氨基硅烷化磁纳米颗粒固定化果胶酶改善烟草浸膏品质
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- 英文论文题名：Pectinase immobilized by aminosilanized magnetic nanoparticles for improving quality of tobacco extract
- 论文作者：颜克亮 ; 陈虹 ; 王文峰 ; 胡巍耀 ; 凌军 ; 陈微 ; 余洪波
- 英文论文作者：YAN Keliang ; CHEN Hong ; WANG Wenfeng ; HU Weiyao ; LING Jun ; CHEN Wei ; YU Hongbo ; Technology Center ; China Tobacco Yunnan Industrial Co. ; Ltd ; College of Life Science and Technology ; Huazhong University of Science and Technology
- 年：2016
- 作者机构：云南中烟工业有限责任公司技术中心;华中科技大学生命科学与技术学院;
- 论文关键词：烟草浸膏 ; 氨基硅烷化磁纳米颗粒 ; 固定化果胶酶 ; 还原糖 ; 果胶水解
- 英文论文关键词：tobacco extract ; amino-silanized magnetic nanoparticles ; immobilized pectinase ; reducing sugars ; hydrolysis of pectin
- 会议召开时间：2016-12-01
- 会议录名称：中国烟草学会2016年度优秀论文汇编——烟草工业主题
- 语种：中文
- 分类号：Q814.2;TS47
- 学会代码：ZGYG
- 学会名称：中国烟草学会
- 页数：8
- 文件大小：550k
- 原文格式：D
摘要
为降低烟草浸膏中的不利成分,首次利用氨基硅烷化磁纳米颗粒固定化果胶酶制剂处理烟草浸膏,研究了酶处理条件对浸膏中果胶和蛋白含量的影响,并对处理后的烟草浸膏在再造烟叶中的应用效果进行感官评价。结果显示:①固定化果胶酶蛋白及酶活回收率分别为44.44%和40.86%;②固定化酶处理的最佳条件为:温度50℃、pH 3.5、酶浓度4.44U/mL、反应时间24 h;③利用固定化酶处理叶膏和梗膏,果胶水解率分别达到84.04%和39.26%,浸膏中的酶蛋白可通过磁性分离完全去除;④将处理后的烟草浸膏用于再造烟叶制备,吸味明显改善。该方法可有效降低烟草浸膏中果胶含量,并除去残留酶蛋白,可改善烟草浸膏品质。
Immobilized pectinase was used for the first time to deal with tobacco extract so as to reduce adverse constituents in tobacco extract.Different conditions that can affect concentration of pectin and protein in tobacco extract have been studied.Application verification of tobacco extracts which have been treated with immobilized pectinase has been done.Results showed that immobilized pectinase had a protein recovery rate of 44.44%and a catalytic activity recovery rate of 40.86%.The optimized condition for immobilized pectinase was as below:temperature 50℃,the pH 3.5,enzyme activity 4.44 U/mL and enzymolysis time 24 h.After treated by immobilized pectinase,the pectin hydrolysis rate of tobacco extract was 84.04%(leaf) and 39.26%(stem),and the immobilized pectinase could be removed from tobacco extract under the power of magnetic force.Reconstituted tobacco made by tobacco extract which had been treated by immobilized pectinase had better taste than reconstituted tobacco made by tobacco extract which had no treatment.Pectin concentration of tobacco extract can be obviously reduced after being treated by pectinase immobilized by amino-silanized magnetic nanoparticles.Immobilized pectinase can be removed,thus improving and the quality of tobacco extract.
