非洲菊组织培养和植株再生
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- 英文论文题名：Tissue Culture and Plant Regeneration of Gerbera jamesonii
- 论文作者：杨丽玲 ; 卢璇 ; 曹秋香 ; 范燕萍
- 英文论文作者：YANG Li-ling ; LU Xuan ; CAO Qiu-xiang ; FAN Yan-ping ; College of Forestry and Landscape Architecture ; Flower Research Center ; South China Agricultural University
- 年：2016
- 作者机构：华南农业大学林学与风景园林学院华南农业大学花卉研究中心;
- 论文关键词：非洲菊 ; 组织培养 ; 光质 ; 植物生长调节剂
- 英文论文关键词：Gerbera jamesonii ; Tissue culture ; Light quality ; Phytohormone
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.11
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：6
- 文件大小：602k
- 原文格式：O
- 会议级别：全国
摘要
非洲菊(Gerbera jamesonii)属多年生草本观赏植物,是世界十大鲜切花之一。本实验以课题组选育的非洲菊新品种‘幻彩'和‘火焰',商业品种‘高山'和‘革命红'以及课题组培育的杂交新品系HN-01为试材,进行非洲菊组织培养研究,探究适于不同非洲菊品种的最适培养基和培养条件。结果表明:最适宜非洲菊诱导愈伤并出芽的外植体是花托,‘火焰'芽诱导最适培养基是MS+BA 7.0 mg/L+KT 3.0 mg/L+NAA 0.3mg/L;‘幻彩'芽诱导最适培养基是MS+BA 7.0 mg/L+NAA 0.3 mg/L。光质对‘高山'和‘革命红'非洲菊的增殖培养具有显著影响。0.1 mg/L的NAA更适于非洲菊杂交新品系HN-01生根培养。
Gerbera jamesonii is a perennial herbaceous ornamental plant.It is one of the most important ten fresh cut flowers in the world.The tissue culture of new varieties of Gerbera which breeding in our research group G.jamesonii' Huancai',G.jamesonii ' Huoyan' and commercial varieties G.Jamesonii ' Gaoshan',G.jamesonii ' Revolution Red' and hybrid lines HN-01 were studied.The optimum culture medium and culture condition were explored in different varieties of G.jamesonii.It showed that the most suitable explanl for callus induction and Shoots Induction of Gerbera was the receptacle.The most suitable medium for the Shoots induction of ' Huoyan' was basal MS medium supplemented with BA 7.0 mg/L,KT 3.0 mg/L and NAA0.3 mg/L The most optimum medium for the Shoots induction of ' Huancai' was MS medium supplemented with BA 7.0 mg/L and NAA 0.3 mg/L Light quality plays Significant effect on the growth of G.Jamesonii ' Gaoshan' and G.jamesonii ' Revolution Red' during the proliferation.It showed that 0.1 mg/L NAA was more suitable for Gerbera hybrid lines HN-01 root culture.
Gerbera jamesonii is a perennial herbaceous ornamental plant.It is one of the most important ten fresh cut flowers in the world.The tissue culture of new varieties of Gerbera which breeding in our research group G.jamesonii' Huancai',G.jamesonii ' Huoyan' and commercial varieties G.Jamesonii ' Gaoshan',G.jamesonii ' Revolution Red' and hybrid lines HN-01 were studied.The optimum culture medium and culture condition were explored in different varieties of G.jamesonii.It showed that the most suitable explanl for callus induction and Shoots Induction of Gerbera was the receptacle.The most suitable medium for the Shoots induction of ' Huoyan' was basal MS medium supplemented with BA 7.0 mg/L,KT 3.0 mg/L and NAA0.3 mg/L The most optimum medium for the Shoots induction of ' Huancai' was MS medium supplemented with BA 7.0 mg/L and NAA 0.3 mg/L Light quality plays Significant effect on the growth of G.Jamesonii ' Gaoshan' and G.jamesonii ' Revolution Red' during the proliferation.It showed that 0.1 mg/L NAA was more suitable for Gerbera hybrid lines HN-01 root culture.
