15个秋石斛兰品种间杂交及种子萌发特性研究
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- 英文论文题名：Study on Hybridization and Seed Germination between 15 Varieties of Dendrobium
- 论文作者：陈和明 ; 李佐 ; 肖文芳 ; 吕复兵
- 英文论文作者：CHEN He-ming ; LI Zuo ; XIAO Wen-fang ; LU Fu-bing ; Environmental Horticulture Research Institute ; Guangdong Academy of Agricultural Sciences ; Guangdong Provincial Key Laboratory of Ornamental Plant Germplasm Innovation and Utilization
- 年：2016
- 作者机构：广东省农业科学院环境园艺研究所/广东省园林花卉种质创新综合利用重点实验室;
- 论文关键词：石斛兰 ; 杂交 ; 萌发
- 英文论文关键词：Dendrobium ; Crossedbreed ; Germination
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.31
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：6
- 文件大小：691k
- 原文格式：O
- 会议级别：全国
摘要
选用15个秋石斛兰进行不完全双列杂交,对其结果性、蒴果成熟性、蒴果大小及其重量,以及种子萌发情况进行了研究。结果表明:①不同杂交组合的结果率存在明显差异,最高达到100%,最低为0。②由于秋石斛兰品种间的差异,进行正反杂交有利于提高杂交成功率。③蒴果从授粉至采收时间高于120d,有利于蒴果内结出种子,且种子的萌发率高;若蒴果从授粉至采收时间低于90d,种子萌发较慢或极为缓慢。④蒴果的重量、长度和宽度之间经相关分析,表明果实的大小和果的重量存在的一定的关系。⑤不同杂交组合原球茎形成所需的时间差异较小,一般在15~30d膨大变绿。⑥原球茎的膨大率100%,萌发率也为100%,但原球茎的膨大率或萌发率与蒴果采收时间存在一定的关系。⑦以MS+BA 2.0mg/L+NAA 0.5mg/L+活性炭1.0g/L+蔗糖30.0g/L+椰汁100.0g/L+琼脂7.0g/L为诱导培养基,种子萌发较快且萌发率高。
15 varieties of Dendrobium were selected for incomplete diallel cross.The setting percentage,capsule mature,the capsule size and weight,seed germination were carried out during them.The results showed that;①There were significant differences in setting percentage during cultivars and the setting percentage of cross combination was 0 ~ 100%.②The reciprocal crosses will help improve the success rate of hybridization because of the differences during varieties.③The capsule than120 days from pollination to mature,the seed inside capsule formed and the seed germination rate was high,if less than 90 d,the germination of seed was relatively slow or very slow.④There was certain relationship between capsule size and weight of the capsule.⑤ The difference of corm forming time was smaller in different cross combinations between 15 d and 30 d.⑥ The expansion rate of corm was 100%,the germination rate of seed was also 100%,but there was a certain relationship between expansion rate of corm or germination rate and capsule recovery time.⑦ The seed germinated rapidly and germination rate was high on MS + BA 2.0mg/L + 0.5mg/L NAA + activated carbon 1.0g/L + sucrose 30.0g/L + 100.0g/L coconut milk +agar 7.0g/L.
15 varieties of Dendrobium were selected for incomplete diallel cross.The setting percentage,capsule mature,the capsule size and weight,seed germination were carried out during them.The results showed that;①There were significant differences in setting percentage during cultivars and the setting percentage of cross combination was 0 ~ 100%.②The reciprocal crosses will help improve the success rate of hybridization because of the differences during varieties.③The capsule than120 days from pollination to mature,the seed inside capsule formed and the seed germination rate was high,if less than 90 d,the germination of seed was relatively slow or very slow.④There was certain relationship between capsule size and weight of the capsule.⑤ The difference of corm forming time was smaller in different cross combinations between 15 d and 30 d.⑥ The expansion rate of corm was 100%,the germination rate of seed was also 100%,but there was a certain relationship between expansion rate of corm or germination rate and capsule recovery time.⑦ The seed germinated rapidly and germination rate was high on MS + BA 2.0mg/L + 0.5mg/L NAA + activated carbon 1.0g/L + sucrose 30.0g/L + 100.0g/L coconut milk +agar 7.0g/L.
引文
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2.张晓申,王慧瑜,李晓青.蝴蝶石斛的组培快繁技术研究[J].北方园艺,2007(9):193-194.
3.王云惠,陈雄庭,张秀娟.蝴蝶石斛兰高效再生体系研究[J].广东农业科学,2007(5):46-48.
4.张娟.秋石斛组织培养的研究[J].湖北林业科技,2015,44(3):20-22.
5.江秀娜,李军,詹海洋,等.解读秋石斛的四大繁殖方法[J].中国花卉盆景,2004(9):38.
6.卢思聪.秋石斛及其栽培[J].中国花卉盆景,1999(7):6-7.
7.武荣花,李振坚,王雁.秋石斛品种及其温室栽培[J].农业工程技术(温室园艺),2007(1):34-35.
8.杨志娟,陈冠铭,柯用春,等.热带洋兰秋石斛的主要病害及其防治[J].现代园艺,2013(11):66-67.
9.黄志明,林庆良,余慧敏.蝴蝶石斛兰工厂化育苗技术的研究[J].莆田学院学报,2002,9(3):22-26.
10.符岸军,李娟玲,李劲松,等.石斛兰“绿色星辰×出水芙蓉”杂交种子无菌萌发与组培快繁研究[J].安徽农业科学,2011,39(5):2577-2580.
11.陈娜,方炎明,程磊.大苞鞘石斛种子萌发过程中形态变化研究[J].安徽农业科学,2013,41(3):1038-1040.
12.王伟,靳晓翠,金荷仙,等.大苞鞘石斛种子离体萌发培养[J].种子,2011,30(4):45-49.
13.霍丽丽,潘会堂,张启翔.肿节石斛的无菌播种和快速繁殖[J].植物生理学通讯,2010,46(12):1269-1270.
14.龚建英,王华新,陈宝玲,等.3种观赏石斛蒴果特征及无菌播种萌发的影响因素[J].江苏农业科学,2015,43(1):184-186.