9999) for andrographolide and dehyandrographolide.The average recoveries were 92.9%for andrographolide(RSD=0.7%,n=6) and 92.4%for dehyandrographolide(RSD=0.3%,n=6),respectively.This method is rapid,simple and accurate,which can be used for the determination of baicalin,andrographolide and dehyandrographolidein Ma Huang Yu Xing Cao powder.
To establish an HPLC method for determination of baicalin,andrographolide and dehyandrographolideinMa Huang Yu Xing Cao powder.The baicalin was analyzed on an XBrige~(TM) C_(18) column(4.6 mm×250 mm,5 μm) using ultrapure methanol-0.2%phosphoric acid solution as the mobile phase.The flow rate was 1.0 mL/min.The column temperature was 30 ℃.The andrographolide and dehyandrographolide were analyzed on an XBrige~(TM) C_(18) column(4.6 mm×250mm,5 μm) using methanol-ultrapure water(50:50) as the mobile phase.The flow rate was 1.0 mL/min.The column temperature was 30℃.The detection wavelengthsofBaicalinwas set at 276 nm.The calibration curve was linear in the ranges of 0.0798~0.7975 μg(R = 1.0000) for baicalin.The recovery of baicalin was94.3%with RSD of 0.8%.Andrographolide and dehyandrographolide were detected at 225 nm and 254 nm.The calibration curves were linear in the ranges of 0.0109~0.2175 μg(R = 1.0000) and0.0105~0.2105μg(0.9999) for andrographolide and dehyandrographolide.The average recoveries were 92.9%for andrographolide(RSD=0.7%,n=6) and 92.4%for dehyandrographolide(RSD=0.3%,n=6),respectively.This method is rapid,simple and accurate,which can be used for the determination of baicalin,andrographolide and dehyandrographolidein Ma Huang Yu Xing Cao powder.
引文
[1]中国兽药典委员会.中华人民共和国兽药典2010年版二部[S].
[2]肖崇厚.中药化学[M].北京:人民卫生出版社,1984.
[2]郭蓓.穿心莲的研究及临床开发[J].药学进展.2004(12)
[3]姜永忠,彭永久.穿心莲的兽医临床应用及内酯提取[J].兽药与饲料添加剂.2001(01)
[4]李建华,王力生,邹节明.水提工艺中黄芩内源酶降解黄芩苷的研究[J].中草药:40(3)397-400.
[5]聂凌云,罗兴平.穿心莲提取工艺的研究及热稳定性考察[J].解放军药学学报,2005,21(1):32-33,86.
[6]张红星.穿心莲叶含量对药材质量影响的研究[J].中国现代药物应用,2015 9(5):265-266.
[2]肖崇厚.中药化学[M].北京:人民卫生出版社,1984.
[2]郭蓓.穿心莲的研究及临床开发[J].药学进展.2004(12)
[3]姜永忠,彭永久.穿心莲的兽医临床应用及内酯提取[J].兽药与饲料添加剂.2001(01)
[4]李建华,王力生,邹节明.水提工艺中黄芩内源酶降解黄芩苷的研究[J].中草药:40(3)397-400.
[5]聂凌云,罗兴平.穿心莲提取工艺的研究及热稳定性考察[J].解放军药学学报,2005,21(1):32-33,86.
[6]张红星.穿心莲叶含量对药材质量影响的研究[J].中国现代药物应用,2015 9(5):265-266.