线粒体COⅠ基因在厦门海域鱼类鉴定中的适用性分析
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- 英文论文题名：Species identification for fishes in Xiamen sea based on CO Ⅰ barcoding
- 论文作者：邢炳鹏 ; 林汝榕 ; 王彦国 ; 张稚兰
- 英文论文作者：XING Bing-peng ; LIN Ru-rong ; WANG Yan-guo ; ZHANG Zhi-lan ; Third Institute of Oceanography ; SOA
- 年：2016
- 作者机构：国家海洋局第三海洋研究所;
- 论文关键词：海洋生物学 ; 鱼类 ; 细胞色素C氧化酶亚基Ⅰ基因 ; DNA条形码 ; 物种鉴定 ; 厦门海域
- 英文论文关键词：marine biology ; fishes ; CO I ; DNA barcoding ; Species identification ; Xiamen sea
- 会议召开时间：2016-11-11
- 会议录名称：福建省海洋学会2016年学术年会论文摘要集
- 语种：中文
- 分类号：Q953
- 学会代码：FJHY
- 会议名称：福建省海洋学会2016年学术年会
- 会议地点：中国福建厦门
- 主办单位：福建省海洋学会、福建省水产研究所
- 学会名称：福建省海洋学会
- 页数：1
- 文件大小：40k
- 原文格式：O
- 会议级别：地方
摘要
为研究DNA条形码技术在厦门海域鱼类的分类鉴定中的可行性,随机选取了厦门海域23种鱼类样品进行COⅠ基因扩增,结果共获取23条序列,平均长度为656 bp,序列中T、C、G、A碱基的平均含量分别为:29.40%、28.10%、18.40%、24.10%。样品种内、种间、科内和目内的遗传距离(K2P)分别为:0.21%,20.50%,24.47%和25.62%,遗传距离随着分类阶元的提高而增大,种间遗传距离明显大于种内遗传距离。所选厦门海域鱼类样品仅蓝圆鯵鉴定到圆鯵属,未能鉴定到种,其余22种全部鉴定到种,表明COⅠ基因序列可以作为鱼类分类鉴定的有效DNA条形码。
In this study,we collected DNA barcodes based on CO Ⅰ sequences from 23 species of fish specimens captured in Xiamen.The mean length of the sequences was 656 base-pair and the average content of T,C,G and A were 29.4%,28.1%,18.4%and 24.1%,respectively.The average Kimura two parameter(K2P) distances within-species,genus,family and order were 0.205%,20.50%,24.47%and 25.62%,respectively.Interspecific genetic distance was much larger than intraspecific distance from composition.K2 P genetic distance values were found increasing with taxonomic level.All species,except for Decapterus maruadsi,can be differentiated by their CO Ⅰsequence.High efficiency of species identification was demonstrated in the present study by DNA barcoding,and we conclude that CO Ⅰ sequencing can be used to identify fish species.
In this study,we collected DNA barcodes based on CO Ⅰ sequences from 23 species of fish specimens captured in Xiamen.The mean length of the sequences was 656 base-pair and the average content of T,C,G and A were 29.4%,28.1%,18.4%and 24.1%,respectively.The average Kimura two parameter(K2P) distances within-species,genus,family and order were 0.205%,20.50%,24.47%and 25.62%,respectively.Interspecific genetic distance was much larger than intraspecific distance from composition.K2 P genetic distance values were found increasing with taxonomic level.All species,except for Decapterus maruadsi,can be differentiated by their CO Ⅰsequence.High efficiency of species identification was demonstrated in the present study by DNA barcoding,and we conclude that CO Ⅰ sequencing can be used to identify fish species.
引文