乌头碱分子印迹传感器的制备与应用
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- 英文论文题名：Preparation and Application of Aconitine Molecularly Imprinted Sensor
- 论文作者：熊彩云 ; 白慧萍 ; 曹秋娥
- 英文论文作者：Caiyun Xiong ; Huiping Bai ; Qiue Cao ; School of Chemical Science and Technology ; Yunnan University
- 年：2016
- 作者机构：云南大学化学科学与工程学院;
- 论文关键词：乌头碱 ; 分子印迹膜 ; 电化学传感器
- 英文论文关键词：aconitine ; molecularly imprinted membrane ; electrochemical sensor
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十三分会：复杂样品分离分析
- 语种：中文
- 分类号：O657.1
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十三分会 ; 复杂样品分离分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：331k
- 原文格式：D
- 会议级别：全国
摘要
乌头碱(ACO)是乌头、附子等植物中的主要活性成分,具有重要的药用价值,因此对乌头碱的分析研究引起了广泛关注~([1,2]),但未见用分子印迹传感器方法测定乌头碱的报道。本文结合电化学分析法与分子印迹技术的优点~([3]),以ACO为模板分子,甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,固定模板、单体以及交联剂的摩尔比为1:4:40,用原位聚合法,在裸玻碳电极上制得了一种乌头碱分子印迹电化学传感器。在优化的检测条件下,此传感器对乌头碱有灵敏的响应(见图1),其响应浓度范围为0.07~9.97μmol/L(见图2),检出限(以三倍信噪比表示)为0.03μmol/L。将该传感器用于加标血清以及尿液中乌头碱的测定,RSD在5.0%以内,加标回收率在90~110%以内。
A novel electrochemical sensor for the determination of aconitine(ACO) was prepared by modifying a ACO-imprinted membrane,which was synthesized by in situ polymerization with ACO as template,methacrylic acid(MAA) as functional monomer and ethylene glycol dimethacrylate(EGDMA) as cross linking agent,on glassy carbon electrode(GCE).Under the optimum conditions,the obtaining imprinted sensor possesses a sensitive response to aconitine in the concentration range of 0.35-49.85 μmol/L with the detection limit of 0.20 μmol/ L.
A novel electrochemical sensor for the determination of aconitine(ACO) was prepared by modifying a ACO-imprinted membrane,which was synthesized by in situ polymerization with ACO as template,methacrylic acid(MAA) as functional monomer and ethylene glycol dimethacrylate(EGDMA) as cross linking agent,on glassy carbon electrode(GCE).Under the optimum conditions,the obtaining imprinted sensor possesses a sensitive response to aconitine in the concentration range of 0.35-49.85 μmol/L with the detection limit of 0.20 μmol/ L.
引文
[1]Lara,F.J.;Airado-Rodríguez,D.;Moreno-González,D.;Huertas-Pérez,J.F.;García-Campa?a,A.M.Anal.Chim.Acta,2016,913:22
[2]El-Shazly,M;Tai,C.J.;Wu,T.Y.;Csupor,D.;et al.J.Food Drug Anal.2016,24(1):29
[3]Uzun,L.;Turner,A,P.F.Biosens.Bioelectronics,2016,76(1):131
[2]El-Shazly,M;Tai,C.J.;Wu,T.Y.;Csupor,D.;et al.J.Food Drug Anal.2016,24(1):29
[3]Uzun,L.;Turner,A,P.F.Biosens.Bioelectronics,2016,76(1):131