响应面法优化甜玉米芯多糖的硫酸酯化工艺研究
详细信息 查看官网全文
- 英文论文题名：Optimization on sulfated technology of polysaccharides from sweet corncobs by response surface method
- 论文作者：高爽 ; 马永强 ; 王鑫
- 英文论文作者：Gao Shuang ; Ma Yongqiang ; Wang Xin ; College of Food Engineering ; Harbin University of Commerce
- 年：2016
- 作者机构：哈尔滨商业大学食品工程学院;
- 论文关键词：甜玉米芯 ; 多糖 ; 硫酸酯化 ; 响应面法
- 英文论文关键词：sweet corncobs ; polysaccharides ; sulfation ; response surface methodology
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.2
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：106k
- 原文格式：O
- 会议级别：全国
摘要
为了进一步提高甜玉米芯多糖的生物活性,采用浓硫酸法对甜玉米芯多糖进行硫酸酯化改性。通过单因素实验,以取代度为指标,筛选出影响甜玉米芯多糖硫酸酯化的三个重要因素,即浓硫酸体积、反应时间和硫酸铵用量,根据响应面Design-Expert软件中的Box-Benhnken中心组合实验设计原理,设计三因素三水平的响应面优化实验,确定了最佳硫酸酯化工艺条件。结果表明,最佳硫酸酯化工艺条件为:浓硫酸体积6mL,反应时间25min,硫酸铵用量0.06g,此条件下取代度达到1.158。红外光谱分析得到硫酸酯化甜玉米芯多糖中引入了硫酸基团。最佳条件下获得的硫酸酯化甜玉米芯多糖溶解性较好,这为其更广泛的应用提供了可能。
Polysaccharides from sweet corncobs were sulfated modification with the method of sulfuric acid to improve its biological activity.Based on the degree of substitution,three important factors were selected which affecting sulfated modification of polysaccharides from sweet corncobs through single factor experiment that contained the volume of sulfuric acid,reaction time and the dosage of ammonium sulfate.Through the response surface software Design-Expert for a further optimization,a series of experimental programs were designed according to the principle of Box-Benhnken central combination design to study the optimum conditions.Results showed that the optimum conditions were as follows:the volume of sulfuric acid,the reaction time and the dosage of ammonium sulfate,were 6mL,25 min and 0.06 g,respectively.Under this conditions,the degree of substitution was up to 1.158.Sulfate groups were bringed into polysaccharides from sweet corncobs by infrared spectrum analysis.The solubility of sulfated modification of polysaccharides had good solubility with optimum conditions for more extensive applications.
Polysaccharides from sweet corncobs were sulfated modification with the method of sulfuric acid to improve its biological activity.Based on the degree of substitution,three important factors were selected which affecting sulfated modification of polysaccharides from sweet corncobs through single factor experiment that contained the volume of sulfuric acid,reaction time and the dosage of ammonium sulfate.Through the response surface software Design-Expert for a further optimization,a series of experimental programs were designed according to the principle of Box-Benhnken central combination design to study the optimum conditions.Results showed that the optimum conditions were as follows:the volume of sulfuric acid,the reaction time and the dosage of ammonium sulfate,were 6mL,25 min and 0.06 g,respectively.Under this conditions,the degree of substitution was up to 1.158.Sulfate groups were bringed into polysaccharides from sweet corncobs by infrared spectrum analysis.The solubility of sulfated modification of polysaccharides had good solubility with optimum conditions for more extensive applications.
引文