藏猪与雅南猪的IGF-I基因的克隆与序列分析
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- 英文论文题名：Cloning and sequence analysis of IGF-I gene of Tibetan porcine and Yanan porcine
- 论文作者：李伟 ; 张飞燕 ; 宋婷婷 ; 谷笑笑 ; 刘红露 ; 潘大敏 ; 韩书饼 ; 潘康成
- 英文论文作者：Wei Li ; Feiyan Zhang ; Tingting Song ; Xiaoxiao Gu ; Honglu Liu ; Damin Pan ; Shubing Han ; Kangcheng Pan ; Animal Microecology Institute ; College of Veterinary Medicine ; Sichuan Agricultural University ; Key Laboratory of Animal Disease and Human Health of Sichuan Province
- 年：2016
- 作者机构：四川农业大学动物医学院动物微生态研究中心;动物疫病与人类健康四川省重点实验室;
- 论文关键词：藏猪 ; 雅南猪 ; IGF-1基因 ; 克隆 ; 序列分析
- 英文论文关键词：Tibetan porcine ; Yanan porcine ; porcine of IGF-I Gene ; Clone ; sequence analysis
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：S828;Q78
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：74k
- 原文格式：O
- 会议级别：全国
摘要
从藏猪和雅南猪肝脏克隆出胰岛素样生长因子(IGF-I)基因,并对其基因序列进行分析。以GenBank中猪IGF-I基因序列设计引物,应用RT-PCR技术从藏猪和雅南猪肝脏克隆到IGF-1基因,分别将藏猪、雅南猪IGF-I基因克隆到PMD19-T载体上,构建重组质粒pMD19-T-IGF-I,PCR鉴定、测序及分析。从藏猪和雅南猪肝脏c-DNA中克隆得到的基因片段,经PCR鉴定及测序分析,表明为猪IGF-I基因,其基因大小均为612bp:该基因包含完整的开放阅读框462个碱基,编码153个氨基酸;[结论]所测的藏猪和雅南猪基因序列与己在NCBI的猪(DQ121132.)的IGF-I基因高度同源,比对序列发现,藏猪的同源性为100%,雅南猪的为99%;藏猪IGF-I与雅南猪IGF-I基因序列的同源性为99%;雅南猪在258bp处发生A-G的突变,但其编码的氨基酸未发生改变。
The study aimed at cloning and analyzing the insulin-like growth factor-1(IGF-1) gene from liver of Tibetan porcine and Yannan porcine.The total RNA was extracted by using Trizol from the liver of Tibetan porcine and Yannan porcine and used as template to amplify IGF-1 gene by RT-PCR,A pair of primers was designed by sus scrofa IGF-1 gene sequence from Genbank.The cDNA was connected to the pMD19-T vector,and the recombinant plasmid pMD-19T-IGF-I Was constructed and sequenced.The Tibetan porcine and Yannan porcine IGF-1 gene were 612 bp,Then proved to be Tibetan porcine and Yanan porcine with PCR identification and sequence identification.The sequencing result indicated that the IGF-1 gene consisted of 612 nucleotides,Which contains the complete open reading frame of 462 bp,encods 153 amino acids.It showed there was high homology between the Tibetan porcine and Yanan porcine IGF-I,which was an identity of 99%.the two IGF-I compared with sus scrofa(DQ121132.) sequence published in the NCBI,Which were an identidy of 100%and 99%.we found that the mutation in the 258 bp,occurred at A-G mutation.and the corresponding amino acids not changed.
The study aimed at cloning and analyzing the insulin-like growth factor-1(IGF-1) gene from liver of Tibetan porcine and Yannan porcine.The total RNA was extracted by using Trizol from the liver of Tibetan porcine and Yannan porcine and used as template to amplify IGF-1 gene by RT-PCR,A pair of primers was designed by sus scrofa IGF-1 gene sequence from Genbank.The cDNA was connected to the pMD19-T vector,and the recombinant plasmid pMD-19T-IGF-I Was constructed and sequenced.The Tibetan porcine and Yannan porcine IGF-1 gene were 612 bp,Then proved to be Tibetan porcine and Yanan porcine with PCR identification and sequence identification.The sequencing result indicated that the IGF-1 gene consisted of 612 nucleotides,Which contains the complete open reading frame of 462 bp,encods 153 amino acids.It showed there was high homology between the Tibetan porcine and Yanan porcine IGF-I,which was an identity of 99%.the two IGF-I compared with sus scrofa(DQ121132.) sequence published in the NCBI,Which were an identidy of 100%and 99%.we found that the mutation in the 258 bp,occurred at A-G mutation.and the corresponding amino acids not changed.
引文
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