氧化纳米碳球在SALDI-TOF MS中的应用
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- 英文论文题名：Oxidized carbon nanospheres as surface assistant matrix for highly efficient detection of small molecules by SALDI-TOF MS
- 论文作者：李鑫 ; 徐桂菊 ; 张红燕 ; 刘胜菊 ; 牛欢 ; 彭佳喜 ; 吴仁安
- 英文论文作者：Xin Li ; Guiju Xu ; Shengju Liu ; Hongyan Zhang ; Huan Niu ; Jiaxi Peng ; Ren'an Wu ; Dalian Institute of Chemical Physics ; Chinese Academy of Sciences (CAS) ; University of Chinese Academy of Sciences
- 年：2016
- 作者机构：中国科学院大连化学物理研究所;中国科学院大学;
- 论文关键词：氧化碳球 ; 基质 ; 小分子化合物 ; 表面辅助激光/解析吸飞行时间质谱
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第四十三分会：质谱分析
- 语种：中文
- 分类号：O657.63;O613.71;TB383.1
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第四十三分会 ; 质谱分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：360k
- 原文格式：D
- 会议级别：全国
摘要
基质辅助激光解吸飞行时间质谱(MALDI-TOF MS)因其高通量、高灵敏度等优点而成为蛋白、多肽及代谢组学研究中的有力工具~([1-2])。然而,常用的CHCA、DHB等有机基质在分子量低于500 Da时存在严重的背景干扰,限制了MALDIMS在小分子化合物检测中的应用~([3])。近年来,研究者~([4])提出使用无机纳米粒子或者纳米结构替代有机基质(SALDI-TOFMS),可以有效避免基质背景干扰,用于低分子量化合物分析。本文制备了形貌规整的纳米氧化碳球,使其作为MALDI基质成功运用于多种结构的小分子化合物分析。相比于石墨烯、碳纳米管等碳基材料,氧化纳米碳球制备方法简单、成本低廉。并且其良好的水分散性以及均匀的靶板分布形态,使得质谱检测的背景干扰小,重复性好,灵敏度高。
Oxidized carbon nanospheres(OCS) were prepared and demonstrated to own continuous π-conjugated structures,uniform nanosize and excellent water-dispersibility.Owing to their unique properties,the OCS was for the first time applied as an assistant matrix in Surface-assisted laser desorption/ionization time-of-flight mass spectroscopy(SALDI-TOF MS) for small molecules analysis.Compared with conventional organic matrix and other carbon-based matrices,low background interference and enhanced signal intensities were obtained by OCS matrix The good reproducibility and excellent salt tolerance of OCS matrix were also investigated.
Oxidized carbon nanospheres(OCS) were prepared and demonstrated to own continuous π-conjugated structures,uniform nanosize and excellent water-dispersibility.Owing to their unique properties,the OCS was for the first time applied as an assistant matrix in Surface-assisted laser desorption/ionization time-of-flight mass spectroscopy(SALDI-TOF MS) for small molecules analysis.Compared with conventional organic matrix and other carbon-based matrices,low background interference and enhanced signal intensities were obtained by OCS matrix The good reproducibility and excellent salt tolerance of OCS matrix were also investigated.
引文
[1]Karas,M.;Hillenkamp,F.Anal.Chem.1988,60,2299.
[2]Aebersold,R.;Mann,M.Nature 2003,422,198.
[3]Liu,Q.;Cheng,M.;Wang,J.;Jiang,G.Chem.Eur.J.2015,21,5594
[4]Lin,Z.;Zheng,J.;Lin,G.;Tang,Z.;Cai.Z.W.Anal.Chem.2015,87,8005
[2]Aebersold,R.;Mann,M.Nature 2003,422,198.
[3]Liu,Q.;Cheng,M.;Wang,J.;Jiang,G.Chem.Eur.J.2015,21,5594
[4]Lin,Z.;Zheng,J.;Lin,G.;Tang,Z.;Cai.Z.W.Anal.Chem.2015,87,8005