高效液相色谱法测定添加剂预混合饲料中左旋咪唑
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- 英文论文题名：Determination of Levamisole in Additive Premix Feeds by HPLC
- 论文作者：吴荔琴 ; 林海丹 ; 方秋华 ; 伍宏凯 ; 刘佩怡 ; 梁祺琦 ; 何滢
- 英文论文作者：WU Li-qin ; LIN Hai-dan ; FANG Qiu-hua ; WU Hong-kai ; LIU Pei-yi ; LIANG Qi-qi ; Guangdong Institute for Veterinary Drug and Feedstuffs Control
- 年：2016
- 作者机构：广东省兽药饲料质量检验所;
- 论文关键词：左旋咪唑 ; 检测 ; 添加剂预混合饲料 ; 高效液相色谱法
- 英文论文关键词：levamisole ; determination ; additive Premix feeds ; HPLC
- 会议召开时间：2016-09-18
- 会议录名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会论文集
- 语种：中文
- 分类号：S816.17
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物药品学分会第五届全国会员代表大会暨2016年学术年会
- 会议地点：中国四川成都
- 主办单位：中国畜牧兽医学会动物药品学分会
- 学会名称：中国畜牧兽医学会
- 页数：7
- 文件大小：663k
- 原文格式：O
- 会议级别：全国
摘要
建立了添加剂预混合饲料中左旋咪唑检测的高效液相色谱法。样品中左旋咪唑分散于氢氧化钠水溶液,用乙酸乙酯反萃取,萃取液氮气吹干后,流动相复溶,强离子交换色谱柱分离,紫外检测。结果显示,左旋咪唑在0.05μg/mL~10μg/mL的浓度范围内线性关系良好(r>0.999 9),添加回收率在76.6%~103%之间,RSD<6%,检测限为0.4 mg/kg,定量限为1 mg/kg。
A high-performance liquid chromatography method for determination of levamisole in additive Premix feeds was established.Levamisole was extracted by ethyl acetate from alkaline water solution,dissolved by mobile phase after concentration,separated by the SCX column,and detected by UV.The results showed that in a concentration rang of Linearity of 0.05μg/mL~10μg/mL,the linear regression was good(r>0.999 9).Recoveries of levamisole from spiked additive Premix feeds at different concentration were between 76.6%~103%,RSD<10%.The LOD was 0.4 mg/kg and the LOQ was 1 mg/kg.
A high-performance liquid chromatography method for determination of levamisole in additive Premix feeds was established.Levamisole was extracted by ethyl acetate from alkaline water solution,dissolved by mobile phase after concentration,separated by the SCX column,and detected by UV.The results showed that in a concentration rang of Linearity of 0.05μg/mL~10μg/mL,the linear regression was good(r>0.999 9).Recoveries of levamisole from spiked additive Premix feeds at different concentration were between 76.6%~103%,RSD<10%.The LOD was 0.4 mg/kg and the LOQ was 1 mg/kg.
引文
[1]中国兽药典委员会.中华人民共和国兽药典兽药使用指南[M].北京:中国农业出版社出版,2010.1:142~143
[2]中华人民共和国农业部公告第235号.动物性食品中兽药最高残留限量
[3]林维宣,田苗,隋凯等.高效液相色谱法测定牛乳中左旋咪唑[J].中国乳品工业,2011,29(1):24~26
[4]曾建亭,钟小燕.HPLC法测定盐酸左旋咪唑片的含量及溶出度.中国热带医学,2010,10(5):628~629
[5]曾勇,金秀娥,卢芳等.猪鸡组织中左旋咪唑残留的HPLC检测方法研究[J].中国兽药杂志,2010,44(8):17~21
[6]B.Wyhowski de Bukanski,J.M.Degroodt,and H.Beernaert.Determination of lecamisole and thiabendazole in meat by HPLC and photodiode array detection[J].Z Lebensm Unters Forsch,1991,193:545-547
[7]徐静,肖珊珊,董伟峰等.两次液液萃取-气相色谱-质谱联用法测定动物肝脏中左旋咪唑残留[J].色谱,2012,30(9):922-925
[8]Brigitte Wyhowski de Bukanski,Jean-Marie Degroodt,and Hedwig Beemaert.Determination of lecamisole and thiabendazole in meat by HPLC and photodiode array detection[J].Z Lebensm Unters Forsch,1991,193:545-547
[9]A.E.Tyrpenou,E.M.Xylouri-Frangiadaki.Determination of Levamisole in Sheep Muscle Tissue by High-Performance Liquid Chromatography and Photo Diode Array Detection[J].Chromatographia,2006,63:321-326
[10]薄海波,庞国芳,雒丽丽等.超高效液相色谱-串联质谱法测定牛奶和奶粉中残留的左旋咪唑[J].色谱,2009,27(2):149-152
[11]全国饲料工业标准化技术委员会.GB/T 23182-2008[S].饲料中兽药及其他化学物监测试验规程.北京:中国标准出版社,2008-12-31.
[2]中华人民共和国农业部公告第235号.动物性食品中兽药最高残留限量
[3]林维宣,田苗,隋凯等.高效液相色谱法测定牛乳中左旋咪唑[J].中国乳品工业,2011,29(1):24~26
[4]曾建亭,钟小燕.HPLC法测定盐酸左旋咪唑片的含量及溶出度.中国热带医学,2010,10(5):628~629
[5]曾勇,金秀娥,卢芳等.猪鸡组织中左旋咪唑残留的HPLC检测方法研究[J].中国兽药杂志,2010,44(8):17~21
[6]B.Wyhowski de Bukanski,J.M.Degroodt,and H.Beernaert.Determination of lecamisole and thiabendazole in meat by HPLC and photodiode array detection[J].Z Lebensm Unters Forsch,1991,193:545-547
[7]徐静,肖珊珊,董伟峰等.两次液液萃取-气相色谱-质谱联用法测定动物肝脏中左旋咪唑残留[J].色谱,2012,30(9):922-925
[8]Brigitte Wyhowski de Bukanski,Jean-Marie Degroodt,and Hedwig Beemaert.Determination of lecamisole and thiabendazole in meat by HPLC and photodiode array detection[J].Z Lebensm Unters Forsch,1991,193:545-547
[9]A.E.Tyrpenou,E.M.Xylouri-Frangiadaki.Determination of Levamisole in Sheep Muscle Tissue by High-Performance Liquid Chromatography and Photo Diode Array Detection[J].Chromatographia,2006,63:321-326
[10]薄海波,庞国芳,雒丽丽等.超高效液相色谱-串联质谱法测定牛奶和奶粉中残留的左旋咪唑[J].色谱,2009,27(2):149-152
[11]全国饲料工业标准化技术委员会.GB/T 23182-2008[S].饲料中兽药及其他化学物监测试验规程.北京:中国标准出版社,2008-12-31.