野茉莉(Styrax japonicus)组培快繁技术研究
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- 英文论文题名：Study on Embroy Culture of Styrax japonicus
- 论文作者：王文君 ; 刘庆华 ; 王奎玲 ; 张翠萍
- 英文论文作者：WANG Wen-jun ; LIU Qing-hua ; WANG Kui-ling ; ZHANG Cui-ping ; College of Landscape Architecture and Forestry ; Qingdao Agricultural University
- 年：2016
- 作者机构：青岛农业大学园林与林学院;
- 论文关键词：野茉莉 ; 种胚苗 ; 组培快繁
- 英文论文关键词：Styrax japonicus ; Tissue culture ; Rapid propagation system
- 会议召开时间：2016-07-19
- 会议录名称：中国观赏园艺研究进展2016
- 语种：中文
- 分类号：S567.19
- 学会代码：EGYP
- 会议名称：2016年中国观赏园艺学术研讨会
- 会议地点：中国湖南长沙
- 主办单位：中国园艺学会观赏园艺专业委员会、国家花卉工程技术研究中心
- 学会名称：中国园艺学会
- 页数：4
- 文件大小：512k
- 原文格式：O
- 会议级别：全国
摘要
本试验以野茉莉种胚苗为材料,对其进行离体快速繁殖研究。结果表明:用2g·L~(-1)NaClO处理低温贮藏的野茉莉种子15min即可达到较好的杀菌效果。增殖效果良好的培养基是MS+BA 0.1mg·L~(-1)+IBA0.3mg·L~(-1),增殖系数达到5.55,芽多且粗壮;采用两阶段诱导野茉莉生根,100mg·L~(-1)IBA处理7d,转入1/2MS培养,生根数最多,生根率为77.8%。泥炭土和珍珠岩比例为1:1作为基质,以穴盘作为容器,组培苗移栽成活率达到80%,组培苗叶色鲜艳,长势良好。该研究为品种的大规模繁殖和大面积推广提供了参考。
Experiments on tissue culture of Styrax japonicus was conducted by taking the seeds as explants.The results show that the 2g · L~(-1) NaClO handing of seeds that being cryogenic storage was better,the best soak time was 5 min.MS medium with0.1 mg · L~(-1) BA and 0.3mg · L~(-1) IBA was the most suitable for the propagation of Styrax japonicas,and the propagation rate reached 5.55 times,bud is abundant and healthy.Treatments with 100 mg/L IBA for 3 days had the best root induction effect,topped to 77.8%of rooting rate.Taking nutrition pot as container,peat soil and perlite as transplanting medium(1:1),the transplant survival rate was over 80%.There were many lateral roots and the plantlets which grew rapidly and vigorously.This method was suitable for tissue culture of Styrax japonicus and laid foundations for its large-scaled propagation and large area promotion.
Experiments on tissue culture of Styrax japonicus was conducted by taking the seeds as explants.The results show that the 2g · L~(-1) NaClO handing of seeds that being cryogenic storage was better,the best soak time was 5 min.MS medium with0.1 mg · L~(-1) BA and 0.3mg · L~(-1) IBA was the most suitable for the propagation of Styrax japonicas,and the propagation rate reached 5.55 times,bud is abundant and healthy.Treatments with 100 mg/L IBA for 3 days had the best root induction effect,topped to 77.8%of rooting rate.Taking nutrition pot as container,peat soil and perlite as transplanting medium(1:1),the transplant survival rate was over 80%.There were many lateral roots and the plantlets which grew rapidly and vigorously.This method was suitable for tissue culture of Styrax japonicus and laid foundations for its large-scaled propagation and large area promotion.
引文
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13.Vihera-Aarnio A,Veiling P.2001.Micropropagated silver birches(Betula pendula)in the field performance and clonal differences[J].Silva Fennica,35(4):385-401.
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16.郑玉梅,刘青林.2001.木本观赏植物离体快速繁殖技术的进展[J].北京林业大学学报,76-79.
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2.崔纪如,赵世伟.野茉莉的引种栽培.北京市植物园.
3.高效民.1984.植物离体培养中染色体的变异[J].细胞生物学杂志,6(1):5-12.
4.刘英,曾炳山,裘珍飞,等.2003.西南桦以芽组培快繁研究[J].林业科学研究.16(6):715-719.
5.芦建国,梁同江,李舒仪.2009.福建武夷山国家级自然保护区野茉莉科植物资源利用前景[J].福建林业科技,36(3):182-185.
6.马燕,韩瑞超,臧德奎,等.2012.木本观赏植物组织培养研究进展[J].安徽农业科学,40(4):1956-1958.
7.马祎,刘青林.2001,本观赏植物离体快速繁殖的关键技术研究[J].北京林业大学学报,23:21-23.
8.王桂萍,胡光平,韩堂松,等.2013.赤霉素处理对野茉莉种子萌发及幼苗生长的影响[J].林业科技,38(5):11-13.
9.王奎玲,刘庆超,李俊卿,等.2010.玉玲花的组织培养与快速繁殖[J].植物生理学通讯,46(9):959-960.
10.王清民,彭伟秀,张俊佩,等.2006.核桃试管嫩茎生根的形态结构及激素调控研究[J].园艺学报,33(2):255-259.
11.SNIR I.1983.A micropropagat ion syst em f or sour cherry[J].S cientia Hort ic,22:311-386.
12.孙宁,许雪峰,韩振海,等.2008.酸樱桃组培快繁技术体系的研究[J].西北植物学报,28(1):47-51.
13.Vihera-Aarnio A,Veiling P.2001.Micropropagated silver birches(Betula pendula)in the field performance and clonal differences[J].Silva Fennica,35(4):385-401.
14.王战,方振福.1984.中国植物志[M].北京科学出版社,20(2):357.
15.张亮亮,柳新红,林新春,等.2013.白花树组织培养技术研究[J].浙江林业科技,33(3):17-21.
16.郑玉梅,刘青林.2001.木本观赏植物离体快速繁殖技术的进展[J].北京林业大学学报,76-79.
17.朱靖杰.1995.香蕉组织培养中变异的发生与控制途径[J].果树科学,12(2):120-122.
18.张素勤,邹志荣,耿广东,等.2007.几种因素对非洲菊试管苗生根的影响[J].西南大学学报,29(8):77-81.