表面活性剂聚集体型荧光传感器对Asp和Glu的选择性检测
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- 英文论文题名：Surfactant Assemblies-based Fluorescent Sensor for Selective Detection of Asp and Glu in Aqueous Solution
- 论文作者：张园园 ; 曹建华 ; 丁立平
- 英文论文作者：Yuanyuan Zhang ; Jianhua Cao ; Liping Ding ; School of Chemistry and Chemical Engineering ; Shaanxi Normal University
- 年：2016
- 作者机构：陕西师范大学化学化工学院应用表面与胶体化学教育部重点实验室;
- 论文关键词：荧光传感器 ; 氨基酸 ; 超分子组装
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十一分会：胶体与界面化学
- 语种：中文
- 分类号：TP212;O657.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十一分会 ; 胶体与界面化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：293k
- 原文格式：D
- 会议级别：全国
摘要
氨基酸是构成蛋白质的基本单元,在人的生命过程中起着重要的作用。其中天冬氨酸(Asp)和谷氨酸(Glu)属于兴奋性氨基酸,对于哺乳动物的大脑功能有着至关重要的影响~([1])。表面活性剂由于其两亲性结构可以在水溶液中形成非均相的超分子组装体,如胶束、囊泡等,可以为荧光探针提供疏水微区。本工作中,利用阳离子型丹磺酰衍生物(DI1SD)和带负电荷的表面活性剂(SDS)聚集体构建传感平台,实现了在水溶液中对Asp和Glu的选择性识别。该传感体系的荧光发射随着天冬氨酸浓度的增大而显著减弱(Fig.1a)。紫外光谱测定研究表明,Asp的引入会产生新的吸收带(Fig.1b),表明Asp与传感探针发生了结合。酸碱实验、核磁滴定实验等结果表明天Asp和Glu分离出的H~+与丹磺酰分子中叔氨基的结合,是导致光谱变化的主要原因,而SDS聚集体的存在是实现传感的必要条件。
Amino acids are the basic units of biological proteins and play an important role in the life processes.Amphiphilic surfactant molecules can form heterogeneous supramolecular assemblies in aqueous solution and provide hydrophobic microdomains for lipophilic fluorophores.In this work,we used a cationic dansyl-based derivative(DIISD) and anionic surfactant(SDS) to build a supramolecular sensor platform for selective sensing Asp/Glu in aqueous solution.Fluorescence studies reveal that the fluorescent sensor exhibits reduced fluorescence upon titration of Asp and Glu.UV-vis absorption measurements find that new absorption band appears along the addition of Asp or Glu,suggesting the binding of these two amino acids with the fluorophore.~1H NMR titration reveals that the binding of H~+ from Asp and Glu with the ternary amine group in DIISD causes the fluorescence quenching.It is also found that SDS assemblies play an important role in the sensing of Asp and Glu.
Amino acids are the basic units of biological proteins and play an important role in the life processes.Amphiphilic surfactant molecules can form heterogeneous supramolecular assemblies in aqueous solution and provide hydrophobic microdomains for lipophilic fluorophores.In this work,we used a cationic dansyl-based derivative(DIISD) and anionic surfactant(SDS) to build a supramolecular sensor platform for selective sensing Asp/Glu in aqueous solution.Fluorescence studies reveal that the fluorescent sensor exhibits reduced fluorescence upon titration of Asp and Glu.UV-vis absorption measurements find that new absorption band appears along the addition of Asp or Glu,suggesting the binding of these two amino acids with the fluorophore.~1H NMR titration reveals that the binding of H~+ from Asp and Glu with the ternary amine group in DIISD causes the fluorescence quenching.It is also found that SDS assemblies play an important role in the sensing of Asp and Glu.
引文
[1]Zhao,X.;Wei,R.;Chen,L.;Yan,X.New J.Chem.,2014,38,4791-4798.