生物样本辐照用~7Li离子束流特性诊断研究
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- 英文论文题名：Diagnostic study of ~7Li ions beam qualities for biological samples irradiation application
- 论文作者：张小玲 ; 隋丽 ; 孔福全
- 英文论文作者：ZHANG Xiao-ling ; SUI Li ; KONG Fu-quan ; Department of Nuclear Physics ; China Institute of Atomic Energy
- 年：2015
- 作者机构：中国原子能科学研究院核物理研究所;
- 论文关键词：~7Li离子 ; 高LET辐射 ; 辐射生物学 ; 束斑均匀性 ; 注量准确性
- 英文论文关键词：~7Li ions ; high LET radiation ; radiobiology ; beam uniformity ; fluence accuracy
- 会议召开时间：2015-09-21
- 会议录名称：中国核科学技术进展报告（第四卷）——中国核学会2015年学术年会论文集第7册（计算物理分卷、核物理分卷、粒子加速器分卷、核聚变与等离子体物理分卷、脉冲功率技术及其应用分卷、辐射物理分卷）
- 语种：中文
- 分类号：O571.6
- 学会代码：EGVD
- 会议名称：中国核学会2015年学术年会
- 会议地点：中国四川绵阳
- 主办单位：中国核学会
- 学会名称：中国核学会
- 页数：6
- 文件大小：454k
- 原文格式：O
- 会议级别：全国
摘要
~7Li离子属于高LET辐射,是辐射生物学基础研究中使用的重离子射线之一,它是硼中子俘获治疗癌症中起关键作用的离子。与低LET辐射相比,高LET辐射通过直接作用于生物的靶分子DNA,而诱发更为复杂、严重、不易修复的DNA损伤,如双链断裂和非双链断裂集簇性损伤等,从而引起细胞凋亡、细胞周期改变、细胞衰老和死亡、癌变和变异等生物学效应。因此,建立辐射生物学专用实验装置和相应的束流诊断方法,获得束流品质满足生物样本辐照要求的离子束,是开展~7Li离子束致生物大分子或细胞的辐射生物学效应研究的重要前提。本研究在北京HI-13串列加速器R20支线末端的生物用新终端上进行,运用不同的探测方法对串列加速器引出的43 MeV~7Li离子束流的稳定性、束斑大小和均匀性、注量的准确性进行测量和评估,实验结果表明:在4.2×10~4~1.5×10~5particles/cm~2/s注量率范围内,两种不同类型闪烁体探测器测得注量率变化呈线性关系,束流稳定性良好;均匀性好于90%的最大束斑面积为5.0 cm×5.0 cm;运用闪烁体探测器监测的~7Li离子注量与CR39固体核径迹探测器测得的绝对注量的误差小于10%。束流特性满足辐射生物学实验要求。
~7Li ions with high linear energy transfer(LET) have been used in radiobiological research,which is key particle in Boron Neutron Capture Therapy.In present study,the new terminal had been established for radiobiological application,located at the end of R20 branch beam line of HI-13 tandem accelerator.The beam qualities of ~7Li ions with the energy 43 MeV generated by HI-13 tandem accelerator,including beam stability,spot size,uniformity and particle fluence accuracy were measured using different detection methods.The results showed that the flux measured with different detectors had a good linear relationship as particle flux ranged from 4.2×10~4~1.5×10~5particles/cm~2/s,beam uniformity was over 90%at 5 cm×5 cm area,and particle fluence accuracy was better than 90%.All results showed that the beam qualities of ~7Li ions finely met the basic requirements for radiation biological experiment.
~7Li ions with high linear energy transfer(LET) have been used in radiobiological research,which is key particle in Boron Neutron Capture Therapy.In present study,the new terminal had been established for radiobiological application,located at the end of R20 branch beam line of HI-13 tandem accelerator.The beam qualities of ~7Li ions with the energy 43 MeV generated by HI-13 tandem accelerator,including beam stability,spot size,uniformity and particle fluence accuracy were measured using different detection methods.The results showed that the flux measured with different detectors had a good linear relationship as particle flux ranged from 4.2×10~4~1.5×10~5particles/cm~2/s,beam uniformity was over 90%at 5 cm×5 cm area,and particle fluence accuracy was better than 90%.All results showed that the beam qualities of ~7Li ions finely met the basic requirements for radiation biological experiment.
