一种新型用于蛋白质分析的DR-PSS共价键合毛细管涂层的制备
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摘要
毛细管电泳是蛋白质分离分析一种有力手段[1,2]。为了有效抑制蛋白质在毛细管壁的吸附问题,对管壁表面进行涂层修饰是目前一种行之有效的方法[3]。本文主要介绍了利用层层自组装技术,将光敏性聚合物重氮树脂(DR)与聚苯乙烯磺酸钠(PSS)涂布到毛细管的内壁表面,从而形成一种新型抗蛋白吸附的毛细管涂层。水溶性的重氮树脂作为一种带正电的聚电解质,不但可以与二氧化硅表面的硅羟基、PSS发生静电自组装,从而在二氧化硅表面上形成非共价键合的层层自组装超薄膜,而且经过光照后,重氮树脂由于重氮基分解会引发共价交联反应,进而在二氧化硅表面上形成稳定的DR-PSS共价键合涂层。该涂层能有效抑制蛋白质的管壁吸附问题,快速、有效地分离了Lys,Cyt-c,BSA,Mb and RNase A四种蛋白质,且具有较好的稳定性和重现性。由于该方法利用层层自组装、光和热交联反应等的特性和优势,采用DR取代了毒性高、易水解的硅烷化试剂,为我们制备高质量、高性能的多种共价键合毛细管涂层提供了一种快速、简便、绿色的途径。
引文
[1]B.Yu,M.Jiao,H.Cong,X.Shu,S.Yang,A novel diazoresin/poly(N-vinyl aminobutyric acid)covalent capillary coating for the analysis of proteins by capillary electrophoresis,J.Sep.Sci.37(2014)725-730.
[2]B.Yu,W.Cui,H.Cong,M.Jiao,P.Liu,S.Yang,A novel diazoresin/polyethylene glycol covalent capillary coating for analysis of proteins by capillary electrophoresis,RSC Adv.3(2013)20010-20015.
[3]B.Yu,P.Liu,H.Cong,J.Tang,L.Zhang,Novel covalently coated diazoresin/polyvinyl alcohol capillary column for the analysis of proteins by capillary electrophoresis,Electrophoresis 33(2012)3066-3072.
[2]B.Yu,W.Cui,H.Cong,M.Jiao,P.Liu,S.Yang,A novel diazoresin/polyethylene glycol covalent capillary coating for analysis of proteins by capillary electrophoresis,RSC Adv.3(2013)20010-20015.
[3]B.Yu,P.Liu,H.Cong,J.Tang,L.Zhang,Novel covalently coated diazoresin/polyvinyl alcohol capillary column for the analysis of proteins by capillary electrophoresis,Electrophoresis 33(2012)3066-3072.