抗菌抗病毒杂合肽C-L的抑菌谱及在模拟胃肠液中稳定性的研究
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- 英文论文题名：Study on antimicrobial spectrum and digestive stability in simulative digestive tract fluid of antibacterial and antiviral hybridpeptide C-L
- 论文作者：张璐璐 ; 卫旭彪 ; 商婷婷 ; 张日俊
- 英文论文作者：ZHANG Lulu ; WEI Xubiao ; SHANG Tingting ; ZHANg Rijun ; College of Animal Science and Technology ; China Agricultural University
- 年：2016
- 作者机构：中国农业大学动物科技学院;
- 论文关键词：饲料微生物学 ; 抗菌抗病毒杂合肽C-L ; 抑菌谱 ; 抗菌性能 ; 模拟胃肠液 ; 稳定性
- 英文论文关键词：feed microbiology ; antibacterial and antiviral hybridpeptide ; antimicrobial spectrum ; antimicrobial properties ; simulated gastrointestinal fluid ; stability
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：S816
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：96k
- 原文格式：O
- 会议级别：全国
摘要
为分析实验室前期筛选获得的高效抗菌抗病毒杂合肽CecropinA(1-8)-LL37(17-30)(简称C-L)的抑菌谱及在模拟胃肠液中的稳定性,首先利用牛津杯琼脂扩散法,检测其对9种指示菌的抑制效果;然后通过模拟胃肠液处理,观察其抑菌活性变化。结果表明:抗菌抗病毒杂合肽C-L对多种致病菌(金黄色葡萄球菌ATCC43300、金黄色葡萄球菌CVCC1882、金黄色葡萄球菌耐药菌100621、李斯特菌CVCC1599、沙门氏菌CVCC2212、大肠杆菌245)均具有良好的抑制效果,并对益生地衣芽孢杆菌CGMCC1.807无明显的抑菌作用,且抗菌肽C-L经过模拟胃肠液处理后依然保持约90%的抑菌活性。由此表明,该抗菌肽具有良好的抑菌特性及抵抗模拟胃肠液消化的稳定性,对设计优良特性的抗菌肽具有重要的启示,并在健康养殖中具有广阔的应用潜力和价值。
In order to analyze antimicrobial spectrum and digestive stability in simulative digestive tract fluid of antibacterial and antiviral hybridpeptide C-L obtained from the previous research in our lab,the inhibitory effect on nine kinds of indicator bacteria was detected by the Oxford cup method and the simulative digestive experiment in vitro was carried out.The experimental results indicate that C-L has good antibacterial effect on several bacteria(Staphylococcus aureus ATCC43300,S.aureus CVCC1882,Multiple-resistant S.aureus 100621,Lester CVCC1599,Salmonella CVCC2212,Escherichia coli 245),but does not show obvious antibacterial activity on beneficial bacteria Baclicus lincheniformis CGMCC 1.807,and remains about 90%antibacterial activity after the treatment of digestion.In conclusion,antibacterial and antiviral hybridpeptide C-L has good antimicrobial characteristics and stability in the gastrointestinal fliud treatment,which shows great application potential in livestock husbandary production.
In order to analyze antimicrobial spectrum and digestive stability in simulative digestive tract fluid of antibacterial and antiviral hybridpeptide C-L obtained from the previous research in our lab,the inhibitory effect on nine kinds of indicator bacteria was detected by the Oxford cup method and the simulative digestive experiment in vitro was carried out.The experimental results indicate that C-L has good antibacterial effect on several bacteria(Staphylococcus aureus ATCC43300,S.aureus CVCC1882,Multiple-resistant S.aureus 100621,Lester CVCC1599,Salmonella CVCC2212,Escherichia coli 245),but does not show obvious antibacterial activity on beneficial bacteria Baclicus lincheniformis CGMCC 1.807,and remains about 90%antibacterial activity after the treatment of digestion.In conclusion,antibacterial and antiviral hybridpeptide C-L has good antimicrobial characteristics and stability in the gastrointestinal fliud treatment,which shows great application potential in livestock husbandary production.
引文
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[2]SETHI P M,MIRANDA J J,KADIYALA S,et al.Evaluation of autologous platelet concentrate for intertransverse process lumbar fusion[J].American journal of orthopedics(Belle Mead,N.J.),2008,37(4):E84-E90.
[3]SCHMIDMAIER G,HERRMANN S,GREEN J,et al.Quantitative assessment of growth factors in reaming aspirate,iliac crest,and platelet preparation[J].Bone,2006,39(5):1156-1163.
[4]ANDREU D,UBACH J,BOMAN A,et al.Shortened cecropin-a melittin hybrids-significant size-reduction retains potent antibiotic-activity[J].Febs Letters,1992,296(2):190-194.
[5]王秀青,朱明星,张爱君,等.天蚕素类杂合肽cecropinA-magainin突变体的合成以及在毕赤酵母中的分泌表达[J].东北农业大学学报,2009(11):95-98.
[6]CAO Y,YU R Q,LIU Y,et al.Design,Recombinant expression,and antibacterial activity of the Cecropins-Melittin hybrid antimicrobial peptides[J].Current Microbiology,2010,61(3):169-175.
[7]王庆.高效杂合抗菌抗病毒肽分子设计及外源表达研究[D].北京:中国农业大学,2013.
[8]武如娟.抗菌抗病毒杂合肽Cecropin A(1-8)-LL37(17-30)抗菌机理、重组表达和特性研究[D].北京:中国农业大学,2015.
[9]TURGIS M,KHANH D V,LACROIX M.Partial characterization of bacteriocins produced by two new Enterococcus faecium isolated from human intestine[J].Probiotics and Antimicrobial Proteins,2013,5(2):110-120.
[10]冼小敏,侯雁,林洁茹,等.燕窝蛋白质在模拟胃肠环境中的降解研究[J].中药材,2010(11):1760-1763.