DNA甲基化多位点检测
详细信息 查看官网全文
- 英文论文题名：Detection of multiplexed methylcytosine sites
- 论文作者：饶爽 ; 陈玉琪 ; 杜宇昊 ; 周翔
- 英文论文作者：Shuang Rao ; Yuqi Chen ; Yuhao Du ; Xiang Zhou ; Department of Chemistry ; University of Wuhan
- 年：2016
- 作者机构：武汉大学化学与分子科学学院;
- 论文关键词：MLPA ; DNA methylation
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十八分会：化学生物学
- 语种：中文
- 分类号：Q523
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十八分会 ; 化学生物学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：2
- 文件大小：230k
- 原文格式：D
- 会议级别：全国
摘要
DNA的甲基化是一种重要的表观遗传修饰方式,它能在不改变DNA序列的前提下控制遗传性状。DNA的甲基化有三种类型,在高等真核生物中只存在胞嘧啶对5-甲基胞嘧啶的转变。DNA甲基化异常会导致一些遗传疾病和肿瘤的发生,DNA的甲基化水平是肿瘤的生物学指标,对DNA的甲基化的检测有着极为重要的医学意义。目前检测方法众多,各有其优缺点和针对性。传统的甲基化特异性多连接依赖性探针扩增法(MS-MLPA)具有需样本量少、可分析大量混合样本,一次检测多个靶基因中CpG位点的甲基化情况等优点,但探针连接位点受限制性内切酶的限制。本论文的研究目的是在传统甲基化检测方法的基础上做出改进,设计一种基于MLPA的多位点甲基化检测方法。
The methylation status of multiplexed methylcytosine sites can be simultaneously monitored by ligation-depended PCR assay.The ability of quantitative detection of multiplexed sites in one PCR reaction makes it a good choice for detecting methylation at both CpG and non-CpG sites for research and diagnosis of disease compared with others.The assay can determine as low as 20 aM methylated DNA and has been successfully applied to the genomic DNA sample derived from cancer cell lines.
The methylation status of multiplexed methylcytosine sites can be simultaneously monitored by ligation-depended PCR assay.The ability of quantitative detection of multiplexed sites in one PCR reaction makes it a good choice for detecting methylation at both CpG and non-CpG sites for research and diagnosis of disease compared with others.The assay can determine as low as 20 aM methylated DNA and has been successfully applied to the genomic DNA sample derived from cancer cell lines.
引文
(1)Bird,A.,Genes&development 2002,16(1),6-21.
(2)Li,E.;Beard,C.;Jaenisch,R.,Nature 1993,366(6453),362-5.
(2)Li,E.;Beard,C.;Jaenisch,R.,Nature 1993,366(6453),362-5.