高温胁迫及外源水杨酸对大丽花抗氧化酶活性的影响
详细信息 查看官网全文
- 英文论文题名：Effects of Exogenous Salicylic Acid on Antioxidant Enzyme Activity under High Temperature Stress in Dahlia pinnata
- 论文作者：赵川乐 ; 张萍萍 ; 孔周阳 ; 李坚 ; 管华 ; 谈建中
- 英文论文作者：ZHAO Chuan-le ; ZHANG Ping-ping ; KONG Zhou-yang ; LI Jian ; GUAN Hua ; TAN Jian-zhong ; Department of Horticulture ; College of Architecture ; Soochow University
- 年：2016
- 作者机构：苏州大学建筑学院园艺系;
- 论文关键词：大丽花 ; 组织培养 ; 高温胁迫 ; 水杨酸 ; 抗氧化酶
- 英文论文关键词：Dahlia ; Tissue culture ; High temperature stress ; Salicylic acid ; Antioxidant enzyme
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S682.261
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：4
- 文件大小：538k
- 原文格式：O
- 会议级别：全国
摘要
为了探讨大丽花高温胁迫及外源水杨酸作用的生理机制,以大丽花组培苗为供试材料,在培养基中添加不同浓度的水杨酸(SA),分析高温胁迫对大丽花抗氧化酶活性变化。结果表明,在35℃/30℃(昼/夜)高温胁迫下,大丽花SOD活性持续增加,在胁迫第8d达到最大值;POD活性在胁迫初期几乎未受影响,到第12d时才显著上升;CAT活性虽然持续下降,但变化幅度较小。培养基中添加0.05mmol·L~(-1)的SA可以促进大丽花组培苗的生长与生根,并显著提高了大丽花SOD、POD和CAT的酶活性。说明适宜浓度的外源SA可以调节大丽花的抗氧化酶活性,并能促进大丽花的生长发育,从而有利于提高大丽花对高温胁迫的耐受性。
To investigate the physiological response of dahlia to high temperature and exogenous salicylic acid(SA),the shoots cultured in vitro was used as test materials.Different concentration of SA was added in the medium to analyze the change of antioxidant enzyme activity under high temperature stress in dahlia.The results showed that under high temperature stress(35℃/30℃,day/night),the SOD activity continued to grow and reached the maximum value in the 8~(th) day,and the POD activity did not change significantly in the early stage of stress until the 12' day.Meanwhile the CAT activity continued to decrease,but didn't change much.0.05 mmol · L~(-1) of SA added in the medium could promote the growth and rooting,and increase the SOD,POD and CAT activities of dahlia in vitro.It showed that suitable concentration of SA could adjust the antioxidant enzyme activity and promote the growth,thus to improve tolerance to high temperature stress in dahlia
To investigate the physiological response of dahlia to high temperature and exogenous salicylic acid(SA),the shoots cultured in vitro was used as test materials.Different concentration of SA was added in the medium to analyze the change of antioxidant enzyme activity under high temperature stress in dahlia.The results showed that under high temperature stress(35℃/30℃,day/night),the SOD activity continued to grow and reached the maximum value in the 8~(th) day,and the POD activity did not change significantly in the early stage of stress until the 12' day.Meanwhile the CAT activity continued to decrease,but didn't change much.0.05 mmol · L~(-1) of SA added in the medium could promote the growth and rooting,and increase the SOD,POD and CAT activities of dahlia in vitro.It showed that suitable concentration of SA could adjust the antioxidant enzyme activity and promote the growth,thus to improve tolerance to high temperature stress in dahlia
引文
1.曹淑红,李宁毅.水杨酸对高温胁迫下百日草幼苗耐热性的影响[J].沈阳农业大学学报,2014(1):91-94.
2.陈秋明,尹慧,李晓艳,等.高温胁迫下外源水杨酸对百合抗氧化系统的影响[J].中国农业大学学报,2008,13(2):44-48.
3.Freeman J L,Garcia D,Kim D,et al.Constitutively elevated salicylic acid signals glutathione-mediated nickel tolerance in Thlaspi nickel hyperaccumulators[J].Plant Physiology,2005,137(3):1082-1091.
4.高俊凤.植物生理学实验指导[M].西安:世界图书出版公司,2000,194-196.
5.Gutierrez-Coronado M A,Trejo-Lopez C,Larque-Saavedra A.Effects of salicylic acid on the growth of roots and shoots in soybean[J].Plant Physiology&Biochemistry,1998,36(8):563-565.
6.Idrees M,Naeem M,Aftab T,et al.Salicylic acid mitigates salinity stress by improving antioxidant defence system and enhances vincristine and vinblastine alkaloids production in periwinkle[Catharanthus roseus(L)G.Don][J].Acta Physiologiae Plantaram,2011,33(3):987-999.
7.Janda TG,Szalai IT,Padi E.Hydroponic treatment with salicylic acid decrease the effects of chilling injury in mazie(lea Mays L)plants[J].Planta,1999,208:175-180.
8.Larkindale J,Hall J D,Knight MR,et al.Heat stress phenotypes of arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance[J].Plant Physiology,2005,138(2):882-897.
9.李合生,孙群,赵世杰,等.植物生理生化试验原理与技术[M].北京:高等教育出版社,2000.
10.刘安成,王庆,庞长民.我国大丽花园艺学研究进展[J].北方园艺,2010,(11):225-228.
11.刘建福,王明元,唐源江,等.水杨酸和一氧化氮对姜黄生长及次生代谢产物的影响[J].园艺学报,2015,42(4):741-750.
12.Malaray J,Carr J P,K lessig D F et al.Salicylic acid:a likely endogenous signal in the resistance response of tobacco to viral infection.Sci,1990,250:1002-1004.
