产纤维素酶酵母菌的筛选及鉴定
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- 英文论文题名：Screening of Cellulase Producing Strains and Identification
- 论文作者：张璐璐 ; 卫旭彪 ; 马广 ; 张日俊
- 英文论文作者：Zhang Lu-lu ; WEI Xu-biao ; Ma Guang ; ZHANG Ri-jun ; Laboratory of Feed Biotechnology ; State Key Laboratory of Animal Nutrition ; China Agricultural University
- 年：2016
- 作者机构：中国农业大学饲料生物技术实验室动物营养学国家重点实验室;
- 论文关键词：纤维素酶 ; 酵母菌 ; 筛选 ; 生物学特性
- 英文论文关键词：cellulase ; yeast ; screening biological ; characteristics
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：Q93
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：71k
- 原文格式：O
- 会议级别：全国
摘要
本研究旨在筛选出产纤维素酶较高的酵母菌,并对其生物学特性探讨。试验从生境中筛选到产纤维素酶较高一株酵母菌,通过菌株形态学和分子生物学对该菌株进行了鉴定,并对这株菌的耐酸性、耐模拟肠液性、产酶特性和抑菌能力进行评定。结果表明,所筛酵母菌Y3为酿酒酵母菌,产纤维素内切葡聚糖酶酶活为3.27 U/mL,纤维素外切葡聚糖酶酶活为2.22 U/mL,木聚糖酶酶活为4.21 U/mL,耐酸性,耐模拟胃液较强,而耐模拟肠液能力较弱,不能产生抗金黄色葡萄球菌的物质。结果提示,本试验筛选到酿酒酵母菌Y3是一株较高产纤维素酶菌株并具有良好的生物学特性,对于提高粮食加工副产物的利用率具有非常重要的现实意义。
This experiment was conducted to screen a high cellulose producing yeast strain and study its biological characteristics.In this research,a yeast strain that produce more cellulose were isolated from habitats.We identified this strain base on morphological features and molecular biology features.Then acid resistance,simulated small intestine fluid resistance,cellulose activity and antimicrobial activity were determined.The results showed that:strain Y3 was a saccharomyces cerevisiae with strong acid resistance and artificial gastric acid resistance but poor small intestine fluid resistance.The endoglucanase activity,exoglucanase activity and xylanase activity of the fermented corn fiber and soybean dregs were 3.27 U/mL,2.22 U/mL and 4.21 U/mL,respectively.Moreover,this strain showed no inhibitory activity against staphylococcus aureus.These results indicated that acchawmyces cerevisiae Y3 can produce high yield of cellulose and has good biological characteristics.This research shows great value in improving the utilization rate of food processing by-products.
This experiment was conducted to screen a high cellulose producing yeast strain and study its biological characteristics.In this research,a yeast strain that produce more cellulose were isolated from habitats.We identified this strain base on morphological features and molecular biology features.Then acid resistance,simulated small intestine fluid resistance,cellulose activity and antimicrobial activity were determined.The results showed that:strain Y3 was a saccharomyces cerevisiae with strong acid resistance and artificial gastric acid resistance but poor small intestine fluid resistance.The endoglucanase activity,exoglucanase activity and xylanase activity of the fermented corn fiber and soybean dregs were 3.27 U/mL,2.22 U/mL and 4.21 U/mL,respectively.Moreover,this strain showed no inhibitory activity against staphylococcus aureus.These results indicated that acchawmyces cerevisiae Y3 can produce high yield of cellulose and has good biological characteristics.This research shows great value in improving the utilization rate of food processing by-products.
引文
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[2]张亮,池振明.1株产纤维素酶海洋酵母菌的筛选、鉴定及发酵条件优化[J].中国海洋大学学报(自然科学版),2007(S2):101-108.
[3]SARAWAN S,MAHAKHAN P,JINDAMORAKOT S,et al.Candida konsanensis sp.nov.,a new yeast species isolated from Jasminum adenophyllum in Thailand with potentially carboxymethyl cellulase-producing capability[J],World Journal of Microbiology and Biotechnology,2013,29(8):1481-1486.
[4]李坤平,潘天玲,黄克瀛.半纤维素酶水解产物木糖醇发酵酵母菌的筛选[J].广东药学院学报,2004,20(1):4-6.
[2]张亮,池振明.1株产纤维素酶海洋酵母菌的筛选、鉴定及发酵条件优化[J].中国海洋大学学报(自然科学版),2007(S2):101-108.
[3]SARAWAN S,MAHAKHAN P,JINDAMORAKOT S,et al.Candida konsanensis sp.nov.,a new yeast species isolated from Jasminum adenophyllum in Thailand with potentially carboxymethyl cellulase-producing capability[J],World Journal of Microbiology and Biotechnology,2013,29(8):1481-1486.
[4]李坤平,潘天玲,黄克瀛.半纤维素酶水解产物木糖醇发酵酵母菌的筛选[J].广东药学院学报,2004,20(1):4-6.