高效液相法同时测定抗肿瘤三棱复方提取液中4种化学成分
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- 论文作者：程杰 ; 黄国栋 ; 冯丽雯 ; 刘史佳
- 年：2016
- 作者机构：无锡市锡山人民医院普外科;南京中医药大学药学院;南京中医药大学附属医院临床药理科;
- 论文关键词：抗肿瘤中药 ; 水提物 ; 醇提物 ; 高效液相色谱仪 ; 含量测定
- 英文论文关键词：Anti-tumor traditional Chinese medicine ; Water Extract ; Alcohol Extract ; High Performance Liquid Chromatograph ; Content Determination
- 会议召开时间：2016-11-25
- 会议录名称：湖南中医药大学学报2016/专集：国际数字医学会数字中医药分会成立大会暨首届数字中医药学术交流会论文集
- 语种：中文
- 分类号：R284
- 学会代码：HNZY
- 会议名称：国际数字医学会数字中医药分会成立大会暨首届数字中医药学术交流会
- 会议地点：中国广东珠海
- 主办单位：国际数字医学会、Digital Chinese Medicine
- 学会名称：湖南中医药大学期刊杂志社
- 页数：2
- 文件大小：2077k
- 原文格式：D
- 会议级别：全国
摘要
目的建立高效液相方法同时测定抗癌中药复方(三棱、制大黄、川芎)中4种化学成分,对其水提物和醇提物中各主要成分的含量进行对比,为优化复方提取工艺提供初步依据。方法色谱柱为Zorbax SB-C18(4.6×12.5mm,5μm),流动相为甲醇-0.1%磷酸水,梯度洗脱,分析时间18 min。利用所建立的方法对不同提取批次的水提物(3批)和醇提物(3批)进行含量测定。结果 4种化学成分的峰面积和浓度在测定浓度范围内均具有良好的线性关系,精密度RSD为3.6~8.5%,平均加样回收率为91.2~103.6%。结论本测定方法简便、灵敏度高、专属性好,适用于复方提取液的含量测定。
Objective: To create HPLC approach and determination of Common Burreed Rhizome compound(Common Burreed Rhizome, Prepared Rhubard, Chuanxiong Rhizoma) extractof 4 chemical composition,the water extract and alcohol extract content of each ingredient in the comparison, provide a preliminary basis for optimization of complex extraction process. Method: Chromatographic column for the Zorbax SB- C18(4.6 x 12.5 mm, 5 microns), mobile phase of methanol to 0.1% phosphoric acid water, gradient elution, 18 min analysis time. Using the approach ofdifferent batch of aqueous extract(3 batchs) and alcohol extract content determination(3 batchs). Result: 4 kinds of chemical composition of the peak area and concentration in the determination ofconcentration range has a good linear relationship, precision RSD was 3.6 ~ 8.5%, the recovery is 91.2 ~ 103.6% on average. Conclusion:This determination approach is simple, high sensitivity, good specificity, and is suitable for content determination of the compound extract.
Objective: To create HPLC approach and determination of Common Burreed Rhizome compound(Common Burreed Rhizome, Prepared Rhubard, Chuanxiong Rhizoma) extractof 4 chemical composition,the water extract and alcohol extract content of each ingredient in the comparison, provide a preliminary basis for optimization of complex extraction process. Method: Chromatographic column for the Zorbax SB- C18(4.6 x 12.5 mm, 5 microns), mobile phase of methanol to 0.1% phosphoric acid water, gradient elution, 18 min analysis time. Using the approach ofdifferent batch of aqueous extract(3 batchs) and alcohol extract content determination(3 batchs). Result: 4 kinds of chemical composition of the peak area and concentration in the determination ofconcentration range has a good linear relationship, precision RSD was 3.6 ~ 8.5%, the recovery is 91.2 ~ 103.6% on average. Conclusion:This determination approach is simple, high sensitivity, good specificity, and is suitable for content determination of the compound extract.
引文
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[10]梁惠珍,李先荣,王瑞民.薄层扫描法测定复方脑清胶囊中盐酸水苏碱的含量[J].中国药事,2009,23(6):575-576.
[11]李莉,宋俊骊,王志梅,等.高效液相色谱法同时测定九制大黄丸中5组分含量[J].中国药业,2015,24(5):34-35.
[2]沈红梅,程涛,乔传卓等.乌梅的体外抗肿瘤活性及免疫调节作用初探[J].中国中药杂志,1995,20(6):365-384.
[3]高杰,邓述恺.苦参抗肿瘤作用机制的研究进展[J].中国医院用药评价与分析,2010,10(12):1151-1152.
[4]孙健,温庆辉,宋宇等.黄连解毒汤抗肿瘤作用的实验研究[J].中国中药杂志,2006,31(17):1461-1463.
[5]丁艳,黄志华.大黄素的抗肿瘤作用研究进展[J].时珍国医国药,2008,19(5):1272-12774.
[6]吴昊,关树光,於文博等.中药三棱的化学成分指认和指纹图谱研究[J].中国医药指南,2012,10(34):431-433.
[7]周婧,吴启南,戴仕林等.综合主成分分析用于中药三棱质量评价[J].中医药学报,2012,40(6):25-28.
[8]龚学忠,王跃荣,符丹等.制大黄-川芎药对对造影剂肾病大鼠肾小管上皮细胞凋亡的影响[J].中国中西医结合肾病杂志,2012,13(8):675-677.
[9]张海波,高羽,梁侨丽,等.HPLC测定中药三棱中游离阿魏酸和总阿魏酸含量[J].南京中医药大学学报,2011,27(2):169-171.
[10]梁惠珍,李先荣,王瑞民.薄层扫描法测定复方脑清胶囊中盐酸水苏碱的含量[J].中国药事,2009,23(6):575-576.
[11]李莉,宋俊骊,王志梅,等.高效液相色谱法同时测定九制大黄丸中5组分含量[J].中国药业,2015,24(5):34-35.