新型静电采样器收集酵母菌气溶胶的实验研究
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- 英文论文题名：Experimental Study of C.albicans-aerosols collection with a novel Electrostatic Sampler
- 论文作者：杜艳艳 ; 徐羽贞 ; 郑超 ; 刘振 ; 黄逸凡 ; 闫克平
- 英文论文作者：DU Yanyan ; XU Yuzhen ; ZHENG Chao ; LIU Zhen ; HUANG Yifan ; YAN Keping ; College of Chemical and Biological Engineering ; Zhejiang University ; Key Laboratory of Biomass Chemical Engineering (Zhejiang University) ; Ministry of Education ; Zhejiang Institute of Medical Device
- 年：2015
- 作者机构：浙江大学化学工程与生物工程学院;浙江大学生物质化工教育部重点实验室;浙江省医疗器械研究所;
- 论文关键词：静电采样器 ; 气溶胶 ; 收集效率
- 英文论文关键词：electrostatic sampler ; aerosol ; precipitation efficiency
- 会议召开时间：2015-08-12
- 会议录名称：静电放电：从地面新技术应用到空间卫星安全防护—中国物理学会第二十届全国静电学术会议论文集
- 语种：中文
- 分类号：O648.18;X831
- 学会代码：WLJD
- 会议名称：静电放电 ; 从地面新技术应用到空间卫星安全防护—中国物理学会第二十届全国静电学术会议
- 会议地点：中国河北石家庄
- 主办单位：中国物理学会静电专业委员会
- 学会名称：中国物理学会静电专业委员会
- 页数：5
- 文件大小：578k
- 原文格式：D
- 会议级别：全国
摘要
为评价自制新型微生物气溶胶静电采样器的性能,以酵母菌气溶胶为对象开展了实验研究。通过TK-3微生物气溶胶发生器产生酵母菌气溶胶,使用新型静电采样器收集气溶胶,并用表面涂布计数法检测收集到的微生物浓度,与AGI-30液体采样器收集到的微生物浓度进行比较。与AGI-30采样器相比,静电采样器具有较好的收集效果,对酵母菌气溶胶的收集效果可达AGI-30采样器的10.8倍。新型静电采样器可以收集到酵母菌气溶胶,并可以通过改进提高收集效果。
Experimental studies on C.albicans-aerosols were carried out,which are for the evaluation of a novel electrostatic sampler performance.A TK-3 collision nebulizer was utilized to generate the C.albicans-aerosols,and collect aerosols by the novel electrostatic sampler.Detecting the concentration of microorganisms by surface coating and counting method.The performance of biological collecting efficiency of novel electrostatic sampler was compared with traditional microbial impactor AGI-30 operated in parallel.Comparison with the tradition microbial impactor AGI-30,novel electrostatic sampler was more suitable for bioaerosols precipitation.When the charging voltage was+5k V,the maxium concentration of culturable bioaerosols were collected as10.8times higher than AGI-30 sampled for C.albicans-aerosols.Novel electrostatic sampler is suitable for bioaerosols precipitation,and can improve the collection efficiency by adjusting electric parameters.
Experimental studies on C.albicans-aerosols were carried out,which are for the evaluation of a novel electrostatic sampler performance.A TK-3 collision nebulizer was utilized to generate the C.albicans-aerosols,and collect aerosols by the novel electrostatic sampler.Detecting the concentration of microorganisms by surface coating and counting method.The performance of biological collecting efficiency of novel electrostatic sampler was compared with traditional microbial impactor AGI-30 operated in parallel.Comparison with the tradition microbial impactor AGI-30,novel electrostatic sampler was more suitable for bioaerosols precipitation.When the charging voltage was+5k V,the maxium concentration of culturable bioaerosols were collected as10.8times higher than AGI-30 sampled for C.albicans-aerosols.Novel electrostatic sampler is suitable for bioaerosols precipitation,and can improve the collection efficiency by adjusting electric parameters.
引文
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[9]甄世祺.新型静电场采样器采集流感病毒研究[J].江苏预防医学,2010,21(3):1-3
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[2]潘湛,王勤熙.气溶胶病毒富集与检测研究进展[J].温州医学院学报,2008,38(6):579-581.
[3]Tania PR,Swee YL,Carolina B,et al.Offsite exposure to respirable aerosols produced during the Disk-Incorporation of class Bbiosolids[J].Environment Engineering,2007,133(10);987-994
[4]钱乐.常见空气微生物采样器研究[J].江苏预防医学,2012,23(4):49-51
[5]郁庆福.现代卫生微生物学[M].北京:人民卫生出版社,1995:384-402.
[6]于玺华,车凤翔.现代空气微生物学及采检鉴技术[M].北京:军事医学科学出版社,1998:87-174.
[7]Yao MS,Mainelis G.Utilization of naturalele ctrical charges on airborne microorganismsfor their collection by electrostatic means.Jo-urna l of Aerosol Science,2006,37(4):513-527
[8]Mainelis G,Adhikari A,Willeke K,et a1.Collection of airbome microorganisms by a new electrostatic precipitator[J].Journal of Aerosol Science,2002,33(10):1417-1432.
[9]甄世祺.新型静电场采样器采集流感病毒研究[J].江苏预防医学,2010,21(3):1-3
[10]赵智大.高电压技术[M].2版.中国电力出版社,2008:8-17
[11]Grinshpun SA,Chang CW,Nevalainen A,et al.Inlet characteristics of bioaerosol samplers.Journal of Aerosol Science,1994,25(8):1503-1522
[12]于玺华.现代空气微生物学[M].第1版.北京:人民军医出版社,2002:[13]Mainelis G.Collection of Airborne Microorganisms by Electrostatic Precipitation.Ae-r osol Science and Technology,1999,30(2):127-144