新功能型乳酸菌NC8-pSIP-409-pgsA'-HA2的构建
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- 英文论文题名：Construction of the Lactic acid bacteria of expressive Rck protein
- 论文作者：王棋 ; 石昊林 ; 王占楠 ; 黄科谚 ; 姚心茹 ; 王成宇 ; 杨桂连 ; 姜延龙 ; 王春凤
- 英文论文作者：WANG qi ; SHI hao-lin ; WANG zhan-nan ; HUANG ke-yan ; YAO Xin-ru ; WANG Cheng-yu ; YANG Gui-lian ; JIANG Yan-long ; WANG Chun-feng ; Jilin Provincial Engineering Research Center of Animal Probiotics ; College of Animal Science and Technology ; Jilin Agricultural University
- 年：2016
- 作者机构：吉林农业大学动物科学技术学院吉林省动物微生态制剂工程研究中心;
- 论文关键词：pSIP-409-pgsA' ; HA2 ; 植物乳杆菌 ; 新功能型乳酸菌
- 英文论文关键词：pSIP-409-pgsA' ; HA2 ; Lactobacillus plantarum ; New functional lactic acid bacteria
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：Q933
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：65k
- 原文格式：O
- 会议级别：全国
摘要
为了构建HA2-基因的新功能型重组乳酸菌并检测其表达产物的免疫原性,本实验通过PCR将来源于禽流感病毒的血凝素基因HA2插入锚定表达载体pSIP-409-pgsA'中,构建重组质粒pSIP-409-pgsA'-HA2,并将其电转到植物乳杆菌NC8中,通过Western-blot检测目的蛋白的表达情况。结果表明:pSIP-409-pgsA'-HA2蛋白成功表达,并且具有反应原性,为后期实验奠定了基础。
By means of PCR to make the hemagglutinin gene HA2-,which from bird flu virus plug in anhored expression vector pSIP-409-pgsA',The experiment aims to constructe the recombinant plasmid and then eletroprate it into lactobacillus NC8 in order to make a new features recombinant lactobacillus of HA2-and detect the immunogenic of expression product.The expression of the target protein can be detected through western-blot.The result shows that the protein pSIP-409-pgsA'-HA2 has successfully expressed,then to provide with reactogenicity,which lays the foundation of the later experiments.
By means of PCR to make the hemagglutinin gene HA2-,which from bird flu virus plug in anhored expression vector pSIP-409-pgsA',The experiment aims to constructe the recombinant plasmid and then eletroprate it into lactobacillus NC8 in order to make a new features recombinant lactobacillus of HA2-and detect the immunogenic of expression product.The expression of the target protein can be detected through western-blot.The result shows that the protein pSIP-409-pgsA'-HA2 has successfully expressed,then to provide with reactogenicity,which lays the foundation of the later experiments.
引文
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[8]佟盼盼.产肠毒素大肠杆菌FaeG与树突状细胞诱导肽融合重组乳酸菌的制备及免疫效果的评估[D].吉林长春,吉林农业大学,2012.
[2]SIDDIQUI FJ,RABBANI F,HASAN R,et al.Typhoid fever in children:some epidemiological considerations from Karachi,Pakistan.Int J Infect Dis,2011;10(3):215-222.
[3]Fuller T,Ducatez M,Njabo K,et al.Avian influenza surveillance in Central and West Africa,2010-2014[J].Epidemiol Infect,2014,22:1-8.
[4]Gao R,CaoB,HuY,etal.Human infection with a novel avian-origin influenza A(H7N9)virusf J].New England Journal of Medicine,2013,368(20):1888-1897.
[5]J.Rahn,D.Hoffmann,T.C.Harder,M.Beer,etal.Vaccines against influenza A viruses in poultry and swine:Status and future developments[J].Vaccine,2015,33:2414-2424.
[6]郝凤奇,李景梅,杨桂连,等.乳酸菌Nisin诱导表达载体的构建和鉴定[J].中国预防兽医学报,2012,34(8):624-628.
[7]郭衍冰.树突状细胞诱导肽重组NDV HN基因乳酸菌的制备及免疫效果检测[D].吉林长春,吉林农业大学,2014.
[8]佟盼盼.产肠毒素大肠杆菌FaeG与树突状细胞诱导肽融合重组乳酸菌的制备及免疫效果的评估[D].吉林长春,吉林农业大学,2012.