Immobilized pectinase was used for the first time to deal with tobacco extract so as to reduce adverse constituents in tobacco extract.Different conditions that can affect concentration of pectin and protein in tobacco extract have been studied.Application verification of tobacco extracts which have been treated with immobilized pectinase has been done.Results showed that immobilized pectinase had a protein recovery rate of 44.44%and a catalytic activity recovery rate of 40.86%.The optimized condition for immobilized pectinase was as below:temperature 50℃,the pH 3.5,enzyme activity 4.44 U/mL and enzymolysis time 24 h.After treated by immobilized pectinase,the pectin hydrolysis rate of tobacco extract was 84.04%(leaf) and 39.26%(stem),and the immobilized pectinase could be removed from tobacco extract under the power of magnetic force.Reconstituted tobacco made by tobacco extract which had been treated by immobilized pectinase had better taste than reconstituted tobacco made by tobacco extract which had no treatment.Pectin concentration of tobacco extract can be obviously reduced after being treated by pectinase immobilized by amino-silanized magnetic nanoparticles.Immobilized pectinase can be removed,thus improving and the quality of tobacco extract.
引文
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[11]安丽娟,李兆强,徐娓,等.超顺磁性高分子微球的制备与表征[J].高等学校化学学报,2005,26(2):366-369.An Lijuan,Li Zhaoqiang,Xu Wei,et al.Preparation and Characterization of Superparamagnetism Polymer Microsphere[J].Chemical Journal of Chinese Universities,2005,26(2):366-369.(in Chinese)
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[13]刘耀飞,常纪恒,于川芳.烟梗中果胶的酶法降解[J].烟草科技,2013,(8):40-44.Liu Yaofei,Chang Jiheng,Yu Chuanfang.Enzymatic Degradation of Pectin in Tobacco Stem[J].Tobacco Science&Technology,2013,(8):40-44.(in Chinese)
[14]NY 82.11—1988,果汁测定方法果胶的测定[S].NY 82.11—1988,The determination of pectin in fruit juice[s].(in Chinese)
[15]史玮,孙莹,徐振斌.凯氏定氮法测定粮食蛋白质含量方法研究[J].粮食科技与经济,2013,38(5):31-32.Shi Wei,Sun Ying,Xu Zhenbin.Study of Protein Content Detection of Grain Using Kjeldahl Determination Method[J].Grain Science and Technology and Economy,2013,38(5):31-32.(in Chinese)
[16]章平泉,金殿明,杜秀敏,等.自动凯氏定氮仪测定烟草及其制品中的总氮[J].烟草科技,2011,(3):43-45.Zhang Pingquan,Jin Dianming,Du Xiumin,et al.Determination of Total Nitrogen in Tobacco Products by Automatic Kjekdahl Nitrogen Analyzer[J].Tobacco Science&Technology,2011,(3):43-45.(in Chinese)
[17]冯斌,任志强,屈晶苗,等.3-氨丙基三乙氧基硅烷表面修饰的磁性Fe_3O_4纳米粒子合成与表征[J].化工新型材料,2009,36(12):26-29.Feng Bin,Ren Zhiqiang,Qu Jingmiao,et al.Preparation and Characterization of(3-Aminapropyl)Triethoxysilane Coated Magnetite Nanoparticles[J].New Chemical Materials,2009,36(12):26-29.(in Chinese)
