一种基于丹磺酰氨的硫化氢荧光探针及细胞成像应用
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- 英文论文题名：A Colorimetric and Ratiometric Fluorescent Probe for Mercury(Ⅱ) in Lysosome
- 论文作者：韩勰 ; 盛晓乐 ; 刘盛华 ; 尹军
- 英文论文作者：Yufeng Zhang ; ShengHua Liu ; Jun Yin ; Key Laboratory of Pesticide and Chemical Biology of the Ministry of Education ; College of Chemistry ; Central China Normal University
- 年：2016
- 作者机构：华中师范大学化学学院农药与化学生物学教育部重点实验室,华中师范大学;
- 论文关键词：硫化氢 ; 荧光探针 ; 生物成像
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十八分会：化学生物学
- 语种：中文
- 分类号：O657.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十八分会 ; 化学生物学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：217k
- 原文格式：D
- 会议级别：全国
摘要
硫化氢被认为是体内除了NO和CO之外的第三种信号分子~1。硫化氢在体内各种生理过程和病理过程中起着重要的作用,如血管舒张、细胞凋亡、神经传递,胰岛素分泌和炎症等~2。另一方面,作为一种具有臭鸡蛋味的有毒气体,高浓度的硫化氢也会对人体产生伤害,会导致中枢神经系统损伤和窒息,甚至导致死亡。因此,开发一种可以高选择性和灵敏性的检测硫化氢并可以应用于生物系统的检测方法是非常重要的。在本文中,我们设计合成了一种基于PET的硫化氢荧光探针。该探针能够高选择性识别硫化氢并能够对细胞内的硫化氢进行生物成像。结果表明该硫化氢探针可以作为一种有效的检测细胞内的硫化氢的工具。
Hydrogen sulfide is considered as the third signaling molecule in vivo~1 and it plays an important role in various physiological processes and pathological processes in vivo, such as vasodilation, apoptosis, neurotransmission, ischemia/reperfusion-induced injury, insulin secretion and inflammation.~2 Developing a highly selective and sensitive method that can detect H_2S in the biological system is very important. In this work, a colorimetric and "turn-on" fluorescent probe is developed. Furthermore, this probe displays a highly selective response to H_2S and possesses good capability for bioimaging H_2S without interference in living cells.
Hydrogen sulfide is considered as the third signaling molecule in vivo~1 and it plays an important role in various physiological processes and pathological processes in vivo, such as vasodilation, apoptosis, neurotransmission, ischemia/reperfusion-induced injury, insulin secretion and inflammation.~2 Developing a highly selective and sensitive method that can detect H_2S in the biological system is very important. In this work, a colorimetric and "turn-on" fluorescent probe is developed. Furthermore, this probe displays a highly selective response to H_2S and possesses good capability for bioimaging H_2S without interference in living cells.
引文
[1]Kimura,H.;Nagai,Y.;Umemura K.;Kimura,Y.Antioxid.Redox Signaling,2005,7:795.
[2]Shibuya,N.;Tanaka,M.;Yoshida,M.;Ishii,K.;Kimura,H.Antioxid.Redox Signaling.2009,11:703.
[2]Shibuya,N.;Tanaka,M.;Yoshida,M.;Ishii,K.;Kimura,H.Antioxid.Redox Signaling.2009,11:703.