表达狂犬病病毒SRV9株糖蛋白重组慢病毒载体的构建及重组病毒的筛选鉴定
详细信息 查看官网全文
- 英文论文题名：Construction of Recombinant Lentivirus Expressing Glycoprotein of Rabies Virus SRV9 Strain
- 论文作者：陈远媚 ; 陈强 ; 向蓉 ; 陈晶 ; 黄元 ; 黄忠 ; 向华 ; 王晓虎
- 英文论文作者：Chen Yuanmei ; Chen Qiang ; Xiang Rong ; Chen Jing ; Huan Yuan ; Huang Zhong ; Xiang Hua ; Wang Xiaohu ; Institute of Animal Health ; Guangdong Academy of Agricultural Sciences ; Veterinary Public Health Laboratory of Guangdong Province ; Veterinary station of Jiangkou Town
- 年：2016
- 作者机构：广东省农业科学院动物卫生研究所广东省兽医公共卫生公共实验室;广西壮族自治区桂平市江口镇水产畜牧兽医站;
- 论文关键词：狂犬病病毒 ; 弱毒株SRV9 ; 糖蛋白 ; 重组慢病毒
- 英文论文关键词：Rabies virus ; Attenuated strain SRV9 ; Glycoprotein ; Recombinant lentivirus
- 会议召开时间：2016-06-14
- 会议录名称：第25届广东省科技进步活动月畜牧兽医学术与科技创新发展大会论文集
- 语种：中文
- 分类号：S852.65
- 学会代码：GDKL
- 会议名称：第25届广东省科技进步活动月畜牧兽医学术与科技创新发展大会
- 会议地点：中国广东广州
- 主办单位：广东省畜牧兽医学会
- 学会名称：广东省科学技术协会科技交流部
- 页数：4
- 文件大小：654k
- 原文格式：O
- 会议级别：地方
摘要
狂犬病病毒(Rabies virus,RABV)糖蛋白(G1ycoprotein,GP)是唯一刺激机体产生针对狂犬病病毒中和抗体的抗原,是基因工程狂犬病疫苗研究的主要靶蛋白。本研究使用Gateway技术,设计特异性引物扩增狂犬病病毒弱毒株SRV9的糖蛋白基因,先后经过BP反应和LR反应、转化,获得经测序正确的pLv-SRG载体质粒,与pPACKH1-1-1-Gag、pPACKH1-Rev、pVSV-G2-1包装质粒共转染293T细胞,包装重组病毒并鉴定,滴度为3×10~8TU/mL。本实验获得表达狂犬病病毒弱毒株SRV9糖蛋白基因的重组慢病毒,为开展下一步的疫苗研究奠定基础。
Rabies virus(RABV) glycoprotein(GP) is the only antigen protein that stimulates bodies to produce antibodies against rabies virus.So,it is the major target of rabies genetic engineering vaccine research.Using Gateway technology,specific primers were designed to amplify the rabies virus glycoprotein gene of attenuated strain SRV9.Then we obtained the correct pLv-SRG vector plasmid by BP and LR reactions,conversion and sequencing.The packaging plasmids of pPACKH1-1-1-Gag,pPACKH1-Rev,pVSV-G2-1 were prepared and co-transfected into 293 T cells.We got the correct recombinant virus after identification.The titer of the recombinant virus is 3×10~8 TU/mL.The attenuated strain SRV9 of rabies virus glycoprotein gene was expressed in therecombinant lentivirus.It's a useful foundation for following vaccine research.
Rabies virus(RABV) glycoprotein(GP) is the only antigen protein that stimulates bodies to produce antibodies against rabies virus.So,it is the major target of rabies genetic engineering vaccine research.Using Gateway technology,specific primers were designed to amplify the rabies virus glycoprotein gene of attenuated strain SRV9.Then we obtained the correct pLv-SRG vector plasmid by BP and LR reactions,conversion and sequencing.The packaging plasmids of pPACKH1-1-1-Gag,pPACKH1-Rev,pVSV-G2-1 were prepared and co-transfected into 293 T cells.We got the correct recombinant virus after identification.The titer of the recombinant virus is 3×10~8 TU/mL.The attenuated strain SRV9 of rabies virus glycoprotein gene was expressed in therecombinant lentivirus.It's a useful foundation for following vaccine research.
引文