高效液相色谱法测定鸡肌肉中地克珠利、妥曲珠利及其代谢物的残留量
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- 英文论文题名：Determination of diclazuril,toltrazuriland its metabolites in chicken muscle by HPLC
- 论文作者：林仙军 ; 陆春波 ; 包爱情 ; 周芷锦 ; 罗成江 ; 李孝军 ; 应永飞
- 英文论文作者：LIN Xian-jun ; LU Chun-bo ; BAO Ai-qing ; ZHOU Zhi-jin ; LUO Cheng-jiang ; LI Xiao-jun ; YING Yong-fei ; Zhejiang Province Supervisory Institute of Veterinary Drug And Feed ; Zhoushan entry-exit inspection and quarantine bureau
- 年：2016
- 作者机构：浙江省兽药饲料监察所;舟山出入境检验检疫局;
- 论文关键词：高效液相色谱法 ; 地克珠利 ; 妥曲珠利 ; 代谢物 ; 鸡肌肉 ; 残留量
- 英文论文关键词：HPLC ; diclazuril ; toltrazuril ; metabolite ; chicken muscle ; residues
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：S859.84;O657.72
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：5
- 文件大小：423k
- 原文格式：O
- 会议级别：全国
摘要
建立了一种高效液相色谱法同时测定鸡肌肉中地克珠利、妥曲珠利、妥曲珠利砜及妥曲珠利亚砜残留量的方法。试样经乙腈涡旋提取,正己烷脱脂,经碱性氧化铝小柱净化,浓缩,流动相定容,高效液相色谱分离,紫外检测器检测,以保留时间定性,以峰面积定量。结果表明:该方法在浓度0.05-5μg/mL范围内线性良好,相关系数r均大于0.999。在50μg/kg-500μg/kg添加浓度范围,方法回收率在70%-110%之间,批内和批间变异系数均小于15%。方法简便、灵敏度高、稳定性好。
A method was established for simultaneously determination of diclazuril,toltrazuril,toltrazuril sulphone and toltrazuil sulphoxide in chicken muscle by HPLC.The analytes were extracted with acetonitrile,defatted with n-hexane,and then clean up by Alumina solid-phase extraction cartridges.The eluent was evaporated by nitrogen flow to dryness and dissolved the residues with mobile phase.HPLC was employed for separation and UV detector was used for detecting directly.Qualitative analysis was done by retention time and quantitative analysis by peak area respectively.The results showed that good linear relationship were obtained in the range of 0.05-5 μg/mL,with the correlation coefficients were all above 0.999.The average recoveries ranged from70%to 110%of the spiked level at 50-500 μg/kg,and the intra-assay coefficient and the inter-assay coefficient of variation were all below 15%..The method was simple,sensitive and stable.
A method was established for simultaneously determination of diclazuril,toltrazuril,toltrazuril sulphone and toltrazuil sulphoxide in chicken muscle by HPLC.The analytes were extracted with acetonitrile,defatted with n-hexane,and then clean up by Alumina solid-phase extraction cartridges.The eluent was evaporated by nitrogen flow to dryness and dissolved the residues with mobile phase.HPLC was employed for separation and UV detector was used for detecting directly.Qualitative analysis was done by retention time and quantitative analysis by peak area respectively.The results showed that good linear relationship were obtained in the range of 0.05-5 μg/mL,with the correlation coefficients were all above 0.999.The average recoveries ranged from70%to 110%of the spiked level at 50-500 μg/kg,and the intra-assay coefficient and the inter-assay coefficient of variation were all below 15%..The method was simple,sensitive and stable.
引文
[1]冯秀娟,刘英龙,王加才,等.广谱抗球虫药-妥曲珠利[J].中国禽业导刊,2006,23(1):29.
[2]中华人能共和国农业部公告235号.动物性食品中兽药最高残留限量[S].2002.
[3]施祖灏,朱良强,卢运站,等.鸡组织中地克珠利和妥曲珠利HPLC检测方法的建立[J].中国兽医学报,2009,29(1):79-81.109.
[4]郭永刚,江善祥.妥曲珠利在肉鸡组织中残留的研究[J].畜牧兽医,2007,39(7):37-39.
[5]杨丽萍,高淑霞,张秀玲,等.HPLC法检测家兔血浆中地克珠利含量[J].中国兽药杂志,2011,45(10):25-27.
[6]施祖灏,陆俊贤,葛庆联,等.高效毛细管电泳法同时检测地克珠利和妥曲珠利的含量[J].中国兽药杂志,2008,42(9).13-16.
[7]宫小明,孙军,董静,等.高效液相色谱-串联质谱法测定猪肉中阿维菌素类、地克珠利、妥曲珠利及其代谢物残留[J].色谱,2011,29(3):217-222.
[8]Mortier L,Daeseleire E,Peteghem C V.Determination of the coccidiostat diclazuril in poultry feed and meat by liquid chromatography-tandem mass spectrometry[J].Analytica Chimica Acta,2005,529:229-234.
[9]Olejnik M,Szprengier-Juszkiewicz T,Jedziniak P.Multi-res-idue confirmatory method for the determination of twelve coc-cidiostats in chicken liver using liquid chromatography tandemmass spectrometry[J]Journal of Chromatography A,2009,1216(46):8141-8148.
[2]中华人能共和国农业部公告235号.动物性食品中兽药最高残留限量[S].2002.
[3]施祖灏,朱良强,卢运站,等.鸡组织中地克珠利和妥曲珠利HPLC检测方法的建立[J].中国兽医学报,2009,29(1):79-81.109.
[4]郭永刚,江善祥.妥曲珠利在肉鸡组织中残留的研究[J].畜牧兽医,2007,39(7):37-39.
[5]杨丽萍,高淑霞,张秀玲,等.HPLC法检测家兔血浆中地克珠利含量[J].中国兽药杂志,2011,45(10):25-27.
[6]施祖灏,陆俊贤,葛庆联,等.高效毛细管电泳法同时检测地克珠利和妥曲珠利的含量[J].中国兽药杂志,2008,42(9).13-16.
[7]宫小明,孙军,董静,等.高效液相色谱-串联质谱法测定猪肉中阿维菌素类、地克珠利、妥曲珠利及其代谢物残留[J].色谱,2011,29(3):217-222.
[8]Mortier L,Daeseleire E,Peteghem C V.Determination of the coccidiostat diclazuril in poultry feed and meat by liquid chromatography-tandem mass spectrometry[J].Analytica Chimica Acta,2005,529:229-234.
[9]Olejnik M,Szprengier-Juszkiewicz T,Jedziniak P.Multi-res-idue confirmatory method for the determination of twelve coc-cidiostats in chicken liver using liquid chromatography tandemmass spectrometry[J]Journal of Chromatography A,2009,1216(46):8141-8148.