新型金属-有机纳米复合物:聚亚甲基蓝-金并用于构建超灵敏电化学免疫平台
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- 英文论文题名：Novel metal-organic nanocomposites:poly(methylene blue)-Au used to fabricate an ultrasensitive electrochemical immunosensing platform
- 论文作者：单姣 ; 赵丽华 ; 马占芳
- 英文论文作者：Jiao Shan ; Lihua Zhao ; Zhanfang Ma ; Department of Chemistry ; Capital Normal University
- 年：2016
- 作者机构：首都师范大学化学系;
- 论文关键词：聚亚甲基蓝-金纳米复合物 ; 一锅法 ; 电化学免疫传感界面 ; 甲胎蛋白
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十一分会：胶体与界面化学
- 语种：中文
- 分类号：O657.1
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十一分会 ; 胶体与界面化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：381k
- 原文格式：D
- 会议级别：全国
摘要
超灵敏检测肿瘤标志物对于准确诊断癌症具有重要意义~([1])。本文通过一锅法利用氯金酸氧化聚合亚甲基蓝得到一种自身产生强电化学信号的探针材料,并利用石墨烯-金复合物做传感基底[2],构建了超灵敏电化学免疫传感界面,用于甲胎蛋白(AFP)的检测。AFP的线性检测范围1pg mL~(-1)-100ng mL~(-1),检出限19.6fg mL~(-1)。该免疫传感器的检测结果与ELISA检测结果有较好的一致性。
A kind of novel nanoprobe with innate strong electrochemical signal was synthesized using HAuCl_4 as oxidant and methylene blue as monomer.Graphene-Au composite was used as substrate in the immunosensor.α-fetoprotein was detected with a wide linear range from 1 pg mL~(-1) to 100 ng mL~(-1),and low detection limit of 19.6 fg mL~(-1) using the present platform.The assay results of clinical serum were well consistent with those obtained from ELISA method.
A kind of novel nanoprobe with innate strong electrochemical signal was synthesized using HAuCl_4 as oxidant and methylene blue as monomer.Graphene-Au composite was used as substrate in the immunosensor.α-fetoprotein was detected with a wide linear range from 1 pg mL~(-1) to 100 ng mL~(-1),and low detection limit of 19.6 fg mL~(-1) using the present platform.The assay results of clinical serum were well consistent with those obtained from ELISA method.
引文
[1]Li Y.;Zhong Z.;Chai Y.;Song Z.;Zhuo Y.;Su H.et al.Chem.Commun.2012,48:537.
[2]Song S.;Qin Y.;He Y.;Huang Q.;Fan C.;Chen H.Chem.Soc.Rev.2010,39:4234.
[2]Song S.;Qin Y.;He Y.;Huang Q.;Fan C.;Chen H.Chem.Soc.Rev.2010,39:4234.