[18]李杨,江连洲,李丹丹,等.氨基硅烷修饰的磁纳米粒子固定化碱性蛋白酶[J].食品科学,2012,33(9):202-205.Li Yang,Jiang Lianzhou,Li Dandan,et al.Immobilization of Alkaline Protease with Magnetic Nanoparticles Modified by Amino-Silane[J].Food Science,2012,33(9):202-205.(in Chinese)
[19]杨可可,杨婉身,王显祥,等.硅烷化氨基磁性粒子的制备及其固定化木瓜蛋白酶的研究[J].四川农业大学学报,2008,26(2):135-140.Yang Keke,Yang Wanshen,Wang Xianxiang,et al.Preparation of Amino Group Coated Magnetic Microspheres by Silanization and its Study on Immobilizing Papain[J].Journal of Sichuan Agricultural University,2008,26(2):135-140.(in Chinese)
[20]沈昊宇,赵永纲,胡美琴,等.高分子聚羧酸-纳米Fe_3O_4磁性复合颗粒的制备及其对水中对羟基苯甲酸酯类化合物的吸附作用[J].复合材料学报,2009,26(4):68-73.Shen Haoyu,Zhao Yonggang,Hu Meiqin,et al.Preparation and Adsorption Properties of Polycarboxylate NanoFe_3O_4 Magnetic Composite Particles[J].Acta Materiae Compositae Sinica,2009,26(4):68-73.(in Chinese)
[2]吴亦集,沈光林,陶红,等.造纸法再造烟叶原料的加酶萃取[J].烟草科技,2011,(7):33-36.Wu Yiji,Shen Guanglin,Tao Hong,et al.Extraction of Tobacco Materials for Paper-making Process Reconstituted Tobacco in Presence of Enzyme Mixture[J].Tobacco Science&Technology,2011,(7):33-36.(in Chinese)
[3]李琳,韦杰,王豹祥,等.复合酶处理废次烟末制备烟草浸膏[J].湖北农业科学,2012,51(9):1803-1807.Li Lin,Wei Jie,Wang Baoxiang,et al.Preparation of Tobacco Wastes Extracts with Complex Enzymes[J].Hubei Agricultural Sciences,2012,51(9):1803-1807.(in Chinese)
[4]隋颖,张立平.功能化磁性纳米颗粒固定化脂肪酶研究[J].应用化工,2013,42(11):1978-1980.Sui Ying,Zhang Liping.Study on Lipase Immobilization on Functional Magnetic Nanoparticles[J].Applied chemical industry,2013,42(11):1978-1980.(in Chinese)
[5]丁文武,孙铭珍,曹汝毅,等.纳米磁性微粒固定化脂肪氧合酶的制备及其酶活研究[J].粮油食品科技,2015,23(2):79-82.Ding Wenwu,Sun Mingzhen,Cao Ruyi,et al.Preparation of Magnetic Nanoparticles Immobilized Lipoxygenase and its Activity[J].Science and Technology of Cereals;Oils and Foods,2015,23(2):79-82.(in Chinese)
[6]陈陶声,居乃琥,陈石根,等.固定化酶理论与应用[M].北京:轻工业出版社,1987:98-102.Chen Taosheng,Ju Naihu,Chen Shigen,et al.Theory and Application of Immobilized Enzyme[M].Bei Jing:China Light Industry Press,1987:98-102.(in Chinese)
[7]陈明洁,张汉昌,关志荣.化学共沉淀法制备Fe_3O_4纳米颗粒的结构和磁性能研究[J].材料导报:纳米与新材料专辑,2008(3):94-96.Chen Mingjie,Zhang Hanchang,Guan Zhirong.Research on Structural and Magnetic Features of Fe_3O_4 Nanoparticles Synthesized by Chemical Co-precipitation Method[J].Materials Review:Nano and New Materials,2008(3):94-96.(in Chinese)
[8]Xu L,Kim M J,Kim K D,et al.Surface modified Fe_3O_4nanoparticles as a protein delivery vehicle[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2009,350(1):8-12.
