一种含吡啶环的pH荧光探针的设计与应用
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- 英文论文题名：An oxazole ring containing pH fluorescent probe derived from pyridine as the protonation site
- 论文作者：付鲸铭 ; 金明 ; 万德成
- 英文论文作者：Jingming Fu ; Ming Jin ; Decheng Wan ; Department of Polymer Science ; School of Materials Science and Engineering ; Tongji University
- 年：2016
- 作者机构：同济大学材料科学与工程学院高分子系;
- 论文关键词：荧光探针 ; 吡啶 ; pH
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十七分会：光化学
- 语种：中文
- 分类号：O657.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十七分会 ; 光化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：227k
- 原文格式：D
- 会议级别：全国
摘要
本文设计并合成了一种以吡啶基团作为离子受体[1]的pH荧光探针TP,分子结构如Fig.1所示,通过引入一个三乙二醇单甲醚基团增加分子的亲水性;同时引入苯乙烯基团增加分子的共轭体系的尺寸,使分子的双光子吸收截面增大。这样,分子结构中含有H+离子受体,可以保证分子的离子选择性;带有较好亲水性的三乙二醇单甲醚能保证探针分子较好的亲水性[2]。分子在不同pH缓冲液中的紫外吸收谱如Fig.2所示,随着pH值的下降,TP的紫外吸收在361nm处变弱的同时在305nm和425nm处变强。TP的荧光光谱如Fig.3所示,在pH值下降的同时,分子在500nm处的荧光强度明显下降。这些结果表明TP在微酸性环境中对pH值的变化有较为明显的响应性。由于该离子探针在生物体内弱酸性环境有高度敏感性和高的水溶性,对于化学生物学的研究有很好的应用价值。
This contribution reports on the synthesis of a pH fluorescent probe(TP) derived from pyridine as the protonation site. The water solubility of the probe is excellent especially in weak acid environment which is achieved by introducing a hydrophilic group(triethylene glycol monomethyl ether) to the structure. With a decrease in the pH value, the UV absorption of TP gradually decreased nearby 361 nm with a slightly red shift of λmax and concomitantly increased at 305 nm and 425 nm. The fluorescence intensity of the emission spectra at 500 nm decreased with decreased pH value. With good water solubility and highly sensitivity to pH in weak acid environment, the probe is supposed to be attractive in chemicobiology research.
This contribution reports on the synthesis of a pH fluorescent probe(TP) derived from pyridine as the protonation site. The water solubility of the probe is excellent especially in weak acid environment which is achieved by introducing a hydrophilic group(triethylene glycol monomethyl ether) to the structure. With a decrease in the pH value, the UV absorption of TP gradually decreased nearby 361 nm with a slightly red shift of λmax and concomitantly increased at 305 nm and 425 nm. The fluorescence intensity of the emission spectra at 500 nm decreased with decreased pH value. With good water solubility and highly sensitivity to pH in weak acid environment, the probe is supposed to be attractive in chemicobiology research.
引文
[1]Meng,X.;Wang,S.;Li,Y.Chem.Commun.,2012,48(35):4196-4198.
[2]Daniel A.Sheik,Lauren Brooks,Kristen Frantzen.ACS Nano,2015,9(2):1829-1836.
[2]Daniel A.Sheik,Lauren Brooks,Kristen Frantzen.ACS Nano,2015,9(2):1829-1836.