引文
1.董光威,秦新惠.2008.切花非洲菊有机生态型无土栽培技术[J].中国果菜,12-13.
2.黄济明,倪跃元,林满红.1987.非洲菊的快速繁殖[J].园艺学报,14(2):125-128.
3.高艳明,李建设,李晓娟.2006.非洲菊花托组织培养的研究[J].西北农业学报,15(4):200-202.
4.刘玉佩,黄雪琳,康吉利,等.2008.非洲菊组培快繁研究进展[J].现代农业科技,(21):77-78.
5.王爱民,肖炜,杜文雪,等.2001.光质对缕丝花试管苗生长发育的影响[J].徐州师范大学学报(自然科学版),19(4):56-58.
6.徐士清,杨世湖,倪丹,等.2002.非洲菊试管苗叶片的组培快繁[J].园艺学报,29(5):493-494.
7.杨小玲,刘书亭.2001.花卉组培快繁与产业化发展现状及前景[J].天津农业科学,7(1):1-3.
8.郑秀芳,王桔红,李名扬.2002.影响非洲菊离体培养器官分化的因素[J].江苏林业科技,29(1):29-31.
9.张素勤,邹志荣,耿广东,等.2004.培养基和植物激素对非洲菊叶片愈伤组织诱导的研究[J].西北农林科技大学学报(自然科学版),32(10):29-32.
10.张素勤,邹志荣,耿广东,等.2007.非洲菊组织培养抑制褐变现象的研究[J].贵州农业科学,35(2):56-59.
11.杨增海.1987.园艺植物组织培养[M].北京:中国农业出版社.
12.庄应强,沈玉英.2004.不同栽培基质对切花非洲菊生长和开花的影响[J].中国农学通报,20(3):173-174,186.
13.赵小丹,周广柱.2005.非洲菊无土栽培营养液组合选优[J].农村实用工程技术(温室园艺),(9):50-52.
14.赵雁鸣,王羽飞,许昌慧,等.2015.非洲菊茎尖离体培养技术研究[J].安徽农学通报,21(5):12-14.
15.张雁丽,周子发,王洁琼,等.2012.非洲菊试管苗移栽[J].中国花卉园艺,(16):20.
16.Hansen H V.1985.A taxonomic revision of the genus Perdicium(Compositae-Mutisieae).Nordic journal of botany,5(6):543-546.
17.Harding J,Huang H,Byrne T.1991.Maternal,paternal,additive,and dominance components of variance in Gerbera[J].Theoretical and Applied Genetics,82(6):756-760.
18.Kozai T,Kitaya Y,Kutota C.1995.Collected Papers on Environmental Control in Micropropagation Volume[M].Japan:Chiba University Press,591-947.
19.Kumar S,Kanwar J K.2007.Plant regeneration from cell suspensions in Gerbera jamesonii Bolus[J].Journal of fruit and ornamental plant research,15;157.
20.Kanwar J K,Kumar S.2008.In vitro propagation of Gerbera-a review[J].Horticultural Science,35(1):35-44.
21.Miyoshi K,Asakura N.1996.Callus induction,regeneration of haploid plants and chromosome doubling in ovule cultures of pot Gerbera(Gerbera jamesonii)[J].Plant Cell Reports,16(1-2):1-5.
22.Murashige T,Serpa M,Jones J B.1974.Clonal multiplication of Gerbera through tissue culture[J].HortScience,9:175-80.
23.Pierik R L M,Steegmans H H M,Marelis J J.1973.Gerbera plantlets from in vitro cultivated capitulum explants[J].Scientia Horticulturae,1(1):117-119.
