聚多巴胺-铅离子复合纳米材料构建超灵敏电化学免疫传感平台
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- 英文论文题名：An ultra-sensitive electrochemical immunoassay platform based on polydopamine-lead(Ⅱ) nanocomposites
- 论文作者：唐忠雪 ; 马占芳
- 英文论文作者：Zhongxue Tang ; Zhanfang Ma ; Department of Chemistry ; Capital Normal University
- 年：2016
- 作者机构：首都师范大学化学系;
- 论文关键词：聚多巴胺-铅(Ⅱ)纳米复合物 ; 壳聚糖-金纳米复合物 ; 癌胚抗原 ; 肿瘤标志物 ; 电化学
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十一分会：胶体与界面化学
- 语种：中文
- 分类号：O657.1
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十一分会 ; 胶体与界面化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：301k
- 原文格式：D
- 会议级别：全国
摘要
本文制备了新型的电化学信号物质(聚多巴胺-铅离子复合物,PDA-Pb~(2+))。首先通过碱性溶液中的O_2氧化多巴胺分子聚合(即氧化聚合)制得聚多巴胺,然后吸附铅离子,得到具有显著电化学信号的PDA-Pb~(2+)。通过滴涂成膜的方式在玻碳电极表面制备出PDA-Pb~(2+)膜,并吸附壳聚糖-金纳米复合物(Chit-AuNPs),改善电极的导电性能,同时固定抗体,构建出一种新颖的非标型电化学免疫传感平台,实现了肿瘤标志物癌胚抗原(CEA)的超灵敏检测。线性检测范围1 fg mL~(-1)-100ng mL~(-1),检出限0.26 fg mL~(-1)。该方法用于人血清检测的结果与ELISA的检测结果保持良好的一致性。
In this work,polydopamine-lead(Ⅱ) nanocomposites(PDA-Pb~(2+)) as a novel redox material was synthesized by using PDA,which was synthesized by the oxidative polymerization of dopamine hydrochloride in alkaline solution,to absorb Pb~(2+) ions and could produce an ultrahigh electrochemical signal.After PDA-Pb~(2+) form a stable film on glassy carbon electrode(GCE) by dipping,chitosan-gold nanocomposites(Chit-Au NPs) were immobilized for improving the conductivity of the electrode and the immobilization of antibodies,and then a new,label-free and electrochemical immunoassay platform was developed for the ultrasensitive detection of carcinoembryonic antigens(CEA).Under the optimized condition,the proposed immunosensor exhibited a linear detection range from 1 fg mL~(-1) to 100 ng mL~(-1) with a detection limit of 0.26 fg mL~(-1).For the human serum samples detection,this immunosensor showed satisfactory accuracy compared with ELISA.
In this work,polydopamine-lead(Ⅱ) nanocomposites(PDA-Pb~(2+)) as a novel redox material was synthesized by using PDA,which was synthesized by the oxidative polymerization of dopamine hydrochloride in alkaline solution,to absorb Pb~(2+) ions and could produce an ultrahigh electrochemical signal.After PDA-Pb~(2+) form a stable film on glassy carbon electrode(GCE) by dipping,chitosan-gold nanocomposites(Chit-Au NPs) were immobilized for improving the conductivity of the electrode and the immobilization of antibodies,and then a new,label-free and electrochemical immunoassay platform was developed for the ultrasensitive detection of carcinoembryonic antigens(CEA).Under the optimized condition,the proposed immunosensor exhibited a linear detection range from 1 fg mL~(-1) to 100 ng mL~(-1) with a detection limit of 0.26 fg mL~(-1).For the human serum samples detection,this immunosensor showed satisfactory accuracy compared with ELISA.
引文
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[2]D.Tang,R.Yuan,Y.Chai,Analyst 2008,133:933.
[3]T.Xu,N.Liu,J.Yuan,Z.Ma,Biosens.Bioelectron.2015,70:161.