基于差示离心沉降法分析纳米颗粒形状与界面生物吸附
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- 英文论文题名：Analysis of shape and surface biomolecule adsorption of nanoparticles using differential centrifugal sedimentation
- 论文作者：王瑞敏 ; 陈岚 ; 葛广路
- 英文论文作者：Ruimin Wang ; Lan Chen ; Guanglu Ge ; National Center for Nanoscience and Technology
- 年：2016
- 作者机构：国家纳米科学中心;
- 论文关键词：差示离心沉降法 ; 纳米颗粒 ; 金纳米棒 ; 长径比 ; 蛋白质吸附
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十一分会：胶体与界面化学
- 语种：中文
- 分类号：TB383.1;O647.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十一分会 ; 胶体与界面化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：134k
- 原文格式：D
- 会议级别：全国
摘要
利用离心等方法将多分散纳米颗粒按粒径和形状进行有效分离,需要对纳米颗粒在离心场中的运动进行精确的分析。对非球形颗粒来说,挑战来自其形状依赖的阻力系数,及粒子取向和布朗运动的不确定性。我们使用差示离心沉降法测量等体积和等直径的金纳米棒沉降系数,考察长径比的影响,并与文献中基于球形度和受力分析的模型进行对比。该方法在同时检测不同尺寸金纳米颗粒的表面蛋白质吸附方面也有独特的优势。
Sorting polydispersed colloidal nanoparticles into fractions utilizing their differential movement dictated by their size and shape requires quantitative understanding of particle motion.For non-spherical nanoparticles,the analysis of their sedimentation velocity is challenging because the drag force is affected by particle size and geometry in a complex way,plus the uncertainty of particle orientation and Brownian motion.Herein we report our effort in the aspect of particle geometry,namely the influence of the volume/mass and aspect ratio of colloidal gold nanorods(GNRs) on their settlement behavior,investigated by differential centrifugal sedimentation(DCS).Our results serve as a testing ground for various models describing non-spherical nanoparticles moving in a centrifugal field based on sphericity and force analysis,and point to further refinement.We will also discuss the usage of DCS in simultaneous monitoring of protein adsorption onto gold nanoparticles with different sizes in a mixture.
Sorting polydispersed colloidal nanoparticles into fractions utilizing their differential movement dictated by their size and shape requires quantitative understanding of particle motion.For non-spherical nanoparticles,the analysis of their sedimentation velocity is challenging because the drag force is affected by particle size and geometry in a complex way,plus the uncertainty of particle orientation and Brownian motion.Herein we report our effort in the aspect of particle geometry,namely the influence of the volume/mass and aspect ratio of colloidal gold nanorods(GNRs) on their settlement behavior,investigated by differential centrifugal sedimentation(DCS).Our results serve as a testing ground for various models describing non-spherical nanoparticles moving in a centrifugal field based on sphericity and force analysis,and point to further refinement.We will also discuss the usage of DCS in simultaneous monitoring of protein adsorption onto gold nanoparticles with different sizes in a mixture.
引文