智能响应型微胶囊在细胞内的控制释放研究
详细信息 查看官网全文
- 英文论文题名：The Research of Intelligent Response Microcapsules with Programmable Active Release
- 论文作者：刘建军 ; 王秉 ; 苏孝鹏 ; 胡智文
- 英文论文作者：Jianjun Liu ; Bing Wang ; Xiaopeng Su ; Zhiwen Hu ; College of Materials and Textiles ; Zhejiang Sci-Tec University
- 年：2016
- 作者机构：浙江理工大学材料与纺织学院;
- 论文关键词：微胶囊 ; 包埋 ; 自组装 ; 智能响应
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十八分会：化学生物学
- 语种：中文
- 分类号：TQ460.1
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十八分会 ; 化学生物学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：145k
- 原文格式：D
- 会议级别：全国
摘要
目前抗乙肝病毒的药物干扰素存在半衰期短,肾脏清除率高以及系统分布广等缺点,容易产生抗药性和其他毒副作用。本文以MnCO_3微粒为模板,3,3′-二硫代二丙酸(DPA)为交联剂,采用层层组装法制备了共价交联的(PAH/DPA)_5微胶囊,将药物干扰素包埋在其中,该方法兼具模板法与层层自组装法制备微囊的优势,所制备的微胶囊具有成分可选择、尺寸可控、囊壁厚度可调节以及易通过功能组分的引入赋予胶囊智能响应性等优点。经过TEM,SEM,AFM,CLSM等表征发现该微胶囊粒径在4-6μm左右,壁厚约为55.25±1.62nm,药物包埋率约为45.24%,且能够在细胞外部保持稳定形态,在细胞内部时由于谷胱甘肽以及pH值的变化,导致微胶囊的降解,从而释放出包埋的干扰素药物,达到智能响应释放药物的效果。在与Hep-G2细胞的共培养中发现微囊对细胞活性并没有明显影响。
In this study,MnCO_3 particles were used as templates cores and DPA as the crosslinking agent for preparing the(PAH/DPA)_5 microcapsules of covalent crosslinking using layer-by-layer assembly method,and the interferon drugs were embedded in microcapsules.By taking the advantage of both templates and layer-by-layer assembly,microcapsules that have selectable composition,controllable size and wall thickness were prepared successfully,and have intelligent response by introducing functional component.Results of the TEM,SEM,AFM and CLSM characterization analysis showed that the particle size of microcapsules were determined at about 4-6 μm,the wall thickness were 55.25 ± 1.62 nm and had 45.24% embedding rate.This kind of microcapsules were stable outside the cell,but degraded because of the change of glutathione and pH inside the cell,the interferon drugs were released thereby and reached intelligent response.No significant effect on cell viability was noticed when the microcapsules were in co-cultured with Hep-G2 cells.
In this study,MnCO_3 particles were used as templates cores and DPA as the crosslinking agent for preparing the(PAH/DPA)_5 microcapsules of covalent crosslinking using layer-by-layer assembly method,and the interferon drugs were embedded in microcapsules.By taking the advantage of both templates and layer-by-layer assembly,microcapsules that have selectable composition,controllable size and wall thickness were prepared successfully,and have intelligent response by introducing functional component.Results of the TEM,SEM,AFM and CLSM characterization analysis showed that the particle size of microcapsules were determined at about 4-6 μm,the wall thickness were 55.25 ± 1.62 nm and had 45.24% embedding rate.This kind of microcapsules were stable outside the cell,but degraded because of the change of glutathione and pH inside the cell,the interferon drugs were released thereby and reached intelligent response.No significant effect on cell viability was noticed when the microcapsules were in co-cultured with Hep-G2 cells.
引文
[1]Wang B,Zhang Y,Mao Z,et al.Cellular uptake of covalent poly(allylamine hydrochloride)microcapsules and its influences on cell functions[J].Macromolecular bioscience,2012,12(11):1534-1545.
[2]Liu W,Zhou X,Mao Z,et al.Uptake of hydrogel particles with different stiffness and its influence on HepG2cell functions[J].Soft Matter,2012,8(35):9235-9245.
[2]Liu W,Zhou X,Mao Z,et al.Uptake of hydrogel particles with different stiffness and its influence on HepG2cell functions[J].Soft Matter,2012,8(35):9235-9245.