Au/Fe_3O_4纳米粒子的合成、修饰及其在高敏C反应蛋白检测中的应用
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- 英文论文题名：The synthesis and modification of Au/Fe_3O_4 nanoparticles and its application in the detection of human high sensitive C-reactive protein
- 论文作者：高琴 ; 彭明丽 ; 崔亚丽 ; 陈超
- 英文论文作者：Qin Gao ; Mingli Peng ; Yali Cu ; Chao Chen ; College of Chemistry and Material Science ; Northwest University ; National Engineering Research Center for Miniaturized Detection Systems Northwest University
- 年：2016
- 作者机构：西北大学化学与材料科学学院;西北大学国家微检测系统工程技术研究中心;
- 论文关键词：Au/Fe3O4 ; 氧化 ; 高敏C反应蛋白 ; 甘油磷酸胆碱 ; 化学发光免疫分析
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十八分会：纳米生物效应与纳米药物化学
- 语种：中文
- 分类号：TB383.1;O657.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十八分会 ; 纳米生物效应与纳米药物化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：254k
- 原文格式：D
- 会议级别：全国
摘要
Au/Fe_3O_4纳米复合材料由于独特的物理化学性质及其在生物医学领域的应用而成为纳米材料研究的热点之一。~([1])本研究在利用高温热解可控制备花瓣型Au/Fe_3O_4纳米粒子后,釆用NaIO_4氧化法将纳米表面油酸中氧化为端羧基基团,从而实现单分散的Au/Fe_3O_4纳米粒子的水相转移。利用透射电镜(TEM),X-射线粉末衍射(X-RD)及傅立叶红外光谱(FT-IR)等手段的表征结果表明:Au/Fe_3O_4纳米粒子的粒径约为35nm;粒径分布均一,在正己烷中具有良好分散性和稳定性。水相转移后的Au/Fe_3O_4纳米粒子在粒径、形状、晶形上都没有变化,在水中具有良好分散性和稳定性(图A所示)。进一步通过1-(3-二甲氨基丙基)-3-环己基碳二亚胺(EDC)偶联将高敏C反应蛋白(CRP)抗体包被在纳米粒子表面、再经过甘油磷酸胆碱(GPC)封闭后,~([2])利用双抗体夹心法进行磁酶联免疫化学发光,实现了CRP高灵敏检测(图B所示)。
Flower-like Au/Fe_3O_4 nanoparticles are synthesized by thermal decomposition of Fe(CO)_5 in the presence of Au nanoparticles followed by aqueous phase transfer with oxidation of oleic acid by NaIO_4 to give the carboxyl group on the surface.The obtained Au/Fe_3O_4 nanoparticles dispersed in water solution was characterized and was proved to be 35 nm in size and exhibits good dispersion in water and the morphology and crystallinity have no changes during the phase transfer. The hydrophilic Au/Fe_3O_4 was activated by EDC and couple anti-CRP antibodies to form Au/Fe_3O_4-antibody conjugated, then the surface on the nanoparticles was blocked with GPC to reduce or eliminate nonspecific adsorption in the detection of CRP in serum. A sandwich-type chemiluminescent immunoassay was established, indicating higher sensitivity with GPC as blocking reagent and Au/Fe_3O_4 nanoparticles as magnetic carrier.
Flower-like Au/Fe_3O_4 nanoparticles are synthesized by thermal decomposition of Fe(CO)_5 in the presence of Au nanoparticles followed by aqueous phase transfer with oxidation of oleic acid by NaIO_4 to give the carboxyl group on the surface.The obtained Au/Fe_3O_4 nanoparticles dispersed in water solution was characterized and was proved to be 35 nm in size and exhibits good dispersion in water and the morphology and crystallinity have no changes during the phase transfer. The hydrophilic Au/Fe_3O_4 was activated by EDC and couple anti-CRP antibodies to form Au/Fe_3O_4-antibody conjugated, then the surface on the nanoparticles was blocked with GPC to reduce or eliminate nonspecific adsorption in the detection of CRP in serum. A sandwich-type chemiluminescent immunoassay was established, indicating higher sensitivity with GPC as blocking reagent and Au/Fe_3O_4 nanoparticles as magnetic carrier.
引文
[1]Leung KC,Xuan S,Zhu X,et al.,Chem.Soc.Rev.2012,41:1911-1928.
[2]J.B.Schlenoff.,Langmuir.2014,30:9625-9636.
[2]J.B.Schlenoff.,Langmuir.2014,30:9625-9636.