液体辅助离子化系统对酶催化的机理研究
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摘要
<正>酶催化反应在动植物的生命过程中具有重要作用,但其机理复杂种类繁多,给研究及评价工作带来了压力。常规的研究方法是色谱分离,耗时费力,需要人们建立能够直接给出分子结构信息的快速检测手段,进行反应评价及催化剂快速筛选~(1,2),还可以获得可靠的中间体信息,对反应动力学及机理研究提供数据。近年敞开式常压质谱技术发展迅速,为样品的快速离子化检测提供了新途径,也为反应评价提供了新手段~(3,4)。电喷雾离子源是较为成熟的用于生物分子
引文
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