CRISPR/Cas9系统介导的斑马鱼Hox基因突变体的建立及功能研究
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- 英文论文题名：The establish of Hox gene mutant using CRISPR/Cas9 and function research in Zebrafish
- 论文作者：孟祥丽 ; 张绪帅 ; 董雪红 ; 胡沛男 ; 白金尊 ; 祖尧
- 英文论文作者：MENGXiangli ; ZHANG Xushuai ; DONG Xuehong ; HU Peinan ; BAI Jinzun ; ZU Yao ; Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources ; Ministry of Education ; Shanghai Ocean University
- 年：2016
- 作者机构：水产种质资源发掘与利用教育部重点实验室;上海海洋大学水产与生命学院;
- 论文关键词：CRISPR/Cas9 ; 基因敲除 ; 大片段删除 ; Hox基因(簇) ; 斑马鱼
- 英文论文关键词：CRISPR/Cas9 ; knock out ; fragment deletion ; Hox gene(cluster) ; zebrafish
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：537k
- 原文格式：D
- 会议级别：全国
摘要
Hox基因是一类在早期胚胎发育过程中调控身体形态构建的一大类转录因子家族。当Hox基因产生突变时,往往会导致早期胚胎发育的畸形。无脊椎动物的基因组中只有一个基因簇,而脊椎动物在进化过程中染色体发生加倍过程,最终在四足动物中含有4个Hox基因簇,在硬骨鱼类的斑马鱼中由于基因缺失等原因最终形成了7个Hox基因簇。本人利用CRISPR/Cas9技术对斑马鱼Hox基因(簇)进行敲除或片段删除。其中针对单位点所进行的敲除,敲除的靶位点有Hox B7a、Hox C1a、Hox C3a等多个位点,同时针对片段进行的敲除有Hox Ba、Hox Bb2个基因簇。这些纯合突变体的获得可以帮助我们解答由于硬骨鱼类染色体加倍而产生的Hox基因簇加倍对硬骨鱼类早期胚胎发育的作用以及硬骨鱼类所特有的Hox基因的具体功能。
Hox gene is a group of transcript factor family, which control the body construction in embryonic development. The mutations of Hox gene often lead to the malformation of early embryos. The genome of invertebrate often has only one Hox gene cluster, while the vertebrate has at least 4 Hox gene clusters in the genome as the duplication of Chromosome. The zebrafish, a kind of teleosts, which went through another chromosome duplication, finally produced 7 gene clusters as for the deletion or mutation of genome during the evolution..I successfully edited the zebrafish Hox gene(cluster) with CRISPR/Cas9 system. On one hand, I knock out the target site of Hox B7 a 、 Hox C1 aand Hox C3 a. On the other hand, I deleted the fragment of Hox Ba cluster,Hox Bb cluster I think we could have a better understanding of the functional differentiation of Hox gene clusters duplication and the specific Hox genes function in teleosts by further study for these Hox gene(cluster) homozygous mutation.
Hox gene is a group of transcript factor family, which control the body construction in embryonic development. The mutations of Hox gene often lead to the malformation of early embryos. The genome of invertebrate often has only one Hox gene cluster, while the vertebrate has at least 4 Hox gene clusters in the genome as the duplication of Chromosome. The zebrafish, a kind of teleosts, which went through another chromosome duplication, finally produced 7 gene clusters as for the deletion or mutation of genome during the evolution..I successfully edited the zebrafish Hox gene(cluster) with CRISPR/Cas9 system. On one hand, I knock out the target site of Hox B7 a 、 Hox C1 aand Hox C3 a. On the other hand, I deleted the fragment of Hox Ba cluster,Hox Bb cluster I think we could have a better understanding of the functional differentiation of Hox gene clusters duplication and the specific Hox genes function in teleosts by further study for these Hox gene(cluster) homozygous mutation.
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