麻黄水煎液及其拆分组分利尿作用的研究
详细信息 查看官网全文
- 英文论文题名：Study on water decoction and diuretic effects of Ephedrae Herba split components
- 论文作者：李苗 ; 曾梦楠 ; 吴广操 ; 韩杜菀 ; 杨胜利 ; 张靖柯 ; 克迎迎 ; 王小兰 ; 冯卫生 ; 郑晓珂
- 年：2016
- 作者机构：河南中医药大学;呼吸疾病诊疗与新药研发河南省协同创新中心;
- 论文关键词：麻黄 ; 利尿作用 ; 机制
- 英文论文关键词：Ephedrae Herba ; Diuresis ; Mechanism
- 会议召开时间：2016-09-27
- 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
- 语种：中文
- 分类号：R285
- 学会代码：YYWS
- 会议名称：中国药学会第十三届青年药学科研成果交流会
- 会议地点：中国江西南昌
- 主办单位：中国药学会
- 学会名称：中国药学会
- 页数：3
- 文件大小：197k
- 原文格式：O
- 会议级别：全国
摘要
目的:研究麻黄的利尿作用,并初步探讨其作用机制。方法:大鼠代谢笼法,观察麻黄水煎液及各拆分组分对大鼠尿量的影响。微板法检测大鼠尿液氯(Cl~-)、钾离子(K~+)水平;比浊法检测大鼠尿液钠(Na~+)水平;ELISA法检测肾素(Renin)、醛固酮(ALD)水平。结果:与空白组相比,麻黄水煎液、生物碱具有利尿效果(P<0.01或P<0.05);麻黄能够降低Renin、ALD水平(P<0.01或P<0.05),增加电解质的排泄。结论:麻黄水煎液具有利尿作用,其机制可能与RAAS系统有关。
Objective:Diuretic effect of Ephedrae Herba,and to discuss its mechanism of action.Methods:rat metabolic cage method,observation of Ephedra Decoction and the separation effect of components on normal rat urine volume.Detection of urinary chloride(Cl~-) and potassium(K~+) levels in rats by micro plate method;sodium(Na~+) levels were detected by turbidity method;ELISA method was used to detect the levels of renin(Renin) and aldosterone(ALD).Results:compared with the blank group,the decoction of ephedra alkaloids has diuretic effect(P<0.01 or P<0.05);ephedra can reduce Renin,ALD level(P<0.01 or P<0.05),increase the excretion of electrolytes.Conclusion:Chinese ephedra decoction has a diuretic effect,its mechanism may be related to RAAS system.
Objective:Diuretic effect of Ephedrae Herba,and to discuss its mechanism of action.Methods:rat metabolic cage method,observation of Ephedra Decoction and the separation effect of components on normal rat urine volume.Detection of urinary chloride(Cl~-) and potassium(K~+) levels in rats by micro plate method;sodium(Na~+) levels were detected by turbidity method;ELISA method was used to detect the levels of renin(Renin) and aldosterone(ALD).Results:compared with the blank group,the decoction of ephedra alkaloids has diuretic effect(P<0.01 or P<0.05);ephedra can reduce Renin,ALD level(P<0.01 or P<0.05),increase the excretion of electrolytes.Conclusion:Chinese ephedra decoction has a diuretic effect,its mechanism may be related to RAAS system.
引文
[1]国家药典委员会.中华人民共和国药典[S].一部.北京:中国医药科技出版社,2010:321.
[2]陆燕萍,刘佳丽,巩晓宇等.麻黄药理作用及含量测定的研究进展[J].中国医药导报,2013,24:38-40.
[3]刘忻.中医经典论治肾系水肿[D].北京中医药大学,2011.
[4]Salyer Sarah A,Parks Jason,Barati Michelle T,Lederer Eleanor D.Clark Barbara J,Klein Janet D,Khundmiri Syed J.Aldosterone regulates Na(+),K(+)ATPase activity in human renal proximal tubule cells through mineralocorticoid receptor.[J].Biochimica et biophysica acta,2013,183310:.
[5]Cao Wengang,Pavlinec Christopher,Gravenstein Nikolaus.Seubert Christoph N,Martynyuk Anatoly E.Roles of aldosterone and oxytocin in abnormalities caused by sevoflurane anesthesia in neonatal rats.[J].Anesthesiology.2012,1174:.
[2]陆燕萍,刘佳丽,巩晓宇等.麻黄药理作用及含量测定的研究进展[J].中国医药导报,2013,24:38-40.
[3]刘忻.中医经典论治肾系水肿[D].北京中医药大学,2011.
[4]Salyer Sarah A,Parks Jason,Barati Michelle T,Lederer Eleanor D.Clark Barbara J,Klein Janet D,Khundmiri Syed J.Aldosterone regulates Na(+),K(+)ATPase activity in human renal proximal tubule cells through mineralocorticoid receptor.[J].Biochimica et biophysica acta,2013,183310:.
[5]Cao Wengang,Pavlinec Christopher,Gravenstein Nikolaus.Seubert Christoph N,Martynyuk Anatoly E.Roles of aldosterone and oxytocin in abnormalities caused by sevoflurane anesthesia in neonatal rats.[J].Anesthesiology.2012,1174:.