间歇低氧大鼠心室重塑与心肌细胞凋亡和Rho激酶表达的关系
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- 英文论文题名：Expression between remodling, apoptosis and Rho kinase in myocardial cells with sleep intermittent hypoxia
- 论文作者：王实 ; 张曼
- 英文论文作者：Wang Shi ; zhang man ; Department of respiratory ; the center Hospital Affiliated to Shenyang Medical College
- 年：2012
- 作者机构：沈阳医学院附属中心医院呼吸内科;
- 论文关键词：间歇低氧 ; 心室重塑 ; 心力衰竭 ; 细胞凋亡 ; Rho激酶 ; 睡眠呼吸暂停
- 英文论文关键词：Intermittent hypoxia ; Remodling ; Heart failure ; Apoptosis ; Rho kinase ; Sleep apnea syndrome
- 会议召开时间：2012-09-29
- 会议录名称：第二届海峡两岸中医心理学睡眠医学论坛论文汇编
- 英文会议录名称：Proceedings of the 2nd Cross-Straits TCM Psychology & Sleep Medicine Forum
- 语种：中文
- 分类号：R541.6;R766
- 学会代码：YXSM
- 会议名称：第二届海峡两岸中医心理学睡眠医学论坛
- 会议地点：中国台湾台北
- 主办单位：世界中医药学会联合会中医心理学专业委员会、世界中医药学会联合会睡眠医学专业委员会
- 学会名称：世界中医药学会联合会睡眠医学专业委员会
- 页数：9
- 文件大小：639k
- 原文格式：D
- 会议级别：全国
摘要
目的观察睡眠中间歇低氧状态下大鼠心室重塑、血液动力学异常与心肌细胞凋亡和Rho激酶表达的关系,探讨间歇低氧与心室重塑和心力衰竭之间关系的分子机制。方法 3组雄性Wistar大鼠,每组8只,给予适度限制饮食量的普通饮食和每天正常运动量游泳1h,分为常氧(A组)、间歇低氧(B组)、间歇低氧+法舒地尔(C组),60d后检测血液动力学指标、心室重量、体重、形态学检测(HE染色和凋亡细胞检测)和RT-PCR查Rho激酶m RNA表达。结果与A组比较,B组血液动力学指标恶化、心室肥厚指数增加、心肌细胞排列紊乱、凋亡明显及Rho激酶表达增加。与B组比较,C组血液动力学指标改善、心室肥厚指数减小、心肌细胞排列紊乱减轻、凋亡减少及Rho激酶表达降低。结论间歇低氧与心室重塑、心力衰竭发生、发展密切相关。细胞凋亡和炎症因子Rho激酶表达增加与之密切相关。
Objective To study the relationship between remodling, hemodynamics parameters with the apoptosis and the expression of Rho kinase in myocardial cells with sleep intermittent hypoxia, and therefore to explore the molecular mechanisms of between intermittent hypoxia and remodling and heart failure.Methods Male Wistar rats were divided into 3 groups: a normal oxygen group(A group, n=8, breathing 21% O2 for 8h per day, eating limit normal diet 20g/d foreach rat, swimming 1h in 6pm), an intermittent hypoxia group(B group, n=8,breathing 10% O2 and air alterd per90 sec for 8h per day, eating limit normal diet 20g/d for each rat, swimming 1h in 6pm) and a fasudil group(C group, n=8,breathing 10% O2 and air alterd per90 sec for 8h per day, eating limit normal diet 20g/d for each rat, swimming 1h in 6pm, 20mg·kg-1·d-1 fasudil Qd, i.h.)Sixty days later, hemodynamics parameters, left ventricular weight, weight, HE staining of myocardial cells, apoptosis and Rho kinase m RNA expression were measured respectively. Results The hemodynamics parameters were deteriorated,the index of left ventricular hypertrophy was increased, myocardial cells were arranged in disorder, the apoptosis was obvious and the expression of Rho kinase m RNA was increased in B group as compared with A group. The conditions in C group were improved significantly as compared with B group. Conclusion Intermittent hypoxia as dangerous factor was associated with the occurrence and development of remodling and heart failure. The activitions of apoptosis and inflammatory cytokine-- Rho kinase may be the key factors in remodling and heart failure under sleep intermittent hypoxia.