24.Roberts D.R.,Walker M.A.,Thompson T.E.,et al.1989.The effects of inhibitors of polyamine and ethylene biosynthesis on senescence,ethylene production and polyamine levels in cut carnation flowers[J].Plant Cell Physiol,25:315.
2.黄济明,倪跃元,林满红.1987.非洲菊的快速繁殖[J].园艺学报,14(2):125-128.
3.高艳明,李建设,李晓娟.2006.非洲菊花托组织培养的研究[J].西北农业学报,15(4):200-202.
4.刘玉佩,黄雪琳,康吉利,等.2008.非洲菊组培快繁研究进展[J].现代农业科技,(21):77-78.
5.王爱民,肖炜,杜文雪,等.2001.光质对缕丝花试管苗生长发育的影响[J].徐州师范大学学报(自然科学版),19(4):56-58.
6.徐士清,杨世湖,倪丹,等.2002.非洲菊试管苗叶片的组培快繁[J].园艺学报,29(5):493-494.
7.杨小玲,刘书亭.2001.花卉组培快繁与产业化发展现状及前景[J].天津农业科学,7(1):1-3.
8.郑秀芳,王桔红,李名扬.2002.影响非洲菊离体培养器官分化的因素[J].江苏林业科技,29(1):29-31.
9.张素勤,邹志荣,耿广东,等.2004.培养基和植物激素对非洲菊叶片愈伤组织诱导的研究[J].西北农林科技大学学报(自然科学版),32(10):29-32.
10.张素勤,邹志荣,耿广东,等.2007.非洲菊组织培养抑制褐变现象的研究[J].贵州农业科学,35(2):56-59.
11.杨增海.1987.园艺植物组织培养[M].北京:中国农业出版社.
12.庄应强,沈玉英.2004.不同栽培基质对切花非洲菊生长和开花的影响[J].中国农学通报,20(3):173-174,186.
13.赵小丹,周广柱.2005.非洲菊无土栽培营养液组合选优[J].农村实用工程技术(温室园艺),(9):50-52.
14.赵雁鸣,王羽飞,许昌慧,等.2015.非洲菊茎尖离体培养技术研究[J].安徽农学通报,21(5):12-14.
15.张雁丽,周子发,王洁琼,等.2012.非洲菊试管苗移栽[J].中国花卉园艺,(16):20.
16.Hansen H V.1985.A taxonomic revision of the genus Perdicium(Compositae-Mutisieae).Nordic journal of botany,5(6):543-546.
17.Harding J,Huang H,Byrne T.1991.Maternal,paternal,additive,and dominance components of variance in Gerbera[J].Theoretical and Applied Genetics,82(6):756-760.
18.Kozai T,Kitaya Y,Kutota C.1995.Collected Papers on Environmental Control in Micropropagation Volume[M].Japan:Chiba University Press,591-947.
19.Kumar S,Kanwar J K.2007.Plant regeneration from cell suspensions in Gerbera jamesonii Bolus[J].Journal of fruit and ornamental plant research,15;157.
20.Kanwar J K,Kumar S.2008.In vitro propagation of Gerbera-a review[J].Horticultural Science,35(1):35-44.
21.Miyoshi K,Asakura N.1996.Callus induction,regeneration of haploid plants and chromosome doubling in ovule cultures of pot Gerbera(Gerbera jamesonii)[J].Plant Cell Reports,16(1-2):1-5.
22.Murashige T,Serpa M,Jones J B.1974.Clonal multiplication of Gerbera through tissue culture[J].HortScience,9:175-80.
23.Pierik R L M,Steegmans H H M,Marelis J J.1973.Gerbera plantlets from in vitro cultivated capitulum explants[J].Scientia Horticulturae,1(1):117-119.
24.Roberts D.R.,Walker M.A.,Thompson T.E.,et al.1989.The effects of inhibitors of polyamine and ethylene biosynthesis on senescence,ethylene production and polyamine levels in cut carnation flowers[J].Plant Cell Physiol,25:315.