中国野生蓝莓花青素对不同食源性致病菌的抑菌机理研究
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- 英文论文题名：Antibacterialmechanism of chinese wild blueberry anthocyanins on various foodborne pathogens
- 论文作者：张玉洁 ; 魏彩虹 ; 孙晓红
- 英文论文作者：Zhang Yujie ; Wei Caihong ; Sun Xiaohong ; College of Food Science and Technology ; Shanghai Ocean University
- 年：2016
- 作者机构：上海海洋大学食品学院;上海水产品加工及贮藏工程技术研究中心;农业部水产品贮藏保鲜质量安全风险评估实验室(上海);
- 论文关键词：中国野生蓝莓 ; 食源性致病菌 ; 抑菌机理 ; 花青素
- 英文论文关键词：chinese wild blueberry ; foodborne pathogens ; antibacterial mechanism ; anthocyanins
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.4
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：120k
- 原文格式：O
- 会议级别：全国
摘要
蓝莓中富含花青素等多酚类物质。花青素的生物活性显示了其在预防慢性退化疾病方面的潜在可能。为了了解花青素如何抑制食源性致病菌的生长,本文研究了中国野生蓝莓对单增李斯特菌,金黄色葡萄球菌、肠炎沙门氏菌、副溶血性弧菌四种食源性致病菌的抑菌作用。结果显示花青素对单增李斯特菌,金黄色葡萄球菌、肠炎沙门氏菌、副溶血性弧菌的最小抑菌浓度分别为0.53、0.27、0.53、0.13 mg/mL。扫描电镜发现花青素能够破坏菌体的原有形态,致使胞内物质泄漏。当花青素作用细菌两小时后,细菌总蛋白含量减少,AKP酶、ATP酶活性分别减少54.3%,69.7%。随着花青素浓度的不断增加,OD630明显降低,细菌代谢能力减弱,继而影响了细菌的能量转换,抑制了其生长及再生。花青素通过破坏细胞膜及其生长环境,影响细菌的新陈代谢,降低了细菌活性。本研究表明中国野生蓝莓花青素具有较强的抑菌活性,可以在预防医学、食品安全等领域加以应用,并丰富了人们对于野生蓝莓有利于健康的认识。
Blueberries are rich in polyphenols such as anthocyanins.Anthocyanins are nutraceuticals which show the potential to prevent chronic degenerative diseases through their biological activities.The objective of this study was to evaluate the anthocyanins how to inhibit the growth of foodborne pathogens.The antibacterial effects of Chinese wild blueberry anthocyanins on 4pathogenic bacteria,L.monocytogenes,S.aureus,S.enteritidls and V.parahaemolyticus,had investigated.The minimum bactericidal concentrations(MBCs) of anthocyanins against L.monocytogenes,S.aureus,S.enteritidis,V.parahaemolyticusrespectively were 0.53,0.27,0.53,0.13 mg/mL.The scanning electron microscopy micrographs shows that anthocyaninsdestroyed the original shape of the cell,so that intracellular material leak.When the bacterias treated with anthocyanins for2 hours,total protein content decreased in bacteriaand the enzyme of AKP,ATP decreased 54.3%,69.7%respectively。With increasing concentration of anthocyanins,the OD_(630) distinctly decrease which shows that bacterial metabolism was decreased,affecting the energy transfer of bacteria and inhibiting their growth and reproduction.Anthocyaninsaffected bacterial metabolismand reduced the bacterial cell activitythrough destroy the cell membrane and the growth environment of bacteria.The Chinese wild blueberry anthocyanins which had strong antibacterial as shown in this study could be applied to the field of preventive medicine,food safety,and enrich the understanding of the health benefits of wild blueberry.
Blueberries are rich in polyphenols such as anthocyanins.Anthocyanins are nutraceuticals which show the potential to prevent chronic degenerative diseases through their biological activities.The objective of this study was to evaluate the anthocyanins how to inhibit the growth of foodborne pathogens.The antibacterial effects of Chinese wild blueberry anthocyanins on 4pathogenic bacteria,L.monocytogenes,S.aureus,S.enteritidls and V.parahaemolyticus,had investigated.The minimum bactericidal concentrations(MBCs) of anthocyanins against L.monocytogenes,S.aureus,S.enteritidis,V.parahaemolyticusrespectively were 0.53,0.27,0.53,0.13 mg/mL.The scanning electron microscopy micrographs shows that anthocyaninsdestroyed the original shape of the cell,so that intracellular material leak.When the bacterias treated with anthocyanins for2 hours,total protein content decreased in bacteriaand the enzyme of AKP,ATP decreased 54.3%,69.7%respectively。With increasing concentration of anthocyanins,the OD_(630) distinctly decrease which shows that bacterial metabolism was decreased,affecting the energy transfer of bacteria and inhibiting their growth and reproduction.Anthocyaninsaffected bacterial metabolismand reduced the bacterial cell activitythrough destroy the cell membrane and the growth environment of bacteria.The Chinese wild blueberry anthocyanins which had strong antibacterial as shown in this study could be applied to the field of preventive medicine,food safety,and enrich the understanding of the health benefits of wild blueberry.
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