引文
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[11]隋丽,等.~7Li和~(12)C离子致DNA链断裂的研究[J].高能物理与核物理,2004,28(10):1126-1130.
[12]Sui Li,et al.Atomic force microscopy measurement of DNA fragment induced by heavy ions[J].Chinese Physics Letters,2005,22(4):1010-1013.
[13]KONG Fuquan,et al.The DNA concentration effect on DNA radiation damage induced by~7Li ions andγrays[J].Chinese Science Bulletin,2008,53(18):2758-2763.
[14]孔福全,等.辐射致DNA损伤中DNA浓度的影响[J].科学通报,2007,52(24):2826-2831.
[15]Slobodan Devic.Radiochromic film dosimetry:Past,present,and future[J].European Journal of Medical Physics,2011,27:122-134.
[16]Li Zhao,et al.Gafchromic EBT film dosimetry in proton beams[J].Physics in Medicine and Biology,2010,55:291-301.
[17]Daniel Kirby,et al.LET dependence of Gaf Chromic films and an ion chamber in low-energy proton dosimetry[J].Physics in Medicine and Biology,2010,55:417-413.
[2]隋丽,等.高LET的~7Li离子致DNA损伤的直接和间接作用研究[J].核技术,2007,30(4):250-254.
[3]张晓敏,等.硼中子俘获治疗技术的研究现状[J].国外医学,放射医学核医学分册,2004,28(4):188-191.
[4]戴中颖,等.HIRFL浅层肿瘤治疗终端治癌碳离子束物理特性的测量[J].高能物理与核物理,2006,30(9):920-924.
[5]徐辉辉,等.不同LET辐射致DNA集簇性损伤的研究[J].原子核物理评论,2010,27(4):469-473.
[6]Rupak Pathak,et al.Cell killing,nuclear damage and apoptosis in Chinese hamster V79 cells after irradiation with heavy-ion beams of~(16)O,~(12)C and~7Li[J].Mutation Research,2007,632:58-68.
[7]Rupak Pathak,et al.Genotoxic effects in M5 cells and Chinese hamster V79 cells after exposure to~7Li-beam(LET=60 keV/μm)and correlation of their survival dynamics to nuclear damages and cell death[J].Mutation Research,2007,628:56-66.
[8]Geetanjali Pujari,et al.The influence of reduced glutathione on chromosome damage induced by X-rays or heavy ion beams of different LETs and on the interaction of DNA lesions induced by radiations and bleomycin[J].Mutation Research,2010,696:154-159.
[9]Juliann G Kiang,et al.Radiation Combined Injury:DNA Damage,Apoptosis,and Autophagy[J].Adaptive Medicine,2010,2(1):1-10.
[10]KONG Fuquan,et al.Analysis of length distribution of short DNA fragments induced by~7Li ions using the random-breakage model[J].Chinese Science Bulletin,2005,50(9):841-844.
[11]隋丽,等.~7Li和~(12)C离子致DNA链断裂的研究[J].高能物理与核物理,2004,28(10):1126-1130.
[12]Sui Li,et al.Atomic force microscopy measurement of DNA fragment induced by heavy ions[J].Chinese Physics Letters,2005,22(4):1010-1013.
[13]KONG Fuquan,et al.The DNA concentration effect on DNA radiation damage induced by~7Li ions andγrays[J].Chinese Science Bulletin,2008,53(18):2758-2763.
[14]孔福全,等.辐射致DNA损伤中DNA浓度的影响[J].科学通报,2007,52(24):2826-2831.
[15]Slobodan Devic.Radiochromic film dosimetry:Past,present,and future[J].European Journal of Medical Physics,2011,27:122-134.
[16]Li Zhao,et al.Gafchromic EBT film dosimetry in proton beams[J].Physics in Medicine and Biology,2010,55:291-301.
[17]Daniel Kirby,et al.LET dependence of Gaf Chromic films and an ion chamber in low-energy proton dosimetry[J].Physics in Medicine and Biology,2010,55:417-413.