表面活性剂和微波辅助预处理柑橘皮提高Aspergillus japonicus PJ01产胞外酶
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- 英文论文题名：Utilization of surfactants and microwave-assisted pretreatedorange peel for the extracellular enzymes production by Aspergillus japonicus PJ01
- 论文作者：李培骏 ; 夏金兰 ; 单杨
- 英文论文作者：Li Peijun ; Xia Jinlan ; Shan Yang ; College of Chemistry & Bioengineering ; Guilin University of Technology ; School of Minerals Processing and Bioengineering ; Central South University ; Hunan Agriculture Product Processing Institute ; Hunan Academy of Agricultural Sciences
- 年：2016
- 作者机构：桂林理工大学化学与生物工程学院;中南大学资源加工与生物工程学院;湖南省农科院农产品加工研究所;
- 论文关键词：微波预处理 ; 表面活性剂 ; 柑橘皮 ; 产酶 ; 日本曲霉
- 英文论文关键词：microwave pretreatment ; surfactants ; orange peel ; enzyme production ; aspergillusjaponicus
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TQ925
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：108k
- 原文格式：O
- 会议级别：全国
摘要
近年来低成本的农业废弃物越来越多的用于发酵制备复合酶,研究证实通过物理化学方法预处理发酵底物可以提高其产酶效率。本论文采用表面活性剂和微波预处理发酵底物橘皮粉(OPP),探讨了预处理方法对日本曲霉Aspergillus japonicus PJ01液态发酵产胞外酶果胶酶、纤维素酶和木聚糖酶的影响。结果表明当微波预处理条件为功率630W、时间9min以及液固比5时,外切果胶酶、羧甲基纤维素酶(CMCase)、木聚糖酶和滤纸酶(FPase)分别增长了11.8、20.6、16.2和24.0%。另外,当利用上述条件处理的OPP作为发酵底物,然后在发酵液中添加浓度为3g/L的表面活性剂聚乙二醇PEG 4000,经84h发酵上述酶活分别增长40.2、30.3、40.4和40.0%。以上结果表明微波和表面活性剂预处理OPP对于A.japonicus PJ01发酵产酶具有协同增长作用。化学组成及傅里叶变换红外光谱(FTIR)分析显示微波预处理OPP导致半纤维素及精油含量的降低,扫描电镜(SEM)表明微波处理后OPP的表面变得多孔利于日本曲霉生长。本研究证实,表面活性剂PEG 4000和预处理OPP能够提高真菌发酵农业废弃物柑橘皮产酶的效率,并且具有环境友好和成本节约的优点。
In recent years,the low-cost agricultural residues have a greatpotential as substrates for industrial enzyme production,recent studies indicated thatphysical and chemical pretreatment of substrate could improve the enzyme production efficiency.In this work,the effects of surfactants and microwave pretreatment of orange peel powder(OPP) on the production of pectinase,cellulase,and xylanase by Aspergillus japonicus PJ01 insubmerged fermentation were investigated.The results showed that when OPP was pretreatedonly by microwave(630 W,9min,and liquid/solid ratio 5),the activities of exo-pectinase,carboxymethyl cellulase(CMCase),xylanase,and filter paper cellulase(FPase) wereincreasedby 11.8,20.6,16.2,and 24.0%,respectively,and when OPP was pretreated by microwave atthe same conditions cited above plus PEG4000 at the concentration of 3g/L,the activities ofthe above four enzymes were enhanced by 40.2,30.3,40.4,and 40.0%after 84-h cultivation,respectively.It is suggested a synergistic effect between microwave and surfactant treatment inenhancing the multiextracellular enzymes production by OPP fermentation of A.japonicusPJ01.Chemical composition and Fourier transform infrared spectroscopy(FTIR) analysisdisplayed that the microwavepretreatment of OPP led to the decrease of hemicellulose andessential oil contents.Scanning electron microscope(SEM) showed that OPP surface aftermicrowave pretreatment became porous and more susceptible to be invaded by A.japonicus.The results demonstrated that pretreatment of OPP by surfactant PEG 4000 and microwaveirradiation asenvironment-friendly way was cost-effective in enhancing the multienzymeproduction from agricultural waste orange peel.
In recent years,the low-cost agricultural residues have a greatpotential as substrates for industrial enzyme production,recent studies indicated thatphysical and chemical pretreatment of substrate could improve the enzyme production efficiency.In this work,the effects of surfactants and microwave pretreatment of orange peel powder(OPP) on the production of pectinase,cellulase,and xylanase by Aspergillus japonicus PJ01 insubmerged fermentation were investigated.The results showed that when OPP was pretreatedonly by microwave(630 W,9min,and liquid/solid ratio 5),the activities of exo-pectinase,carboxymethyl cellulase(CMCase),xylanase,and filter paper cellulase(FPase) wereincreasedby 11.8,20.6,16.2,and 24.0%,respectively,and when OPP was pretreated by microwave atthe same conditions cited above plus PEG4000 at the concentration of 3g/L,the activities ofthe above four enzymes were enhanced by 40.2,30.3,40.4,and 40.0%after 84-h cultivation,respectively.It is suggested a synergistic effect between microwave and surfactant treatment inenhancing the multiextracellular enzymes production by OPP fermentation of A.japonicusPJ01.Chemical composition and Fourier transform infrared spectroscopy(FTIR) analysisdisplayed that the microwavepretreatment of OPP led to the decrease of hemicellulose andessential oil contents.Scanning electron microscope(SEM) showed that OPP surface aftermicrowave pretreatment became porous and more susceptible to be invaded by A.japonicus.The results demonstrated that pretreatment of OPP by surfactant PEG 4000 and microwaveirradiation asenvironment-friendly way was cost-effective in enhancing the multienzymeproduction from agricultural waste orange peel.
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