二氢香豆素抑制蜂房哈夫尼菌群体感应和生物膜形成
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- 英文论文题名：Dihydrocoumarin inhibits quorum sensing and biofilm formation of Hafnia alvei
- 论文作者：江枫 ; 张公亮 ; 王佳莹 ; 侯红漫
- 英文论文作者：Jiang Feng ; Zhang Gongliang ; Wang Jiaying ; Hou Hongman ; School of Food Science and Technology ; Dalian Polytechnic University
- 年：2016
- 作者机构：大连工业大学食品学院;
- 论文关键词：二氢香豆素 ; 群体感应 ; 蜂房哈夫尼菌 ; 生物膜
- 英文论文关键词：dihydrocoumarin ; quorum sensing ; hafnia alvei ; biofilm
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.3
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：98k
- 原文格式：O
- 会议级别：全国
摘要
群体感应是一种细菌细胞间信号传导和基因调控机制,能引起细菌致病性和食品腐败变质。因此,阻断细菌群体感应系统可以抑制群体感应控制的表型(例如:生物发光、生物膜形成、胞外酶合成)表达,控制食品的腐败。本研究中,主要选择分离自即食海参的特定腐败菌——蜂房哈夫尼菌,通过报告菌平板法和结晶紫染色法来探讨二氢香豆素抗生物膜及群体感应抑制活性的潜能。结果表明,二氢香豆素能够显著抑制由群体感应控制的CV026紫色杆菌素的产量而不抑制其生长,其抑制率达到70%。此外,二氢香豆素以浓度依赖的方式抑制蜂房哈夫尼菌游动能力(76%)和生物膜的形成(89%)。这些发现表明二氢香豆素可能作为一种新型群体感应抑制剂和/或抗生物膜制剂来调控食品的腐败过程,提高食品的安全性。
Quorum sensing(QS) is an intercellular signaling and gene regulatory mechanism,which is implicated in bacterial pathogenicity and food spoilage.Therefore,blocking bacterial QS system may prevent QS-controlled phenotypes(such as bioluminescence,biofilm formation,synthesis of extracellular enzyme) responsible for food spoilage.In the present study,we selected Hafnia alvei strain isolated from instant sea cucumber to be investigated and aimed to exhibit the anti-biofilm and quorum sensing inhibitory potentials of dihydrocoumarin by the methods of Standard disk-diffusion assay and crystal violet assay.The result showed that dihydrocoumarin inhibited QS-controlled violacein production in Chromobacterium violaceum 026 with 70%reduction without significantly affecting its growth.Moreover,dihydrocoumarin exhibited inhibition in swimming motility(76%) and biofilm formation(89%) of H.alvei in a concentration-dependent manner.These findings strongly suggest that dihydrocoumarin potentially could be developed as a new QS inhibitors and/or anti-biofilm agents to regulate the process of food corruption and increase food safety.
Quorum sensing(QS) is an intercellular signaling and gene regulatory mechanism,which is implicated in bacterial pathogenicity and food spoilage.Therefore,blocking bacterial QS system may prevent QS-controlled phenotypes(such as bioluminescence,biofilm formation,synthesis of extracellular enzyme) responsible for food spoilage.In the present study,we selected Hafnia alvei strain isolated from instant sea cucumber to be investigated and aimed to exhibit the anti-biofilm and quorum sensing inhibitory potentials of dihydrocoumarin by the methods of Standard disk-diffusion assay and crystal violet assay.The result showed that dihydrocoumarin inhibited QS-controlled violacein production in Chromobacterium violaceum 026 with 70%reduction without significantly affecting its growth.Moreover,dihydrocoumarin exhibited inhibition in swimming motility(76%) and biofilm formation(89%) of H.alvei in a concentration-dependent manner.These findings strongly suggest that dihydrocoumarin potentially could be developed as a new QS inhibitors and/or anti-biofilm agents to regulate the process of food corruption and increase food safety.
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