产酸克雷伯氏菌的诱变和筛选
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- 英文论文题名：The mutagenesis and screening of Klebsiella oxytoca
- 论文作者：朱玉芹 ; 江丹 ; 方真
- 英文论文作者：Yuqin Zhu ; D Jiang ; Z Fang ; College of Life Sciences ; University of Science and Technology of China ; Institut of Chemistry Xishuangbanna Tropical Botanic Garden ; Chinese Academy of Sciences
- 年：2016
- 作者机构：中国科学院西双版纳热带植物园生物能源组;中国科学技术大学生命科学学院;
- 论文关键词：2 ; 3-丁二醇 ; 紫外诱变 ; 产酸克雷伯氏菌
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十三分会：绿色化学
- 语种：中文
- 分类号：TQ923
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十三分会 ; 绿色化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：168k
- 原文格式：D
- 会议级别：全国
摘要
2,3-丁二醇是一种极具价值的液体燃料,燃烧值为27k J/g。由于化学合成成本高、过程繁琐、不易操作,因而微生物法生产2,3-丁二醇成为研究热点。本研究旨在利用UV诱变提高产酸克雷伯氏菌的2,3-丁二醇产量。首先对产酸克雷伯氏菌进行UV诱变的条件筛选,筛选照射高度、照射时间及菌悬液浓度等条件,得出最佳诱变条件为照射高度15cm、照射时间30s、菌悬液浓度为10~6CFU/ml。接着在最适条件下对产酸克雷伯氏菌进行紫外诱变筛选出高产2,3-丁二醇的菌株,并且通过多代连续培养观测其遗传稳定性,筛选不退化的菌株保存。
2, 3-butanediol is a valuable liquid fuel and has a combustion value of 27 k J/g.Since the high cost,complicated process and difficult operation of Chemical synthesis, microbial production of 2, 3-butanediol has become a research hotspot. This study aims to utilize UV mutagenesis to improve the 2, 3-butanediol yield of Klebsiella oxytoca.Firstly,conduct the condition screening of UV mutagenesis to search the optimal exposure height 15 cm,irradiation time 30 s and Bacteria suspension concentration 10~7 CFU/ml. Then mutate the Klebsiella oxytoca under the optimal condition to screen the strain which can highly produce 2,3-butanediol.Afterwards,observe the hereditary stability of mutated strains through continous culture of many generations,so as to get unabated strain.
2, 3-butanediol is a valuable liquid fuel and has a combustion value of 27 k J/g.Since the high cost,complicated process and difficult operation of Chemical synthesis, microbial production of 2, 3-butanediol has become a research hotspot. This study aims to utilize UV mutagenesis to improve the 2, 3-butanediol yield of Klebsiella oxytoca.Firstly,conduct the condition screening of UV mutagenesis to search the optimal exposure height 15 cm,irradiation time 30 s and Bacteria suspension concentration 10~7 CFU/ml. Then mutate the Klebsiella oxytoca under the optimal condition to screen the strain which can highly produce 2,3-butanediol.Afterwards,observe the hereditary stability of mutated strains through continous culture of many generations,so as to get unabated strain.
引文
[1]蒋丽群,郭峰,方真,龙运多,张帆.化学与生物工程.2011,28(6):25
[2]赵世敏,严群,阮文权,邹华.食品与发酵工业.2008,34(3):29
[2]赵世敏,严群,阮文权,邹华.食品与发酵工业.2008,34(3):29