摘要
为了构建HA2-基因的新功能型重组乳酸菌并检测其表达产物的免疫原性,本实验通过PCR将来源于禽流感病毒的血凝素基因HA2插入锚定表达载体pSIP-409-pgsA'中,构建重组质粒pSIP-409-pgsA'-HA2,并将其电转到植物乳杆菌NC8中,通过Western-blot检测目的蛋白的表达情况。结果表明:pSIP-409-pgsA'-HA2蛋白成功表达,并且具有反应原性,为后期实验奠定了基础。
By means of PCR to make the hemagglutinin gene HA2-,which from bird flu virus plug in anhored expression vector pSIP-409-pgsA',The experiment aims to constructe the recombinant plasmid and then eletroprate it into lactobacillus NC8 in order to make a new features recombinant lactobacillus of HA2-and detect the immunogenic of expression product.The expression of the target protein can be detected through western-blot.The result shows that the protein pSIP-409-pgsA'-HA2 has successfully expressed,then to provide with reactogenicity,which lays the foundation of the later experiments.
引文
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