一种高灵敏的氨基硫脲罗丹明B汞离子荧光探针的合成及应用
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- 英文论文题名：The synthesis and application of a highly sensitive thiosemicarbazide rhodamine B fluorescent probe for mercury ion
- 论文作者：时蕾 ; 鹿泽华 ; 张伶俐 ; 董燕平 ; 张贵生
- 英文论文作者：Lei Shi ; Zehua Lu ; Lingli Zhang ; Yanping Dong ; Guisheng Zhang ; School of Chemistry and Chemical Engineering ; Henan Normal University
- 年：2016
- 作者机构：河南师范大学化学化工学院;
- 论文关键词：罗丹明B ; 氨基硫脲荧光探针 ; 汞离子识别
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十七分会：光化学
- 语种：中文
- 分类号：O657.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十七分会 ; 光化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：2
- 文件大小：378k
- 原文格式：D
- 会议级别：全国
摘要
Hg~(2+)离子是一种生理毒性非常强的重金属离子,容易通过食物链进行生物富集从而对人类的内分泌系统和心脑血管产生很强的破坏能力,不易被代谢出体外1。由于Hg~(2+)离子广泛存在于海水和土壤中,近年来研究如何对Hg~(2+)离子进行快速灵敏地检测成为环境分析和生命科学领域研究的热点之一。化学家合成了多种不同类型的汞离子荧光探针并研究了汞离子的识别机理,但仍然存在探针分子合成步骤复杂,在水相中溶解性较差等缺点2。罗丹明类氨基硫脲荧光探针在水相或细胞内都对汞离子有高灵敏度及专一识别的特点3。我们以罗丹明B为原料合成了邻氟苯基罗丹明氨基硫脲荧光探针(RFS),系统研究了探针对汞离子的识别作用及机理,结果表明探针具有灵敏度高,对汞离子选择性强,能够实现纯水溶剂中识别等优点。
Mercury is one of the most prevalent toxic metals in the environment, easily passes through biol ogical membranes such as skin, respiratory and gastrointestinal tissues. As the widespread exist in ocean and soil,the research of how to rapidly and sensitively detect the Hg~(2+) become one of the hot focuses in environmental analysis and life sciences. A variety of fluorescent probes for Hg~(2+) have been synthesized and studied, but there are also some problems such as complex synthetic steps, poor solubility in water and so on. Rhodamine B thiosemicarbazide-based fluorescent probe for Mercury ion both have the charact eristics of high sensitivity and specificity in water. We synthesized the thiosemicarbazide rhodamine B-base d fluorescent probe by rhodamine B and isothiocyanate. We systematically studied the probe's recognition for Hg~(2+) and its mechanism.Our results show that the probe has high sensitivity and selectively recognize for mercury ion in water and in living Hela cells.
Mercury is one of the most prevalent toxic metals in the environment, easily passes through biol ogical membranes such as skin, respiratory and gastrointestinal tissues. As the widespread exist in ocean and soil,the research of how to rapidly and sensitively detect the Hg~(2+) become one of the hot focuses in environmental analysis and life sciences. A variety of fluorescent probes for Hg~(2+) have been synthesized and studied, but there are also some problems such as complex synthetic steps, poor solubility in water and so on. Rhodamine B thiosemicarbazide-based fluorescent probe for Mercury ion both have the charact eristics of high sensitivity and specificity in water. We synthesized the thiosemicarbazide rhodamine B-base d fluorescent probe by rhodamine B and isothiocyanate. We systematically studied the probe's recognition for Hg~(2+) and its mechanism.Our results show that the probe has high sensitivity and selectively recognize for mercury ion in water and in living Hela cells.
引文
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3 a.Sung-Kyun Ko,Young-Keun Yang,Jinsung Tae,and Injae Shin.In Vivo Monitoring of Mercury Ions Using a Rhodamine-Based Molecular Probe[J].J.AM.CHEM.SOC.2006,128(43),14150-14155.b.Chunhua Ma,Liping Lin,Yanyan Du,Liang-bi Chen,Feng Luo and Xi Chen.Fluorescence quenching determination of iron(III)using rhodamine 6G hydrazide derivative[J].Anal.Method,2013,5,:1843-1847.c.Young-Keun Yang,Sung-Kyun et al.A chromogenic macrocycle exhibitingcation-selective andanion-controlled color change:an approach tounderstanding structure-color relationships[J].Org.Biomol.Chem.,2009(7),4590-4593.