细胞自噬在ISKNV和SCRV复制感染中的作用研究
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- 英文论文题名：Researches on the roles of autophagy in ISKNV and SCRV infection
- 论文作者：李趁 ; 付小哲 ; 林强 ; 刘礼辉 ; 梁红茹 ; 黄志斌 ; 李宁求
- 英文论文作者：LI Chen ; FU Xiaozhe ; LIN Qiang ; LIU Lihui ; LIANG Hongru ; HUANG Zhibin ; LI Ningqiu ; Pearl River Fisheries Research Institute ; Chinese Academy of Fishery Sciences ; Key Laboratory of Fishery Drug Development ; Ministry of Agriculture ; Key Laboratory of Aquatic Animal Immune Technology
- 年：2016
- 作者机构：中国水产科学研究院珠江水产研究所农业部渔用药物创制重点实验室广东省水产动物免疫技术重点实验室;上海海洋大学水产与生命学院;淡水水产健康养殖湖北省协同创新中心;
- 论文关键词：Siniperca chuatsi ; ISKNV ; SCRV ; 细胞自噬 ; LC3B ; 抗病毒效应
- 英文论文关键词：Siniperca chuatsi ; ISKNV ; SCRV ; autophagy ; LC3B ; antiviral response
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S943
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：538k
- 原文格式：D
- 会议级别：全国
摘要
细胞自噬在抗病毒感染过程中发挥着重要作用。鳜传染性脾肾坏死病毒(infectious kidney and spleen necrosis virus,ISKNV)和鳜弹状病毒(Siniperca chuatsirhabdovirus,SCRV)是对鳜养殖业危害极大的两种病毒。本实验以ISKNV和SCRV高度敏感的鳜鱼脑细胞系(CPB)为模型,通过透射电镜观察自噬相关结构,免疫印迹检测鳜微管相关蛋白1轻链3(LC3B)的转换,共聚焦显微镜观察荧光聚点等方法证实了两种病毒感染均诱导CPB细胞发生自噬。从mRNA表达、蛋白合成、以及粒子释放3个水平上对调控自噬后病毒的增殖情况进行了研究,结果表明抑制自噬能够促进SCRV增殖;而自噬对ISKNV的影响相对复杂,雷帕霉素诱导自噬后,ISKNV基因复制和蛋白合成得到促进,病毒粒子的释放却受到抑制;氯喹处理后,粒子的释放得到促进。该项研究揭示了自噬在ISKNV和SCRV感染中的作用,为病毒致病机理以及抗病毒治疗策略提供了新的视角。
Autophagy plays an important role in the host protection against virus infection. In this study, we investigated the role of autophagy in the replication ofinfectious kidney and spleen necrosis virus(ISKNV) and Siniperca chuatsi rhabdovirus(SCRV), which have caused devastating losses in Mandarin fish industry. We found that both virus infection could induce autophagy in Chinese perch brain cells(CPB), including microtubule-associated protein 1 light chain 3B II(LC3B-II) conversion, an increased accumulation of punctate GFP-LC3-expressing cells and a higher number of autophagosome- double-membrane vesicles in the cytoplasm.Furthermore, we investigated the role of autophagy in the process of ISKNV and SCRV replication by assessing mRNA and protein level as well as virus titer in the supernatant. Results showed that autophagy inhibited the replication of SCRV. While, inducing autophagy by rapamycin promoted ISKNV replication and proteins synthesis but inhibited the virus particle release. Blocking autophagosome-lysosome fusion by chloroquine(CQ) promoted virus particle release. These results demonstrated the roles of autophagy in ISKNV and SCRV, providing new insights into viral pathogenesis and antiviral treatment strategies.
Autophagy plays an important role in the host protection against virus infection. In this study, we investigated the role of autophagy in the replication ofinfectious kidney and spleen necrosis virus(ISKNV) and Siniperca chuatsi rhabdovirus(SCRV), which have caused devastating losses in Mandarin fish industry. We found that both virus infection could induce autophagy in Chinese perch brain cells(CPB), including microtubule-associated protein 1 light chain 3B II(LC3B-II) conversion, an increased accumulation of punctate GFP-LC3-expressing cells and a higher number of autophagosome- double-membrane vesicles in the cytoplasm.Furthermore, we investigated the role of autophagy in the process of ISKNV and SCRV replication by assessing mRNA and protein level as well as virus titer in the supernatant. Results showed that autophagy inhibited the replication of SCRV. While, inducing autophagy by rapamycin promoted ISKNV replication and proteins synthesis but inhibited the virus particle release. Blocking autophagosome-lysosome fusion by chloroquine(CQ) promoted virus particle release. These results demonstrated the roles of autophagy in ISKNV and SCRV, providing new insights into viral pathogenesis and antiviral treatment strategies.
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