甘蔗渣培养灵芝研究和成份分析
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摘要
通过4个供试灵芝菌株生长速度、爬壁能力、菌丝密度和气生菌丝量等性状,筛选出最佳的灵芝母种培养基配方为马铃薯200g,葡萄糖20g,琼脂20g,甘蔗粉20g,蛋白胨3g,磷酸二氢钾3g,硫酸镁1.5g,维生素B2 0.1g;通过比较4个菌株在不同栽培基质上子实体干重、子实体厚度和子实体直径筛选到最适合蔗渣原料培养的菌种GL0003和GL0102,最适配方为甘蔗渣82%、啤酒糟15%、石灰粉2%、糖1%;椴木栽培灵芝和蔗渣栽培的灵芝之间总三萜差异不大,其中4种供试灵芝总三萜含量均高于10mg/g,蔗渣栽培的灵芝子实体多糖含量显著高于椴木栽培灵芝。重金属检测结果显示蔗渣栽培灵芝主要重金属元素含量低于椴木灵芝,但微量元素Mg、Zn、Se含量较高,其中有机硒含量为0.189 mg/kg。
引文
陈红香,王桂华,李家威,陆剑华,王东映,高裕锋,余构彬,李海乔,郭剑雄,2014.微波消解-电感耦合离字体质谱法测定广东蔗区甘蔗中的稀土元素.甘蔗糖业,2:60-70
金珊珊,柯斌榕,吴小平,汤坤鹏,2014.适于菌草代料栽培的杂交灵芝菌株选育.亚热带资源与环境学报,9(4):56-62
刘明香,林忠宁,陈敏健,韩海东,杨青,2011.茶枝屑代料栽培对灵芝生物转化率和质量的影响.福建农业学报,26(5):742-746
苏江滨,高俊永,黄向阳,2012.甘蔗渣的几种高值化利用研究进展.甘蔗糖业,(5):49-52
唐波,刘秀琴,2006.ICP-OES测定不同产地灵芝中重金属和有害元素的含量.中国现代中药,8(1):19-21唐杰,苗人云,谢丽源,甘炳成,黄忠乾,谭伟,王勇,彭卫红,2013.不同覆土材料中重金属含量对灵芝安全性的影响评价.西南农业学报,26(5):1910-1914
王庆福,黄清铧,李奇伟,安玉兴,梁磊,陈仲华,李锦荣,康佩姿2014.甘蔗渣啤酒糟高效转化平菇试验研究.热带农业科学,34(12):1-5
姚松君,黄生权,陈壮耀,曾庆孝,2009.超声辅助提取灵芝三萜的工艺研究.现代食品科技,(10):1220-1223Aliyu S,Bala M,2013.Brewer’s spent grain:A review of its potentials and applications.African Journal of Biotechnology,10(3):324-331
Fatmawati S,Shimizu K,Kondo R,2011.Structure-activity relationships of ganoderma acids from Ganoderma lucidum as aldose reductase inhibitors.Bioorganic medicinal chemistry letters,21(24):7295-7297
Loganathan J,Jiang J,Smith A,Jedinak A,Thyagarajansahu A,2014.The mushroom Ganoderma lucidum suppresses breast-to-lung cancer metastasis through the inhibition of pro-invasive genes.International journal of oncology,44(6):2009-2015
Pal M,Calvo A M,Terron M C,González A E,1995.Solid-state fermentation of sugarcane bagasse with Flammulina velutipes and Trametes versicolor.World Journal of Microbiology and Biotechnology,11(5):541-545Shi Y,Sun J,He H,Guo H,Zhang S,2008.Hepatoprotective effects of Ganoderma lucidum peptides against d-galactosamine-induced liver injury in mice.Journal of Ethnopharmacology,117(3):415-419
Yue G G L,Chan B C L,Han X Q,Cheng L,Wong EC,2013.Immunomodulatory activities of Ganoderma sinense polysaccharides in human immune cells.Nutrition and cancer,65(5):765-774
Zhang R Y,Hu D D,Ma X T,Li S G,Gu J G,2014.Adopting stick spawn reduced the spawn running time and improved mushroom yield and biological efficiency of Pleurotus eryngii.Scientia Horticulturae,175:156-159
周明,程薇,叶丽秀,熊光权,杜欣,何建军,杨德,陈学玲,关健,2008.不同形态棉花副产物料对灵芝生长及经济性状的影响.湖北农业科学,47(8):951-954
金珊珊,柯斌榕,吴小平,汤坤鹏,2014.适于菌草代料栽培的杂交灵芝菌株选育.亚热带资源与环境学报,9(4):56-62
刘明香,林忠宁,陈敏健,韩海东,杨青,2011.茶枝屑代料栽培对灵芝生物转化率和质量的影响.福建农业学报,26(5):742-746
苏江滨,高俊永,黄向阳,2012.甘蔗渣的几种高值化利用研究进展.甘蔗糖业,(5):49-52
唐波,刘秀琴,2006.ICP-OES测定不同产地灵芝中重金属和有害元素的含量.中国现代中药,8(1):19-21唐杰,苗人云,谢丽源,甘炳成,黄忠乾,谭伟,王勇,彭卫红,2013.不同覆土材料中重金属含量对灵芝安全性的影响评价.西南农业学报,26(5):1910-1914
王庆福,黄清铧,李奇伟,安玉兴,梁磊,陈仲华,李锦荣,康佩姿2014.甘蔗渣啤酒糟高效转化平菇试验研究.热带农业科学,34(12):1-5
姚松君,黄生权,陈壮耀,曾庆孝,2009.超声辅助提取灵芝三萜的工艺研究.现代食品科技,(10):1220-1223Aliyu S,Bala M,2013.Brewer’s spent grain:A review of its potentials and applications.African Journal of Biotechnology,10(3):324-331
Fatmawati S,Shimizu K,Kondo R,2011.Structure-activity relationships of ganoderma acids from Ganoderma lucidum as aldose reductase inhibitors.Bioorganic medicinal chemistry letters,21(24):7295-7297
Loganathan J,Jiang J,Smith A,Jedinak A,Thyagarajansahu A,2014.The mushroom Ganoderma lucidum suppresses breast-to-lung cancer metastasis through the inhibition of pro-invasive genes.International journal of oncology,44(6):2009-2015
Pal M,Calvo A M,Terron M C,González A E,1995.Solid-state fermentation of sugarcane bagasse with Flammulina velutipes and Trametes versicolor.World Journal of Microbiology and Biotechnology,11(5):541-545Shi Y,Sun J,He H,Guo H,Zhang S,2008.Hepatoprotective effects of Ganoderma lucidum peptides against d-galactosamine-induced liver injury in mice.Journal of Ethnopharmacology,117(3):415-419
Yue G G L,Chan B C L,Han X Q,Cheng L,Wong EC,2013.Immunomodulatory activities of Ganoderma sinense polysaccharides in human immune cells.Nutrition and cancer,65(5):765-774
Zhang R Y,Hu D D,Ma X T,Li S G,Gu J G,2014.Adopting stick spawn reduced the spawn running time and improved mushroom yield and biological efficiency of Pleurotus eryngii.Scientia Horticulturae,175:156-159
周明,程薇,叶丽秀,熊光权,杜欣,何建军,杨德,陈学玲,关健,2008.不同形态棉花副产物料对灵芝生长及经济性状的影响.湖北农业科学,47(8):951-954