呋喃西林的主要代谢产物氨基脲对雌性斑马鱼的抗雌激素效应及其机制的研究
详细信息 查看官网全文
- 英文论文题名：Anti-estrogenic effect of the maker metabolite of nitrofurazone——semicarbazide in female zebrafish(Danio rerio) and its potential mechanisms
- 论文作者：于淼 ; 张晓娜 ; 郭琳琳 ; 田华 ; 王蔚 ; 汝少国
- 英文论文作者：Miao Yu ; Xiaona Zhang ; Linlin Guo ; Hua Tian ; Wei Wang ; Shaoguo Ru ; College of Marine Life Sciences ; Ocean University of China
- 年：2016
- 作者机构：中国海洋大学海洋生命学院;
- 论文关键词：氨基脲 ; 17β-雌二醇 ; 生殖轴线 ; γ-氨基丁酸 ; 瘦素
- 英文论文关键词：Semicarbazide ; 17β-estradiol ; reproductive axis ; γ-aminobutyric acid ; leptin
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S948
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：577k
- 原文格式：D
- 会议级别：全国
摘要
氨基脲是水产养殖兽药呋喃西林的主要代谢产物,具有多种毒性效应。已有研究表明,氨基脲暴露可抑制雌性斑马鱼卵黄原蛋白以及雌激素受体的转录,具有抗雌激素效应。为进一步探讨氨基脲的抗雌激素效应机制,本研究采用半静态暴露实验,以1~1000μg/L的氨基脲暴露性成熟的雌性斑马鱼96h和28d,采用酶联免疫的方法检测了雌鱼血浆中17β-雌二醇(E_2)水平,用实时定量PCR的方法检测了生殖轴线中的相关基因、γ-氨基丁酸支路相关酶基因、瘦素基因的转录水平,同时观察了卵巢的显微结构。研究结果表明氨基脲暴露96h及28d后,血浆E_2水平明显下降,卵巢中卵母细胞的成熟度降低,说明氨基脲是通过降低生物体内源E_2水平来发挥其抗雌激素效应的。而氨基脲导致E_2水平降低则与生殖轴线中关键基因的转录水平显著下调有关。此外氨基脲对γ-氨基丁酸合成的抑制以及对瘦素基因转录的激活也是导致E_2水平降低的重要因素。本研究的结果为具有氨基脲类似毒性的物质开展抗雌激素效应机制研究提供了一个新的借鉴。
Semicarbazide(SMC), the marker residue for the banned veterinary antibiotic used in aquaculture, has various toxic effects. It was reported SMC exposure inhibited the transcription of hepatic vitellogenin and estrogen receptors in female zebrafish(Danio rerio), suggesting that it had anti-estrogenic properties. In order to elucidate the mechanisms underlying these, we exposed female zebrafish to SMC and used enzyme-linked immunosorbent assays to examine plasma17β-estradiol(E_2) levels. Gonad histology was analyzed and the mRNA expression of genes involved in the reproductive axis, the γ-aminobutyric acid(GABA) shunt, and leptin was quantified by real-time PCR. Zebrafish were exposed to 1~1000 μg/L SMC in a semi-static system for 96 hours or 28 days. Plasma E_2 levels were significantly decreased and ovarian maturation was inhibited by SMC, suggesting that its anti-estrogenic effect was exerted by reducing endogenous E_2 levels. This was likely due to the general depression of the gene transcriptional levels along the reproductive axis. In addition, SMC-mediated inhibition of GABA synthesis and stimulation of leptin gene transcription made contributions to the decrease of plasma E_2 levels
Semicarbazide(SMC), the marker residue for the banned veterinary antibiotic used in aquaculture, has various toxic effects. It was reported SMC exposure inhibited the transcription of hepatic vitellogenin and estrogen receptors in female zebrafish(Danio rerio), suggesting that it had anti-estrogenic properties. In order to elucidate the mechanisms underlying these, we exposed female zebrafish to SMC and used enzyme-linked immunosorbent assays to examine plasma17β-estradiol(E_2) levels. Gonad histology was analyzed and the mRNA expression of genes involved in the reproductive axis, the γ-aminobutyric acid(GABA) shunt, and leptin was quantified by real-time PCR. Zebrafish were exposed to 1~1000 μg/L SMC in a semi-static system for 96 hours or 28 days. Plasma E_2 levels were significantly decreased and ovarian maturation was inhibited by SMC, suggesting that its anti-estrogenic effect was exerted by reducing endogenous E_2 levels. This was likely due to the general depression of the gene transcriptional levels along the reproductive axis. In addition, SMC-mediated inhibition of GABA synthesis and stimulation of leptin gene transcription made contributions to the decrease of plasma E_2 levels
引文