槜李果实采后生理特性及乙烯生物合成相关酶活性的研究
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摘要
本研究以榜李果实为材料,分别进行外源乙烯、减压浸钙和低温贮藏处理,研究各处理条件下携李果实成熟软化过程中硬度、乙烯释放量、ACS和ACO活性等指标的变化。结果表明:携李果实软化过程中,硬度逐渐降低,乙烯释放量和ACS活性的峰值同时出现在软化后期;外源乙烯(0.5μl·L~(-1))处理后,果实硬度迅速下降,乙烯跃变提前,乙烯释放量增大,ACS活性增强,促进果实迅速软化;用CaCl_2溶液(2%)浸泡处理显著减缓了果实软化,降低了乙烯释放速率;低温贮藏显著抑制了果实硬度下降,表明一定条件的低温贮藏对乙烯释放速率及其相关酶活的控制效果极为显著。
引文
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[6]Khan A S,Singh Z.1-MCP regulates ethylene biosynthesis and fruit softening during ripening of'Tegan Blue'plum.Postharvest Biology and Technology,2007,43(3):298-306.
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[8]Oeller P W,Lu M W,Taylor L P,et al.Reversible inhibition of tomato fruit senescence by antisense RNA.Science,1991,254(5030):437-439.
[9]Tatsuki M,Haji T,Yamaguchi M.The involvement of 1-aminocyclopropane-l-carboxylic acid synthase isogene,Pp-ACSl,in peach fruit softening[J].Journal of Experimental Botany,2006,57(6):1281-1289.
[10]肖红梅,王薛修.钙处理对桃采后生理和贮藏品质的影响[J].南京农业大学学报.1993,19(3):122-129.
[11]吴友根,陈金印,左俊锋.钙渗入抑制翠冠梨果实衰老软化作用的生理生化机制[J].西北植物学报,2003,23(10):1682-1687.
[12]阚娟,金昌海,汪志君,等.β-半乳糖苷酶及多聚半乳糖醛酸酶对桃果实成熟软化的影响[J].扬州大学学报(农业与生命科学版),2006,27(3):76~80.
[13]Ponce N M A,Ziegler V H,Stortz C A,et al.Compositional changes in cell wall polysaccharides from Japanese plum(Prunus salicina Lindl.)during growth and on-tree ripening[J].Journal of Agricultural and Food Chenistry.2010,58:2562-2570.
[14]Wu F W,Zhang D D,Zhang H Y,et al.Physiological and biochemical response of harvested plum fruit to oxalicacid during ripening or shelf-life[J].Food Research International.2011,44(5):1299-1305.
[2]Leshem YY,Pinchasov Y.Non-invasive photoacoustic spectroscopic determination of relative endogenous nitric oxide and ethylene content stoichiometry during the ripening of strawberries Fragaria anannasa(Duch.)and avocados Persea americana(Mill.).J Exp Bot.2000,51(349):1471-3.
[3]Gutierrez M.S.,Trinchero GD.,Cerri A.M.,et al.Different responses of goldenberry fruit treated at four maturity stages with the ethylene antagonist 1-methylcyclopropene.Postharvest Biology and Technology,2008,48(2):199-205.
[4]Xu X B,Tian S P.Salicylic acid alleviated pathogen-induced oxidative stress in harvested sweet cherry fruit.Postharvest Biology and Technology,2008,49(3):379-385.
[5]Rzewuski G,Sauter M.Ethylene biosynthesis and signaling in rice.Plant Science,2008,175(1-2):32-42.
[6]Khan A S,Singh Z.1-MCP regulates ethylene biosynthesis and fruit softening during ripening of'Tegan Blue'plum.Postharvest Biology and Technology,2007,43(3):298-306.
[7]齐玉洁,贾惠娟等.2个榜李优系成熟过程中果实品质及生物活性物质的变化[J].浙江大学学报(农业与生命科学版),2014,40(5):505~510.
[8]Oeller P W,Lu M W,Taylor L P,et al.Reversible inhibition of tomato fruit senescence by antisense RNA.Science,1991,254(5030):437-439.
[9]Tatsuki M,Haji T,Yamaguchi M.The involvement of 1-aminocyclopropane-l-carboxylic acid synthase isogene,Pp-ACSl,in peach fruit softening[J].Journal of Experimental Botany,2006,57(6):1281-1289.
[10]肖红梅,王薛修.钙处理对桃采后生理和贮藏品质的影响[J].南京农业大学学报.1993,19(3):122-129.
[11]吴友根,陈金印,左俊锋.钙渗入抑制翠冠梨果实衰老软化作用的生理生化机制[J].西北植物学报,2003,23(10):1682-1687.
[12]阚娟,金昌海,汪志君,等.β-半乳糖苷酶及多聚半乳糖醛酸酶对桃果实成熟软化的影响[J].扬州大学学报(农业与生命科学版),2006,27(3):76~80.
[13]Ponce N M A,Ziegler V H,Stortz C A,et al.Compositional changes in cell wall polysaccharides from Japanese plum(Prunus salicina Lindl.)during growth and on-tree ripening[J].Journal of Agricultural and Food Chenistry.2010,58:2562-2570.
[14]Wu F W,Zhang D D,Zhang H Y,et al.Physiological and biochemical response of harvested plum fruit to oxalicacid during ripening or shelf-life[J].Food Research International.2011,44(5):1299-1305.