13.Mariana R,Vicente,Javier P.Salicylic acid beyond defence;its role in plant growth and development[J].Experimental Botany,2011,62(10):3321-3338.
14.Munne-Bosch S,Penuelas J.Photo-and antioxidative protection,and a role for salicylic acid during drought and recovery in field grown Phillyrea angustifolia plants[J].Planta2003,217:758-766.
15.Vicente MRS,Plasencia J.Salicylic acid beyond defence;its role in plant growth and development J].Journal of Experimental Botany,2011,62(10):3321-3338.
16.万正林,罗庆熙,李立志.水杨酸诱导番茄幼苗抗高温效果[J].中国蔬菜,2009(24):36-42.
17.王大平,李艳.水杨酸对常绿欧洲荚蓬扦插枝条生根的影响[J].贵州农业科学,2012,40(12):80-81.
18.王定景,司庆永,龚宁,等.高温胁迫下外源水杨酸对金线兰抗氧化酶活性的影响[J].贵州农业科学,2012,40(5);39-42.
19.Wang L J,Fan L,Loescher W,et al.Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves[J].Bmc Plant Biology,2010,10;34(3);34.
20.徐彩平.南林895杨组培苗耐盐性及耐盐机制的研究[D].南京:南京林业大学,2012.
21.杨华庚,陈慧娟.高温胁迫对蝴蝶兰幼苗叶片形态和生理特性的影响[J].中国农学通报,2009,25(11):123-127.
22.杨永花,李正平,李万祥,等.甘肃大丽花品种资源及应用[J].北方园艺,1996,(3):44-45.
23.周艳丽,李金英,王秋月,等.高温胁迫下水杨酸对黄瓜幼苗生理特性的影响[J].北方园艺,2010,(24):44-46.
2.陈秋明,尹慧,李晓艳,等.高温胁迫下外源水杨酸对百合抗氧化系统的影响[J].中国农业大学学报,2008,13(2):44-48.
3.Freeman J L,Garcia D,Kim D,et al.Constitutively elevated salicylic acid signals glutathione-mediated nickel tolerance in Thlaspi nickel hyperaccumulators[J].Plant Physiology,2005,137(3):1082-1091.
4.高俊凤.植物生理学实验指导[M].西安:世界图书出版公司,2000,194-196.
5.Gutierrez-Coronado M A,Trejo-Lopez C,Larque-Saavedra A.Effects of salicylic acid on the growth of roots and shoots in soybean[J].Plant Physiology&Biochemistry,1998,36(8):563-565.
6.Idrees M,Naeem M,Aftab T,et al.Salicylic acid mitigates salinity stress by improving antioxidant defence system and enhances vincristine and vinblastine alkaloids production in periwinkle[Catharanthus roseus(L)G.Don][J].Acta Physiologiae Plantaram,2011,33(3):987-999.
7.Janda TG,Szalai IT,Padi E.Hydroponic treatment with salicylic acid decrease the effects of chilling injury in mazie(lea Mays L)plants[J].Planta,1999,208:175-180.
8.Larkindale J,Hall J D,Knight MR,et al.Heat stress phenotypes of arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance[J].Plant Physiology,2005,138(2):882-897.
9.李合生,孙群,赵世杰,等.植物生理生化试验原理与技术[M].北京:高等教育出版社,2000.
10.刘安成,王庆,庞长民.我国大丽花园艺学研究进展[J].北方园艺,2010,(11):225-228.
11.刘建福,王明元,唐源江,等.水杨酸和一氧化氮对姜黄生长及次生代谢产物的影响[J].园艺学报,2015,42(4):741-750.
12.Malaray J,Carr J P,K lessig D F et al.Salicylic acid:a likely endogenous signal in the resistance response of tobacco to viral infection.Sci,1990,250:1002-1004.
13.Mariana R,Vicente,Javier P.Salicylic acid beyond defence;its role in plant growth and development[J].Experimental Botany,2011,62(10):3321-3338.
14.Munne-Bosch S,Penuelas J.Photo-and antioxidative protection,and a role for salicylic acid during drought and recovery in field grown Phillyrea angustifolia plants[J].Planta2003,217:758-766.
15.Vicente MRS,Plasencia J.Salicylic acid beyond defence;its role in plant growth and development J].Journal of Experimental Botany,2011,62(10):3321-3338.
16.万正林,罗庆熙,李立志.水杨酸诱导番茄幼苗抗高温效果[J].中国蔬菜,2009(24):36-42.
17.王大平,李艳.水杨酸对常绿欧洲荚蓬扦插枝条生根的影响[J].贵州农业科学,2012,40(12):80-81.
18.王定景,司庆永,龚宁,等.高温胁迫下外源水杨酸对金线兰抗氧化酶活性的影响[J].贵州农业科学,2012,40(5);39-42.
19.Wang L J,Fan L,Loescher W,et al.Salicylic acid alleviates decreases in photosynthesis under heat stress and accelerates recovery in grapevine leaves[J].Bmc Plant Biology,2010,10;34(3);34.
20.徐彩平.南林895杨组培苗耐盐性及耐盐机制的研究[D].南京:南京林业大学,2012.
21.杨华庚,陈慧娟.高温胁迫对蝴蝶兰幼苗叶片形态和生理特性的影响[J].中国农学通报,2009,25(11):123-127.
22.杨永花,李正平,李万祥,等.甘肃大丽花品种资源及应用[J].北方园艺,1996,(3):44-45.
23.周艳丽,李金英,王秋月,等.高温胁迫下水杨酸对黄瓜幼苗生理特性的影响[J].北方园艺,2010,(24):44-46.