纳米氧化铁对作物的生物效应与分子机制探讨
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摘要
作物缺铁黄化时有发生,造成产量和品质明显下降,至今尚未发现一种真正既经济又有效的施铁方法。纳米氧化铁凭借其强吸附性、特殊的表面活性、穿透性等纳米效应,可望能更有效地被作物吸收,改善作物缺铁黄化症状。然而,纳米颗粒在水溶液中通常很难分散,呈团聚状态,也可能对植物产生毒性效应。本课题组研究了纳米氧化铁对缺铁逆境下玉米和柑橘的生理效应,发现高浓度的纳米氧化铁抑制了玉米根的伸长,并且在所有实验浓度下玉米叶绿素含量并未增加。同时,50 mg/L纳米氧化铁处理的柑橘的叶绿素含量也比对照组低,推断是纳米颗粒的团聚对植物产生了毒性效应。进而,研究柑橘根和叶与铁吸收转运有关基因的表达差异,发现不同种类纳米氧化铁的相关基因表达有差异。在根中,FRO2表达均增强,Nramp基因的表达均减少,这是可能纳米氧化铁可以补充部分铁,但仍不能满足植物需求并仍处于缺铁状态。在叶中,纳米氧化铁促进了FRO2的表达,但由于粒径太大造成转运困难,Nramp基因表达均增强,暗示纳米氧化铁团聚在根处,较少或几乎没有转运至叶部。
Iron oxide nanoparticles might have a great potential as a new iron fertilizer. However, the nanoparticles usually are difficult to disperse in aqueous solution, and easily aggregate. As a result, it is necessary to explore the biological effects and relevant molecular mechanisms of iron oxide namoparticles towrds plants for the agricultural application of nano-iron fertilizer. In our current research, it has been discovered that iron oxide nanoparticles did not show any positive effects on chlorophyll content of maize and citrus, and was difficult to translocate to leaves. More detailed researches is carrying out in order to conduct the agricultural application of this nano-fertilizer.
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