甘草次酸脂质体的制备与性质研究
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- 英文论文题名：Preparation and Characterization of Glycyrrhetinic-acid loaded liposome
- 论文作者：贾红娇 ; 张婉萍
- 英文论文作者：Hong-jiao Jia ; Wan-ping Zhang ; Shanghai Institute of technology
- 年：2016
- 作者机构：上海应用技术学院香料香精技术与工程学院;
- 论文关键词：甘草次酸 ; 脂质体 ; 制备 ; 表征
- 英文论文关键词：Gycyrrhetinic acid ; Liposome ; Preparation ; Characterization
- 会议召开时间：2016-06-15
- 会议录名称：第十一届中国化妆品学术研讨会论文集
- 英文会议录名称：The Proceedings of the 11th Seminar on Cosmetic China
- 语种：中文
- 分类号：TQ658
- 学会代码：ZGXE
- 会议名称：第十一届中国化妆品学术研讨会
- 会议地点：中国上海
- 主办单位：中国香料香精化妆品工业协会
- 学会名称：中国香料香精化妆品工业协会
- 页数：7
- 文件大小：688k
- 原文格式：O
- 会议级别：全国
摘要
目的:优化甘草次酸(GA)脂质体的工艺配方并考察其体外应用性能。方法:采用溶剂-超声法制备GA脂质体,以粒径、稳定性为综合评价指标,优化制备工艺及配方,扫描电镜观察脂质体的粒径形态及大小。利用高效液相色谱法测定GA含量,进行体外释放和透皮实验的表征。结果:GA脂质体最佳工艺处方为超声时间2min;超声功率30%;吐温-80加入量0.33%,氯化钠加入量为0.05%。制备的脂质体粒径均匀,呈球形小囊泡。分散性好,粒径(117±4.64)nm,包封率(91.0±1.21)%;GA脂质体具有明显的缓释效果,可有效作用于真皮层中;GA脂质体在4℃的存放条件下,30d内粒径变化较小,稳定性良好。
Purpose:Optimization the formula of Glycyrrhetinic acid(GA) liposome and investigate its application performance in vitro.Mehtods:Solvent-ultrasonic method was used to prepare GA liposome;Using particle size and stability as the comprehensive evaluation index to optimize the preparation technology and formula;Scanning Electron Microscope was used to observe the particle morphology and size of liposomes.The GA content was determined by High Performance Liquid Chromatography(HPLC) method for the characterization of the in vitro release and transdermal experiments.Results:The optimum process recipe for GA liposome was as follows:the ultrasonic time was 2 min;the ultrasonic power was 30%;the Tween-80 content was 0.33%and the amount of sodium chloride was 0.05%.The prepared liposome was of uniform particle size and present spherical capsule.The particle size was(117±4.64) nm,the encapsulation efficiency was(91.0± 1.21)%.GA liposome had obvious sustained release effect and can be effectively act in the dermis.Under the storage condition of 4 ℃,the particle size GA liposome was rarely changed within 30 days which demonstrated he stability was good.
Purpose:Optimization the formula of Glycyrrhetinic acid(GA) liposome and investigate its application performance in vitro.Mehtods:Solvent-ultrasonic method was used to prepare GA liposome;Using particle size and stability as the comprehensive evaluation index to optimize the preparation technology and formula;Scanning Electron Microscope was used to observe the particle morphology and size of liposomes.The GA content was determined by High Performance Liquid Chromatography(HPLC) method for the characterization of the in vitro release and transdermal experiments.Results:The optimum process recipe for GA liposome was as follows:the ultrasonic time was 2 min;the ultrasonic power was 30%;the Tween-80 content was 0.33%and the amount of sodium chloride was 0.05%.The prepared liposome was of uniform particle size and present spherical capsule.The particle size was(117±4.64) nm,the encapsulation efficiency was(91.0± 1.21)%.GA liposome had obvious sustained release effect and can be effectively act in the dermis.Under the storage condition of 4 ℃,the particle size GA liposome was rarely changed within 30 days which demonstrated he stability was good.
引文
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[2].程怡,郭波红,林绿萍.甘草次酸脂质体的包封率测定和体外释放度考察[J].广州中医药大学学报2010,27(4):384~388.
[3].表面活性剂对茶多酚脂质体的制备及透皮性能的影响[J].东南大学学报,37(1):131~135.
[4].旷英姿,马全红,郝晓帧,等.茶多酚脂质体的制各研究[J].中国生化药物杂志,2006,27(1):28~31.
[5].刘硕,仵文英,席枝侠,等.黄芩苷脂质体的包封率测定和体外释放度考察[J].中国医院药学杂志,2008,28(5):342.
[6].刘迎春,宋学君,王国清.RP-HPLC法测定玄麦甘桔颗粒中GA含量[J].沈阳药科大学学报,2006,23(6):376~406.
[7].顾宜,石玉,张三奇,等.大黄素纳米脂质体的制备和质量评价[J].第四军大学学报,2003,24(5):479.
[8].郭芳,胡国胜,奚朝晖,等.化妆品用原花青素脂质体的制备及应用性能研究[J].日用化学工业,2014,44(3):143~150.
[9].刘高梅,任海伟.葵花籽油脂质体的制备及其稳定生研究[J].中国粮油学报,2011,26(11):47~51.
[10].廖建华,刘玉珍,熊华.薏苡仁油脂质体特性及体外释放度的研究[J].中国油脂,2011,36(11):36~41.
[11].丁丽燕,杨春,李学明,等.乙醇注入法制备司帕沙星脂质体[J].南京工业大学学报,2007,29(1):32~35.
[12].李惠敏,郑明彬,王柯敏,等.包裹姜根油的多种纳米颗粒的制备及质量评价[J].湖南大学学报,2006,33(3):93~97.
[13].杨志文,杨木华,夏侯国论,等.莪术油前体脂质体的制备及其质量评价[J].中国药学杂志,2008,43(19):1488.
[14].姚世霞,倪京满,钱松,等.当归挥发油脂质体工艺研究[J].中成药,2007,29(1):54~57.
[15].时军,程怡,陈伟鸿,等.足叶长循环脂质体的制备及血浆中稳定性研究[J].广州中医药大学学报,2009,26(3):270~273.
[16].陈建明,张仰眉,高中.维生素A前体脂质体的研制及其特性考察[J].第二军医大学学抿2003,24(2):207~209.
[17].陈智娴.中药脂质体研究进展[J].实用中医药杂志,2010,26(8):587~589.
[18].周蔚,周彩存,孟淑燕,等.靶向肺癌的紫杉醇长循环脂质体抑瘤作用研究[J].肿瘤,2011,31(3):203~209.
[19].陈柳华,甘礼华,赵云辉,等.氟比洛芬脂质体的制备及其载药性能研究[J].同济大学学报,2011,39(7):1079~1083.