[9]胡玮,娄兆文.四氧化三铁磁性纳米微粒表面的氨基化修饰[J].化学研究,2013(2):144-148.Hu Wei,Lou Zhaowen.Amination Surface-Modification of Ferriferous Oxide Magnetic Nanoparticles[J].Chemical Research,2013(2):144-148.(in Chinese)
[10]阮贵华,刘玉花,陈正毅,等.Fe_3O_4磁性纳米粒子固定化胰蛋白酶[J].桂林理工大学学报,2013,35(4):569-574.Ruan Guihua,Liu Yuhua,Chen Zhengyi,et al.Tryptase immobilized by Fe_3O_4 magnetic nanoparticles[J].Journal of Guilin University of Technology,2013,35(4):569-574.(in Chinese)
[11]安丽娟,李兆强,徐娓,等.超顺磁性高分子微球的制备与表征[J].高等学校化学学报,2005,26(2):366-369.An Lijuan,Li Zhaoqiang,Xu Wei,et al.Preparation and Characterization of Superparamagnetism Polymer Microsphere[J].Chemical Journal of Chinese Universities,2005,26(2):366-369.(in Chinese)
[12]周正红,贾宗剑.利用DNS定糖法快速测定烟草中水溶性总糖的研究[J].烟草科技,1998,(1):26-29.Zhou Zhenghong,Jia Zongjian.Study on Rapid Mesuring Soluble Sugar by DNS Method[J].Tobacco Science&Technology,1998,(1):26-29.(in Chinese)
[13]刘耀飞,常纪恒,于川芳.烟梗中果胶的酶法降解[J].烟草科技,2013,(8):40-44.Liu Yaofei,Chang Jiheng,Yu Chuanfang.Enzymatic Degradation of Pectin in Tobacco Stem[J].Tobacco Science&Technology,2013,(8):40-44.(in Chinese)
[14]NY 82.11—1988,果汁测定方法果胶的测定[S].NY 82.11—1988,The determination of pectin in fruit juice[s].(in Chinese)
[15]史玮,孙莹,徐振斌.凯氏定氮法测定粮食蛋白质含量方法研究[J].粮食科技与经济,2013,38(5):31-32.Shi Wei,Sun Ying,Xu Zhenbin.Study of Protein Content Detection of Grain Using Kjeldahl Determination Method[J].Grain Science and Technology and Economy,2013,38(5):31-32.(in Chinese)
[16]章平泉,金殿明,杜秀敏,等.自动凯氏定氮仪测定烟草及其制品中的总氮[J].烟草科技,2011,(3):43-45.Zhang Pingquan,Jin Dianming,Du Xiumin,et al.Determination of Total Nitrogen in Tobacco Products by Automatic Kjekdahl Nitrogen Analyzer[J].Tobacco Science&Technology,2011,(3):43-45.(in Chinese)
[17]冯斌,任志强,屈晶苗,等.3-氨丙基三乙氧基硅烷表面修饰的磁性Fe_3O_4纳米粒子合成与表征[J].化工新型材料,2009,36(12):26-29.Feng Bin,Ren Zhiqiang,Qu Jingmiao,et al.Preparation and Characterization of(3-Aminapropyl)Triethoxysilane Coated Magnetite Nanoparticles[J].New Chemical Materials,2009,36(12):26-29.(in Chinese)
[18]李杨,江连洲,李丹丹,等.氨基硅烷修饰的磁纳米粒子固定化碱性蛋白酶[J].食品科学,2012,33(9):202-205.Li Yang,Jiang Lianzhou,Li Dandan,et al.Immobilization of Alkaline Protease with Magnetic Nanoparticles Modified by Amino-Silane[J].Food Science,2012,33(9):202-205.(in Chinese)
[19]杨可可,杨婉身,王显祥,等.硅烷化氨基磁性粒子的制备及其固定化木瓜蛋白酶的研究[J].四川农业大学学报,2008,26(2):135-140.Yang Keke,Yang Wanshen,Wang Xianxiang,et al.Preparation of Amino Group Coated Magnetic Microspheres by Silanization and its Study on Immobilizing Papain[J].Journal of Sichuan Agricultural University,2008,26(2):135-140.(in Chinese)
[20]沈昊宇,赵永纲,胡美琴,等.高分子聚羧酸-纳米Fe_3O_4磁性复合颗粒的制备及其对水中对羟基苯甲酸酯类化合物的吸附作用[J].复合材料学报,2009,26(4):68-73.Shen Haoyu,Zhao Yonggang,Hu Meiqin,et al.Preparation and Adsorption Properties of Polycarboxylate NanoFe_3O_4 Magnetic Composite Particles[J].Acta Materiae Compositae Sinica,2009,26(4):68-73.(in Chinese)