Objective To study the relationship between remodling, hemodynamics parameters with the apoptosis and the expression of Rho kinase in myocardial cells with sleep intermittent hypoxia, and therefore to explore the molecular mechanisms of between intermittent hypoxia and remodling and heart failure.Methods Male Wistar rats were divided into 3 groups: a normal oxygen group(A group, n=8, breathing 21% O2 for 8h per day, eating limit normal diet 20g/d foreach rat, swimming 1h in 6pm), an intermittent hypoxia group(B group, n=8,breathing 10% O2 and air alterd per90 sec for 8h per day, eating limit normal diet 20g/d for each rat, swimming 1h in 6pm) and a fasudil group(C group, n=8,breathing 10% O2 and air alterd per90 sec for 8h per day, eating limit normal diet 20g/d for each rat, swimming 1h in 6pm, 20mg·kg-1·d-1 fasudil Qd, i.h.)Sixty days later, hemodynamics parameters, left ventricular weight, weight, HE staining of myocardial cells, apoptosis and Rho kinase m RNA expression were measured respectively. Results The hemodynamics parameters were deteriorated,the index of left ventricular hypertrophy was increased, myocardial cells were arranged in disorder, the apoptosis was obvious and the expression of Rho kinase m RNA was increased in B group as compared with A group. The conditions in C group were improved significantly as compared with B group. Conclusion Intermittent hypoxia as dangerous factor was associated with the occurrence and development of remodling and heart failure. The activitions of apoptosis and inflammatory cytokine-- Rho kinase may be the key factors in remodling and heart failure under sleep intermittent hypoxia.
引文
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[2]Chinese Sleep and Respiratory Diseases Association.A multicenter study of the prevalence of sleep apnea with hypertension[J]。Chinese Journal of Tuberculosis and Respiratory Disease,2007,30(12):894-897
[3]Zhao JX,Tian Y,Cao JM,et al.Effects of different exercises on hemoglobin levels in rats-an animal model of sports anemia[J].Chinese Journal of sports medicine,2004,23(4):436-440.
[4]Feng XW.The regulation of hypothalamus-pituitary-adrenal axis and growth hormone axis in experimental intermittent hypoxia rats and obstructive sleep apnea syndrome patients[D].China's full-text database dissertation of outstanding master or doctor degree(doctor degree),2005,(06).
[5]Kimoff RJ,Sforza E,Champagne V,et al.Upper airway sensation in snoring and obstructive sleep apnea[J].Am J Respair Crit Care Med,2001,164(2):250-255.
[6]Zhang NF,Zhong NS.The relationship between sleep apnea-hypopnea syndrome and the cerebrovascular disease[J].Chinese Journal of Tuberculosis and Respiratory Disease,2003,26(9):513-515.
[7]Chen GH,Wang SW,Zhang WL,et al.Clinical analysis of the relationship between sleep apnea-hypopnea syndrome and the morbidity of cardiovascular diseases[J].Chinese Journal of Multiple Organ Diseases in the Elderly,2005,4(4):266-269.
[8]Zhang X,editor in chief.Modern Sleep Medicine[M].Beijing:People's Medical Publishing House,2007:305-311.
[9]WU Q;LI LG;GENG ZH.Changes of cardiomyocyte apoptosis and expression of apoptosis-associated gene during heart failure following myocardial infarction in rats[J].Acta Academiae Medicinae Militaris,2002,24(4):434-436.
[10]TONG H,ZHANG M.Effects of Fasudil at Different Doses on Cardiomyocyte Apoptosis and Protein Expression Levels of bcl-2,c-myc in the Rats with Pressure Overloaded Heart Failure[J].Acta Laboratorium Animalis Scientia Sinica,2007,15(5):376-379.
[11]Solaro RJ.Myosin light chain phosphatase:a Cinderella of cellular signaling[J].J Circ Res,2000,87:173-175.
[12]Shimokawa H.Rho2kinase as a novel therapeutic target in treatment of cardiovascular diseases[J].Cardiovasc Pharma,2002,39:319-327.
[13]Shuaike M,Steven W,J.Thomas C,et al.Chronic intermittent hypoxia sensitizes acute hypothalamic-pituitary-adrenal stress reactivity and Fos induction in the rat locus coeruleus in response to subsequent immobilization stress[J].